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The map label for this gene is metH [H]

Identifier: 85374843

GI number: 85374843

Start: 2045760

End: 2048387

Strand: Reverse

Name: metH [H]

Synonym: ELI_10080

Alternate gene names: 85374843

Gene position: 2048387-2045760 (Counterclockwise)

Preceding gene: 85374844

Following gene: 85374842

Centisome position: 67.11

GC content: 62.14

Gene sequence:

>2628_bases
ATGAGCGATCGCAATACCGCCCGCTTCGTCAATATCGGCGAGCGCACCAATGTGACCGGGTCGGCCCGGTTCAAGAAGCT
CATCATGGCGGACGATTACGCGACCGCCGTCGACGTCGCGCGCCAGCAGGTCGAGAACGGCGCGCAGGTGATCGACGTCA
ACATGGACGAGGGCCTGCTCGACGCGGTTCACGCCATGACCACCTTCCTCAAGCTGATTGCCGCCGAACCCGATATCGCC
CGGGTGCCGGTGATGATCGACAGTTCCAAATGGGATGTCATCGAGGCGGGCCTGAAATGCGTCTCCGGCAAGCCGATCGT
CAATTCGATCAGCATGAAGGAAGGCGAGGAGGAGTTTCTCGATCACGCGCGCAAATGCATGGACTATGGCGCGGCTGCGG
TCGTCATGGCCTTCGACGAGACCGGCCAGGCGGACACCAAGCAGCGCAAGGTCGAGATCTGCACCCGCGCTTATAACCTG
CTGACAAGCATCGGCTTTCCGCCCGAAGACATCATTTTCGACCCCAATATCTTCGCTGTGGCGACCGGCATCGAAGAGCA
TGATCGCTACGGGCTGGACTTCATCGAGGCCGTTTCCGAGATTAAGGCGGCGTGTCCGCATGCCAAGACCAGCGGCGGAC
TGTCCAACCTGTCCTTCAGTTTCCGCGGAAACGAAACCGTGCGCCGCGCGATGCACTCGGTGTTCCTCTATCACGCGATT
CCCGCCGGGCTCGACATGGCCATCGTCAACGCCGGCCAGCTCGATATCTACGACGATATCGAACCTGCATTGCGCGAGGC
GTGCGAGGATGTGATCCTTTGTCTTCGGCCCGATGCGACCGAGCGGCTGATCGATCTGGCGGAAAGCTACAAGGGCAGGT
CCGTCGCCGACGAAAAGGCGGCCGAGGAATGGCGCGGCTGGGCGGTCGAGAAACGGCTCGAACACGCGCTGGTCAAGGGC
ATCGACGCGCATGTTGTCGACGATACCGAGGAAATGCGTGCGCTCACCTCAGAGCAGGGCGGTCGCCCGATCGAAGTCAT
CGAAGGCCCTTTGATGGACGGCATGAATGTCGTCGGCGACCTGTTCGGCAGCGGCAAGATGTTCCTGCCTCAGGTCGTGA
AGTCCGCGCGCGTGATGAAGAAGGCGGTCGCACACCTCATCCCGTTTATCGAGGCGGAGAAAGACCTGCTGCCCGAGGCC
GAGCGCAAGGCCAAGGGCAAGGTCATCATGGCAACCGTCAAGGGCGATGTTCACGATATCGGCAAGAACATCGTCGGCGT
CGTCCTGCAGTGCAACGGATACGACGTGGTCGATATGGGCGTGATGGTCCCGTGGCAGGACATTCTCAAATCCGCGAACG
ACAACAAGGCCGACATGATTGGCCTGTCCGGCCTTATCACCCCTTCGCTGGACGAGATGGTGAGCGTGGCCGAGGAGATG
CAGCGCGCGGGAATGACCATGCCGCTGCTGATCGGTGGCGCGACCACCAGCAAGGTTCACACCGCGCTCAAGATCGATCC
CGCCTATGAGGGCCCTGTCATTCACGTGCTCGACGCGAGCCGCGCCGTCGGTGTCGCCAGTCGGCTGCTGTCCGACACCC
AGCGCGACGAATTCGTCGAGACGACCGCCAGCGATTACCAGCACGTCCGCGATGTGCGCGAAGGGAAGGGGCAGAGCGCG
CTCTTCACCATCGAGGAGGCACGGGCGAATTTCTACGACGCCTATCTCAGCGACAAGCCTGCGCCGCCGCTCCAGCCGGG
AGTGCATGCTTTCGACGAATGGTCTCTCGCCGACCTTCGGGAGTATATCGACTGGACGCCGTTCTTCCGCGCTTGGGAAC
TGCACGGCACCTATCCGCGCATACTCGACGACGAAGTGGTGGGCGAAACTGCACGCAGCCTGTTCGCGGATGCGCAGCAG
ATGCTGGACCATATCGTCGAGGAGAATTGGCTGACGGCGAAGGGCGTGGCCGGGCTCTGGCCATGCGCCCGTGACGGCGA
CGATGTGACGATCCACCTTGCCGAGGAGGAAGAGCATGTCCTGCTCCCCTTCCTGCGCCAACAGGTGAAGAAGAGCCGCG
ACCGGGCGAATTTCTGCCTCGCCGATTTCATCGATCCCGCCGGCGACTGGATTGGCGGATTCGCGGTCGGCATCCACGGG
ATCGAACCGCATAGCGCGCGCTTCCAGGCCGACAAGGACGATTACTCGGACATCCTGCTCAAGGCGCTGGCCGATCGCTT
CGCCGAGGCCTTTGCCGAGCGCCTGCACCAGCATACCCGCACCGATTTGTGGGGGTATGCACCGAACGAGCAGCTCACCA
ACGAGGCGCTGATCAAGGAGCAGTATCGCGGCATTCGCCCGGCGCCAGGCTATCCGGCGTGCCCCGATCATTCGCTCAAG
CCGATCCTGTTCGACCTGCTCGACGCCGAGAAGAACGTTGGCCTGACTTTGACCGAAAGCTTCGCGATGTATCCGACAGC
GGCTGTCAGCGGCTTCTACTTCGGGCATCCCGAAAGCCAATATTTCGGCGTCGCCAGGATCGGCCGCGACCAGCTGGAAG
ATTACGCGCAGCGTCGCGGTGTGGATATCGCGACTGCCGAACGGTGGCTGAGGCCCAATCTCGATTGA

Upstream 100 bases:

>100_bases
TCTACGCCAAGCAAGGCTTCCGCCAGATCGGCACCCGTCGTTTCGATGTCGGCGGCAAGCATTACGACGATGTCGTCCTC
GCGCGCGATCTGGTGACGGC

Downstream 100 bases:

>100_bases
CCGGCCCCGCCGTTTCGCGCATGCAGCATCCGTCTTTCGGCGGGAGCGATTCTGCCGGACGGACCAGCGGGAGAGTTCGC
GCTTCGCGGCGCGACGCCGA

Product: 5-methyltetrahydrofolate-homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MSDRNTARFVNIGERTNVTGSARFKKLIMADDYATAVDVARQQVENGAQVIDVNMDEGLLDAVHAMTTFLKLIAAEPDIA
RVPVMIDSSKWDVIEAGLKCVSGKPIVNSISMKEGEEEFLDHARKCMDYGAAAVVMAFDETGQADTKQRKVEICTRAYNL
LTSIGFPPEDIIFDPNIFAVATGIEEHDRYGLDFIEAVSEIKAACPHAKTSGGLSNLSFSFRGNETVRRAMHSVFLYHAI
PAGLDMAIVNAGQLDIYDDIEPALREACEDVILCLRPDATERLIDLAESYKGRSVADEKAAEEWRGWAVEKRLEHALVKG
IDAHVVDDTEEMRALTSEQGGRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAEKDLLPEA
ERKAKGKVIMATVKGDVHDIGKNIVGVVLQCNGYDVVDMGVMVPWQDILKSANDNKADMIGLSGLITPSLDEMVSVAEEM
QRAGMTMPLLIGGATTSKVHTALKIDPAYEGPVIHVLDASRAVGVASRLLSDTQRDEFVETTASDYQHVRDVREGKGQSA
LFTIEEARANFYDAYLSDKPAPPLQPGVHAFDEWSLADLREYIDWTPFFRAWELHGTYPRILDDEVVGETARSLFADAQQ
MLDHIVEENWLTAKGVAGLWPCARDGDDVTIHLAEEEEHVLLPFLRQQVKKSRDRANFCLADFIDPAGDWIGGFAVGIHG
IEPHSARFQADKDDYSDILLKALADRFAEAFAERLHQHTRTDLWGYAPNEQLTNEALIKEQYRGIRPAPGYPACPDHSLK
PILFDLLDAEKNVGLTLTESFAMYPTAAVSGFYFGHPESQYFGVARIGRDQLEDYAQRRGVDIATAERWLRPNLD

Sequences:

>Translated_875_residues
MSDRNTARFVNIGERTNVTGSARFKKLIMADDYATAVDVARQQVENGAQVIDVNMDEGLLDAVHAMTTFLKLIAAEPDIA
RVPVMIDSSKWDVIEAGLKCVSGKPIVNSISMKEGEEEFLDHARKCMDYGAAAVVMAFDETGQADTKQRKVEICTRAYNL
LTSIGFPPEDIIFDPNIFAVATGIEEHDRYGLDFIEAVSEIKAACPHAKTSGGLSNLSFSFRGNETVRRAMHSVFLYHAI
PAGLDMAIVNAGQLDIYDDIEPALREACEDVILCLRPDATERLIDLAESYKGRSVADEKAAEEWRGWAVEKRLEHALVKG
IDAHVVDDTEEMRALTSEQGGRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAEKDLLPEA
ERKAKGKVIMATVKGDVHDIGKNIVGVVLQCNGYDVVDMGVMVPWQDILKSANDNKADMIGLSGLITPSLDEMVSVAEEM
QRAGMTMPLLIGGATTSKVHTALKIDPAYEGPVIHVLDASRAVGVASRLLSDTQRDEFVETTASDYQHVRDVREGKGQSA
LFTIEEARANFYDAYLSDKPAPPLQPGVHAFDEWSLADLREYIDWTPFFRAWELHGTYPRILDDEVVGETARSLFADAQQ
MLDHIVEENWLTAKGVAGLWPCARDGDDVTIHLAEEEEHVLLPFLRQQVKKSRDRANFCLADFIDPAGDWIGGFAVGIHG
IEPHSARFQADKDDYSDILLKALADRFAEAFAERLHQHTRTDLWGYAPNEQLTNEALIKEQYRGIRPAPGYPACPDHSLK
PILFDLLDAEKNVGLTLTESFAMYPTAAVSGFYFGHPESQYFGVARIGRDQLEDYAQRRGVDIATAERWLRPNLD
>Mature_874_residues
SDRNTARFVNIGERTNVTGSARFKKLIMADDYATAVDVARQQVENGAQVIDVNMDEGLLDAVHAMTTFLKLIAAEPDIAR
VPVMIDSSKWDVIEAGLKCVSGKPIVNSISMKEGEEEFLDHARKCMDYGAAAVVMAFDETGQADTKQRKVEICTRAYNLL
TSIGFPPEDIIFDPNIFAVATGIEEHDRYGLDFIEAVSEIKAACPHAKTSGGLSNLSFSFRGNETVRRAMHSVFLYHAIP
AGLDMAIVNAGQLDIYDDIEPALREACEDVILCLRPDATERLIDLAESYKGRSVADEKAAEEWRGWAVEKRLEHALVKGI
DAHVVDDTEEMRALTSEQGGRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAEKDLLPEAE
RKAKGKVIMATVKGDVHDIGKNIVGVVLQCNGYDVVDMGVMVPWQDILKSANDNKADMIGLSGLITPSLDEMVSVAEEMQ
RAGMTMPLLIGGATTSKVHTALKIDPAYEGPVIHVLDASRAVGVASRLLSDTQRDEFVETTASDYQHVRDVREGKGQSAL
FTIEEARANFYDAYLSDKPAPPLQPGVHAFDEWSLADLREYIDWTPFFRAWELHGTYPRILDDEVVGETARSLFADAQQM
LDHIVEENWLTAKGVAGLWPCARDGDDVTIHLAEEEEHVLLPFLRQQVKKSRDRANFCLADFIDPAGDWIGGFAVGIHGI
EPHSARFQADKDDYSDILLKALADRFAEAFAERLHQHTRTDLWGYAPNEQLTNEALIKEQYRGIRPAPGYPACPDHSLKP
ILFDLLDAEKNVGLTLTESFAMYPTAAVSGFYFGHPESQYFGVARIGRDQLEDYAQRRGVDIATAERWLRPNLD

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=892, Percent_Identity=52.8026905829596, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=881, Percent_Identity=61.5209988649262, Blast_Score=1062, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=890, Percent_Identity=52.0224719101124, Blast_Score=868, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 96827; Mature: 96695

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRNTARFVNIGERTNVTGSARFKKLIMADDYATAVDVARQQVENGAQVIDVNMDEGLL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
DAVHAMTTFLKLIAAEPDIARVPVMIDSSKWDVIEAGLKCVSGKPIVNSISMKEGEEEFL
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCHHHHH
DHARKCMDYGAAAVVMAFDETGQADTKQRKVEICTRAYNLLTSIGFPPEDIIFDPNIFAV
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCEECCCCEEE
ATGIEEHDRYGLDFIEAVSEIKAACPHAKTSGGLSNLSFSFRGNETVRRAMHSVFLYHAI
ECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
PAGLDMAIVNAGQLDIYDDIEPALREACEDVILCLRPDATERLIDLAESYKGRSVADEKA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCCHHHHH
AEEWRGWAVEKRLEHALVKGIDAHVVDDTEEMRALTSEQGGRPIEVIEGPLMDGMNVVGD
HHHHHCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHH
LFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAEKDLLPEAERKAKGKVIMATVKGDVHDI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHCCCCEEEEEECCCHHHH
GKNIVGVVLQCNGYDVVDMGVMVPWQDILKSANDNKADMIGLSGLITPSLDEMVSVAEEM
CCHHEEEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QRAGMTMPLLIGGATTSKVHTALKIDPAYEGPVIHVLDASRAVGVASRLLSDTQRDEFVE
HHCCCCEEEEEECCCHHHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
TTASDYQHVRDVREGKGQSALFTIEEARANFYDAYLSDKPAPPLQPGVHAFDEWSLADLR
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
EYIDWTPFFRAWELHGTYPRILDDEVVGETARSLFADAQQMLDHIVEENWLTAKGVAGLW
HHHCCCHHHHEEECCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PCARDGDDVTIHLAEEEEHVLLPFLRQQVKKSRDRANFCLADFIDPAGDWIGGFAVGIHG
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCCHHEEEEC
IEPHSARFQADKDDYSDILLKALADRFAEAFAERLHQHTRTDLWGYAPNEQLTNEALIKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
QYRGIRPAPGYPACPDHSLKPILFDLLDAEKNVGLTLTESFAMYPTAAVSGFYFGHPESQ
HHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHCCHHHHCCCCCCCCCCC
YFGVARIGRDQLEDYAQRRGVDIATAERWLRPNLD
CHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
SDRNTARFVNIGERTNVTGSARFKKLIMADDYATAVDVARQQVENGAQVIDVNMDEGLL
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
DAVHAMTTFLKLIAAEPDIARVPVMIDSSKWDVIEAGLKCVSGKPIVNSISMKEGEEEFL
HHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCHHHHH
DHARKCMDYGAAAVVMAFDETGQADTKQRKVEICTRAYNLLTSIGFPPEDIIFDPNIFAV
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCEECCCCEEE
ATGIEEHDRYGLDFIEAVSEIKAACPHAKTSGGLSNLSFSFRGNETVRRAMHSVFLYHAI
ECCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
PAGLDMAIVNAGQLDIYDDIEPALREACEDVILCLRPDATERLIDLAESYKGRSVADEKA
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCCHHHHH
AEEWRGWAVEKRLEHALVKGIDAHVVDDTEEMRALTSEQGGRPIEVIEGPLMDGMNVVGD
HHHHHCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHH
LFGSGKMFLPQVVKSARVMKKAVAHLIPFIEAEKDLLPEAERKAKGKVIMATVKGDVHDI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHCCCCEEEEEECCCHHHH
GKNIVGVVLQCNGYDVVDMGVMVPWQDILKSANDNKADMIGLSGLITPSLDEMVSVAEEM
CCHHEEEEEEECCCCEEECCCCCCHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
QRAGMTMPLLIGGATTSKVHTALKIDPAYEGPVIHVLDASRAVGVASRLLSDTQRDEFVE
HHCCCCEEEEEECCCHHHHEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
TTASDYQHVRDVREGKGQSALFTIEEARANFYDAYLSDKPAPPLQPGVHAFDEWSLADLR
HHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
EYIDWTPFFRAWELHGTYPRILDDEVVGETARSLFADAQQMLDHIVEENWLTAKGVAGLW
HHHCCCHHHHEEECCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PCARDGDDVTIHLAEEEEHVLLPFLRQQVKKSRDRANFCLADFIDPAGDWIGGFAVGIHG
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCCHHEEEEC
IEPHSARFQADKDDYSDILLKALADRFAEAFAERLHQHTRTDLWGYAPNEQLTNEALIKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
QYRGIRPAPGYPACPDHSLKPILFDLLDAEKNVGLTLTESFAMYPTAAVSGFYFGHPESQ
HHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCHHHCCHHHHCCCCCCCCCCC
YFGVARIGRDQLEDYAQRRGVDIATAERWLRPNLD
CHHHHHHCHHHHHHHHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185137; 2668277; 8369296; 8265357; 9278503; 1593636; 7992050; 8939751 [H]