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The map label for this gene is rlmE

Identifier: 85374883

GI number: 85374883

Start: 2087150

End: 2087812

Strand: Direct

Name: rlmE

Synonym: ELI_10280

Alternate gene names: 85374883

Gene position: 2087150-2087812 (Clockwise)

Preceding gene: 85374882

Following gene: 85374885

Centisome position: 68.38

GC content: 63.95

Gene sequence:

>663_bases
ATGAGCCGGTCCGGCAAAGATCCAGGCAAGCGGGTCAAGACGGCGCGCAAGAGATCGGCCTCCTCGACCCGTTGGCTCGA
GCGCCAGCTAAACGATCCCTATGTCAAGCAGGCCAAGGCCGACGGCTATCGCAGCCGCGCGGCCTACAAGCTGATCGAGC
TCGACGGGAAATTCGGCATCCTGAAAGGCGCGTCACGCGTCGTCGATCTCGGCATTGCGCCGGGCGGCTGGGCGCAGGTG
GTGCGCAAGGTGCGCCCGAAGGCAGGTATCGTCGGCATCGACCTGCTGGAAACCGAACCGATCGAAGGCGTCACCATCCT
GCAGATGGATTTCATGGCCGACGAAGCACCGGCCGGACTGGAGGCGGCTCTCGATGGTCCGCCCGACCTGGTGATGAGCG
ACATGGCTGCCAATACCGTCGGCCACAAGCAAACCGACCACCTGCGCACGATGGGGCTCGTCGAAGCCGCCGCATGGTTC
GCCATCGAGACGCTGGAGGAAGGCGGCACCTTCCTTGCCAAGGTCCTGGCCGGCGGGACCGACAATGATCTGCTCAAGCT
GCTCAAGAGCCATTTCAAGACCGTCAAGCATGCCAAGCCACCGGCGAGCCGCAAAGGCTCGTCGGAATGGTATGTCGTCG
CTCAGGGGTTCAAGGGGCGCTGA

Upstream 100 bases:

>100_bases
CCGATCGCGGCATCCGCGAAGGCATCCTGCGCAGCCTGATGGCGGGCGATTTCGCCACCGCGCGCGATCGCAAGGCGATG
GACGAAACCCGGAAAGCACA

Downstream 100 bases:

>100_bases
AACAATCGCTTCAGGACGCCGCAGCGATCAGGTACCCGGCGGATTTCCAGGCACCGGCTGCGCCATATCCATGGCTTCGG
CCGCTTCGACCGGATCGATC

Product: 23S rRNA methylase

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSRSGKDPGKRVKTARKRSASSTRWLERQLNDPYVKQAKADGYRSRAAYKLIELDGKFGILKGASRVVDLGIAPGGWAQV
VRKVRPKAGIVGIDLLETEPIEGVTILQMDFMADEAPAGLEAALDGPPDLVMSDMAANTVGHKQTDHLRTMGLVEAAAWF
AIETLEEGGTFLAKVLAGGTDNDLLKLLKSHFKTVKHAKPPASRKGSSEWYVVAQGFKGR

Sequences:

>Translated_220_residues
MSRSGKDPGKRVKTARKRSASSTRWLERQLNDPYVKQAKADGYRSRAAYKLIELDGKFGILKGASRVVDLGIAPGGWAQV
VRKVRPKAGIVGIDLLETEPIEGVTILQMDFMADEAPAGLEAALDGPPDLVMSDMAANTVGHKQTDHLRTMGLVEAAAWF
AIETLEEGGTFLAKVLAGGTDNDLLKLLKSHFKTVKHAKPPASRKGSSEWYVVAQGFKGR
>Mature_219_residues
SRSGKDPGKRVKTARKRSASSTRWLERQLNDPYVKQAKADGYRSRAAYKLIELDGKFGILKGASRVVDLGIAPGGWAQVV
RKVRPKAGIVGIDLLETEPIEGVTILQMDFMADEAPAGLEAALDGPPDLVMSDMAANTVGHKQTDHLRTMGLVEAAAWFA
IETLEEGGTFLAKVLAGGTDNDLLKLLKSHFKTVKHAKPPASRKGSSEWYVVAQGFKGR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=219, Percent_Identity=38.3561643835616, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=37.2340425531915, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=35.2631578947368, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1789569, Length=208, Percent_Identity=45.1923076923077, Blast_Score=148, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=211, Percent_Identity=35.5450236966825, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=36.7875647668394, Blast_Score=116, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=36.7875647668394, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71987550, Length=193, Percent_Identity=36.7875647668394, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554650, Length=195, Percent_Identity=36.9230769230769, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319796, Length=192, Percent_Identity=34.375, Blast_Score=108, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6321302, Length=103, Percent_Identity=44.6601941747573, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=34.1708542713568, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356387, Length=226, Percent_Identity=35.8407079646018, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=38.8297872340425, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=35.3535353535354, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=35.2040816326531, Blast_Score=107, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_ERYLH (Q2N843)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458945.1
- ProteinModelPortal:   Q2N843
- SMR:   Q2N843
- STRING:   Q2N843
- GeneID:   3869423
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_10280
- NMPDR:   fig|314225.3.peg.976
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q2N843
- ProtClustDB:   CLSK835143
- BioCyc:   ELIT314225:ELI_10280-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23813; Mature: 23681

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 75-75 BINDING 77-77 BINDING 94-94 BINDING 110-110 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSGKDPGKRVKTARKRSASSTRWLERQLNDPYVKQAKADGYRSRAAYKLIELDGKFGI
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHEEEEEECCCCCC
LKGASRVVDLGIAPGGWAQVVRKVRPKAGIVGIDLLETEPIEGVTILQMDFMADEAPAGL
HHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCC
EAALDGPPDLVMSDMAANTVGHKQTDHLRTMGLVEAAAWFAIETLEEGGTFLAKVLAGGT
EECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DNDLLKLLKSHFKTVKHAKPPASRKGSSEWYVVAQGFKGR
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SRSGKDPGKRVKTARKRSASSTRWLERQLNDPYVKQAKADGYRSRAAYKLIELDGKFGI
CCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHCCCHHHHHHEEEEEECCCCCC
LKGASRVVDLGIAPGGWAQVVRKVRPKAGIVGIDLLETEPIEGVTILQMDFMADEAPAGL
HHCCCCEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCC
EAALDGPPDLVMSDMAANTVGHKQTDHLRTMGLVEAAAWFAIETLEEGGTFLAKVLAGGT
EECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DNDLLKLLKSHFKTVKHAKPPASRKGSSEWYVVAQGFKGR
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA