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The map label for this gene is gcvH [H]

Identifier: 85374841

GI number: 85374841

Start: 2043945

End: 2044316

Strand: Reverse

Name: gcvH [H]

Synonym: ELI_10070

Alternate gene names: 85374841

Gene position: 2044316-2043945 (Counterclockwise)

Preceding gene: 85374842

Following gene: 85374840

Centisome position: 66.97

GC content: 60.48

Gene sequence:

>372_bases
ATGGCCCGTTACTTTACCGATGAACATGAATGGATCGATCTCGAAGGCGACCTCGCGACGGTCGGCATCACCGATTACGC
GCAGGAGCAGCTGGGCGATATCGTCTTCGTCGAGCTGCCCGAGGTCGGCAGTAATGTCGACAAGGGGGGGGAAGCGGCGG
TCGTAGAGAGCGTCAAGGCCGCGAGCGACGTCTACGCGCCGATCACCGGCGAGGTGATGGAAGCCAATGCCGCGCTGGAA
GAAGACCCCGCGCTGGTGAACACCTCGCCGGAGGAGGAAGGCTGGTTTTTCCGCATGACCGTCGCCGACAAATCCGAACT
CGATGGGCTGATGGACGCGAAAGCCTACAAGGCATTCTGCGACGAACTTTAA

Upstream 100 bases:

>100_bases
GCACCGAGGTCGAAATCGAAGTTCGCAACAAGCGTCTGCCCGCGACCGTCGTGCCGATGCCGTTCGTACCCCACCGCTAC
TATCGAGGGAAATAGAGAGC

Downstream 100 bases:

>100_bases
CCGTTATCCCAGCGCAAGCTGGGATATCTGTCCGCCTTATCGGACATCACTGCAACAGATCCCAGCGTCCGCTGGGATGA
CGAGATGGCACCATGCGTTA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MARYFTDEHEWIDLEGDLATVGITDYAQEQLGDIVFVELPEVGSNVDKGGEAAVVESVKAASDVYAPITGEVMEANAALE
EDPALVNTSPEEEGWFFRMTVADKSELDGLMDAKAYKAFCDEL

Sequences:

>Translated_123_residues
MARYFTDEHEWIDLEGDLATVGITDYAQEQLGDIVFVELPEVGSNVDKGGEAAVVESVKAASDVYAPITGEVMEANAALE
EDPALVNTSPEEEGWFFRMTVADKSELDGLMDAKAYKAFCDEL
>Mature_122_residues
ARYFTDEHEWIDLEGDLATVGITDYAQEQLGDIVFVELPEVGSNVDKGGEAAVVESVKAASDVYAPITGEVMEANAALEE
DPALVNTSPEEEGWFFRMTVADKSELDGLMDAKAYKAFCDEL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=51.2820512820513, Blast_Score=133, Evalue=3e-32,
Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=51.2820512820513, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789271, Length=119, Percent_Identity=52.9411764705882, Blast_Score=130, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=121, Percent_Identity=47.1074380165289, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=49.1525423728814, Blast_Score=132, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6319272, Length=119, Percent_Identity=42.8571428571429, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=49.1525423728814, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13414; Mature: 13283

Theoretical pI: Translated: 3.63; Mature: 3.63

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYFTDEHEWIDLEGDLATVGITDYAQEQLGDIVFVELPEVGSNVDKGGEAAVVESVKA
CCCCCCCCCCEEECCCCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHH
ASDVYAPITGEVMEANAALEEDPALVNTSPEEEGWFFRMTVADKSELDGLMDAKAYKAFC
HHHCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
DEL
HCC
>Mature Secondary Structure 
ARYFTDEHEWIDLEGDLATVGITDYAQEQLGDIVFVELPEVGSNVDKGGEAAVVESVKA
CCCCCCCCCEEECCCCEEEECCCHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHH
ASDVYAPITGEVMEANAALEEDPALVNTSPEEEGWFFRMTVADKSELDGLMDAKAYKAFC
HHHCCCCCCCCEEECCCCCCCCCCEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
DEL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA