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The map label for this gene is thiDE [H]

Identifier: 85374878

GI number: 85374878

Start: 2081893

End: 2082702

Strand: Direct

Name: thiDE [H]

Synonym: ELI_10255

Alternate gene names: 85374878

Gene position: 2081893-2082702 (Clockwise)

Preceding gene: 85374877

Following gene: 85374882

Centisome position: 68.21

GC content: 65.43

Gene sequence:

>810_bases
ATGAGCCGCCAGCCACCGCGTATTCTCGCGATTGCCGGCTCCGACAGTTCGGGCGGCGCAGGGATCCAGGCCGATATCAA
GACGATTACCATGCTGGGCGGTTATGCGATGACCGCCATCACTGCGGTCACGGCCCAGAACTCGGTCGGCGTACAGGGCA
TCACGCCGCTCGATGGCGCATTCGTCGGCCAGCAGATCGCATCGGCAATCGATGATATCGGCGTCGACGCGATCAAGATC
GGCATGCTTCAGAACGAGGACATTATCCTCGCCGTGGCCAACGCTTTGGAAGAGATCGATCCGCAAGTTCCCATCGTGCT
CGACCCGGTCATGGTGGCGACATCGGGTGCGCCGCTGGTGGTGCAGGATGCCGTCGCTACAATGCGCGAGCGGCTGTTCA
CCAAGGCACGGCTGCTGACTCCCAACCTGCCCGAACTCGAATTGCTGGCTGGCAAGCAATTGCGCACGACCGAGCTCGTC
CGCGAGGCAGCCGAAGATCTTGCGCGCCATTACGGCTGCGATGTGCTGGCCAAGGGCGGGCACACCGCTGACGAGCGGAT
CATCGATGTGCTGGTGCCGGTCGGCGGGCGCATGGCGCAATTCGACGATGCGCGGATCGACACGCGCCACACCCATGGCA
CCGGCTGCACGCTGTCATCCGCCATCGCGACCCTGCTCGGCCACGGCCAGCCGATGGAGCACGCCGTGCGGCTGGCGCGG
CGATTCGTCCGTGAGGCGATCGAGAACGCGCCCGGTTTCGGCGACGGGGCCGGGCCCCTCGGGCACCAGGCGGTACGGTT
GAAGAGCTAG

Upstream 100 bases:

>100_bases
ATCTCTGCCCCAATTGCGGCGGCGAACTGATGGATCGCCCGACCCGCGCGGCCAAACTGCTGGAGAAGTTTCCCGCGAGT
ACCGAACGGAAGCTCAGGAA

Downstream 100 bases:

>100_bases
CGGTCGATGTCGTAGAAGTCGGCGATGTGGCCCCACGCCTCATCCGCCGTCTCGCACCACGTAATCAGGTCGAGGTCCTG
TTTCGAGATCGTGCCTTCCT

Product: hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSRQPPRILAIAGSDSSGGAGIQADIKTITMLGGYAMTAITAVTAQNSVGVQGITPLDGAFVGQQIASAIDDIGVDAIKI
GMLQNEDIILAVANALEEIDPQVPIVLDPVMVATSGAPLVVQDAVATMRERLFTKARLLTPNLPELELLAGKQLRTTELV
REAAEDLARHYGCDVLAKGGHTADERIIDVLVPVGGRMAQFDDARIDTRHTHGTGCTLSSAIATLLGHGQPMEHAVRLAR
RFVREAIENAPGFGDGAGPLGHQAVRLKS

Sequences:

>Translated_269_residues
MSRQPPRILAIAGSDSSGGAGIQADIKTITMLGGYAMTAITAVTAQNSVGVQGITPLDGAFVGQQIASAIDDIGVDAIKI
GMLQNEDIILAVANALEEIDPQVPIVLDPVMVATSGAPLVVQDAVATMRERLFTKARLLTPNLPELELLAGKQLRTTELV
REAAEDLARHYGCDVLAKGGHTADERIIDVLVPVGGRMAQFDDARIDTRHTHGTGCTLSSAIATLLGHGQPMEHAVRLAR
RFVREAIENAPGFGDGAGPLGHQAVRLKS
>Mature_268_residues
SRQPPRILAIAGSDSSGGAGIQADIKTITMLGGYAMTAITAVTAQNSVGVQGITPLDGAFVGQQIASAIDDIGVDAIKIG
MLQNEDIILAVANALEEIDPQVPIVLDPVMVATSGAPLVVQDAVATMRERLFTKARLLTPNLPELELLAGKQLRTTELVR
EAAEDLARHYGCDVLAKGGHTADERIIDVLVPVGGRMAQFDDARIDTRHTHGTGCTLSSAIATLLGHGQPMEHAVRLARR
FVREAIENAPGFGDGAGPLGHQAVRLKS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=44.1860465116279, Blast_Score=178, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=35.9259259259259, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324997, Length=252, Percent_Identity=40.0793650793651, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324517, Length=252, Percent_Identity=40.0793650793651, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28217; Mature: 28086

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQPPRILAIAGSDSSGGAGIQADIKTITMLGGYAMTAITAVTAQNSVGVQGITPLDGA
CCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHH
FVGQQIASAIDDIGVDAIKIGMLQNEDIILAVANALEEIDPQVPIVLDPVMVATSGAPLV
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEECCCEEECCCCCEE
VQDAVATMRERLFTKARLLTPNLPELELLAGKQLRTTELVREAAEDLARHYGCDVLAKGG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCC
HTADERIIDVLVPVGGRMAQFDDARIDTRHTHGTGCTLSSAIATLLGHGQPMEHAVRLAR
CCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RFVREAIENAPGFGDGAGPLGHQAVRLKS
HHHHHHHHCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
SRQPPRILAIAGSDSSGGAGIQADIKTITMLGGYAMTAITAVTAQNSVGVQGITPLDGA
CCCCCEEEEEECCCCCCCCCEEEHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHH
FVGQQIASAIDDIGVDAIKIGMLQNEDIILAVANALEEIDPQVPIVLDPVMVATSGAPLV
HHHHHHHHHHHHCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCEEECCCEEECCCCCEE
VQDAVATMRERLFTKARLLTPNLPELELLAGKQLRTTELVREAAEDLARHYGCDVLAKGG
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCC
HTADERIIDVLVPVGGRMAQFDDARIDTRHTHGTGCTLSSAIATLLGHGQPMEHAVRLAR
CCHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RFVREAIENAPGFGDGAGPLGHQAVRLKS
HHHHHHHHCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA