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The map label for this gene is gcvT

Identifier: 85374842

GI number: 85374842

Start: 2044322

End: 2045461

Strand: Reverse

Name: gcvT

Synonym: ELI_10075

Alternate gene names: 85374842

Gene position: 2045461-2044322 (Counterclockwise)

Preceding gene: 85374843

Following gene: 85374841

Centisome position: 67.01

GC content: 64.04

Gene sequence:

>1140_bases
ATGAGCGATACCGACACCCAGGATTTGGCCAAGCTGCCGCTCGATGCATGGCATCGCGCGCAGGGCGCCCGCATGGTGCC
GTTCGCCGGCTACGAAATGCCGATCCAGTATACCGGCCCGTCCGGCGGCATCGTCGCGGAGCATAATTGGACGCGCGAAA
GTGCCAGCCTGTTCGATGTCAGCCACATGGGCCAGCTGGTCATCAGCGGCGAAGGCGCGACCGAGGCGCTCGAGGCGCTC
GTTCCCGGCAAGCTGTCGTCGCTGAAACCCGGCAATATCCGCTACACGCTGCTGCTGAACGAAGACGGCGGAATTCTCGA
CGACCTGATGGTCACCAACATGTCGCGCGAGGATGTCGAAGAGTTCTACCTCGTCGTGAACGGAGCGACCAAGTGGGACG
ATATCGCCCACCTTCGCGAACACCTGCCCGACGAAATCACGATGAACCATCTCGATGGGCGCGGCCTTGTGGCGCTGCAG
GGGCCGAAGGCTGCCGAAGCGCTCGAGACGGTCTTTCCGGGCGTCGCGGAGCGGCTCGTTTTCATGCAGGGCACGGGCTT
CGAGTGGAGGGGCGACATGATCGGCGTCGCGCGCTGCGGCTATACCGGCGAGGACGGCTTCGAGATCAATATCTCGGCCA
GCCATATCGAACAGCTGGCCGAAGCGCTGGTGGCCGACGATCGGGTCAGGCCTGCCGGGCTCGGCGCACGCGACAGCTTG
CGTTTGGAGGCAGGCCTGCCGCTCTACGGCCACGATCTCACGGTCGAGACCGATCCCGTCAGCGCCGACCTGACTTTTGC
CCTCACCAAGTCGCGCCGCGAGACCGGCGGGTGGATGGGCCACGGACGCATAGCGCAAGCGCTTATGAGCGGTCCCGAAA
CCAAGCGCGTCGGCCTGAAGATCGAAGGCCGTATGCCTGCCCGCGAAGGTGCCTTGGTCTATATCGGCGATGCCGAAATC
GGCCGCGTTACCAGTGGCGGCTTCTCGCCCACACTCGGCTATCCGATCGCCATGGCCTATATCGCACGAGAGCAGGCCAT
CGAAGGCACCGAGGTCGAAATCGAAGTTCGCAACAAGCGTCTGCCCGCGACCGTCGTGCCGATGCCGTTCGTACCCCACC
GCTACTATCGAGGGAAATAG

Upstream 100 bases:

>100_bases
GGAAGTGCGGATCACCCGGCCCGCACGATGCTCTCAGGTTTCCCGACAGAGGGGGCGGAACAGCGCTTCACTTCGAAGCG
TTGCTGTGCCGGGGAATTTG

Downstream 100 bases:

>100_bases
AGAGCATGGCCCGTTACTTTACCGATGAACATGAATGGATCGATCTCGAAGGCGACCTCGCGACGGTCGGCATCACCGAT
TACGCGCAGGAGCAGCTGGG

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSDTDTQDLAKLPLDAWHRAQGARMVPFAGYEMPIQYTGPSGGIVAEHNWTRESASLFDVSHMGQLVISGEGATEALEAL
VPGKLSSLKPGNIRYTLLLNEDGGILDDLMVTNMSREDVEEFYLVVNGATKWDDIAHLREHLPDEITMNHLDGRGLVALQ
GPKAAEALETVFPGVAERLVFMQGTGFEWRGDMIGVARCGYTGEDGFEINISASHIEQLAEALVADDRVRPAGLGARDSL
RLEAGLPLYGHDLTVETDPVSADLTFALTKSRRETGGWMGHGRIAQALMSGPETKRVGLKIEGRMPAREGALVYIGDAEI
GRVTSGGFSPTLGYPIAMAYIAREQAIEGTEVEIEVRNKRLPATVVPMPFVPHRYYRGK

Sequences:

>Translated_379_residues
MSDTDTQDLAKLPLDAWHRAQGARMVPFAGYEMPIQYTGPSGGIVAEHNWTRESASLFDVSHMGQLVISGEGATEALEAL
VPGKLSSLKPGNIRYTLLLNEDGGILDDLMVTNMSREDVEEFYLVVNGATKWDDIAHLREHLPDEITMNHLDGRGLVALQ
GPKAAEALETVFPGVAERLVFMQGTGFEWRGDMIGVARCGYTGEDGFEINISASHIEQLAEALVADDRVRPAGLGARDSL
RLEAGLPLYGHDLTVETDPVSADLTFALTKSRRETGGWMGHGRIAQALMSGPETKRVGLKIEGRMPAREGALVYIGDAEI
GRVTSGGFSPTLGYPIAMAYIAREQAIEGTEVEIEVRNKRLPATVVPMPFVPHRYYRGK
>Mature_378_residues
SDTDTQDLAKLPLDAWHRAQGARMVPFAGYEMPIQYTGPSGGIVAEHNWTRESASLFDVSHMGQLVISGEGATEALEALV
PGKLSSLKPGNIRYTLLLNEDGGILDDLMVTNMSREDVEEFYLVVNGATKWDDIAHLREHLPDEITMNHLDGRGLVALQG
PKAAEALETVFPGVAERLVFMQGTGFEWRGDMIGVARCGYTGEDGFEINISASHIEQLAEALVADDRVRPAGLGARDSLR
LEAGLPLYGHDLTVETDPVSADLTFALTKSRRETGGWMGHGRIAQALMSGPETKRVGLKIEGRMPAREGALVYIGDAEIG
RVTSGGFSPTLGYPIAMAYIAREQAIEGTEVEIEVRNKRLPATVVPMPFVPHRYYRGK

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=379, Percent_Identity=39.8416886543536, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI257796258, Length=355, Percent_Identity=38.3098591549296, Blast_Score=208, Evalue=5e-54,
Organism=Homo sapiens, GI257796254, Length=372, Percent_Identity=35.4838709677419, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI257796256, Length=324, Percent_Identity=38.8888888888889, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI197927446, Length=310, Percent_Identity=24.5161290322581, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI21361378, Length=310, Percent_Identity=24.5161290322581, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI24797151, Length=340, Percent_Identity=22.9411764705882, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789272, Length=369, Percent_Identity=35.230352303523, Blast_Score=168, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17560118, Length=375, Percent_Identity=40.2666666666667, Blast_Score=233, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6320222, Length=388, Percent_Identity=39.6907216494845, Blast_Score=252, Evalue=8e-68,
Organism=Drosophila melanogaster, GI20129441, Length=373, Percent_Identity=39.6782841823056, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI20130091, Length=349, Percent_Identity=22.9226361031519, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 41244; Mature: 41113

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTDTQDLAKLPLDAWHRAQGARMVPFAGYEMPIQYTGPSGGIVAEHNWTRESASLFDV
CCCCCCHHHHHCCHHHHHHCCCCEECEECCCCCEEEEECCCCCEEECCCCCCCCCCEEEH
SHMGQLVISGEGATEALEALVPGKLSSLKPGNIRYTLLLNEDGGILDDLMVTNMSREDVE
HHCCEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHE
EFYLVVNGATKWDDIAHLREHLPDEITMNHLDGRGLVALQGPKAAEALETVFPGVAERLV
EEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEE
FMQGTGFEWRGDMIGVARCGYTGEDGFEINISASHIEQLAEALVADDRVRPAGLGARDSL
EEECCCCEECCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCE
RLEAGLPLYGHDLTVETDPVSADLTFALTKSRRETGGWMGHGRIAQALMSGPETKRVGLK
EEECCCCEECCCEEEECCCCCCCEEEEEECCCHHCCCCCCCHHHHHHHHCCCCCEEEEEE
IEGRMPAREGALVYIGDAEIGRVTSGGFSPTLGYPIAMAYIAREQAIEGTEVEIEVRNKR
EECCCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
LPATVVPMPFVPHRYYRGK
CCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SDTDTQDLAKLPLDAWHRAQGARMVPFAGYEMPIQYTGPSGGIVAEHNWTRESASLFDV
CCCCCHHHHHCCHHHHHHCCCCEECEECCCCCEEEEECCCCCEEECCCCCCCCCCEEEH
SHMGQLVISGEGATEALEALVPGKLSSLKPGNIRYTLLLNEDGGILDDLMVTNMSREDVE
HHCCEEEEECCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCHHHHE
EFYLVVNGATKWDDIAHLREHLPDEITMNHLDGRGLVALQGPKAAEALETVFPGVAERLV
EEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEE
FMQGTGFEWRGDMIGVARCGYTGEDGFEINISASHIEQLAEALVADDRVRPAGLGARDSL
EEECCCCEECCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCE
RLEAGLPLYGHDLTVETDPVSADLTFALTKSRRETGGWMGHGRIAQALMSGPETKRVGLK
EEECCCCEECCCEEEECCCCCCCEEEEEECCCHHCCCCCCCHHHHHHHHCCCCCEEEEEE
IEGRMPAREGALVYIGDAEIGRVTSGGFSPTLGYPIAMAYIAREQAIEGTEVEIEVRNKR
EECCCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
LPATVVPMPFVPHRYYRGK
CCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA