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The map label for this gene is dinB2 [H]

Identifier: 85374810

GI number: 85374810

Start: 2015366

End: 2016514

Strand: Direct

Name: dinB2 [H]

Synonym: ELI_09915

Alternate gene names: 85374810

Gene position: 2015366-2016514 (Clockwise)

Preceding gene: 85374808

Following gene: 85374815

Centisome position: 66.03

GC content: 65.8

Gene sequence:

>1149_bases
ATGGCGGCAGCGATGGCCGAGGACGATATCGACGACTCCAGCGACGCAGACGGAGAGGCAGATGGCCTGCGCAAGATCAT
CCATGTCGATATGGATGCCTTCTTCGCCAGCGTCGAACAGCGCGACAACCCCGACCTTCGGGGCAAGCCGGTGGCGGTCG
GCGGATCGAGCGGGCGCGGCGTGGTTGCCGCCGCCAGTTACGAAGCGCGCAGGTTCGGCGTGAAAAGCGCCATGCCGAGC
GTCACGGCGAAGCGGCTCTGCCCCGACCTGATTTTCGTGAAGCACCGCTTCGAGGCCTACAGGGAAGCGTCGGCCCGGAT
CCGCGAGATTTTCAATCGCTACACCCCGCATGTCGAACCGCTGAGCCTCGACGAGGCCTATCTCGACGTGACCGACGACC
TGCAGGCTGTCGGCTCGGCGACCGGGACGGCGCAGGCGATCCGCAAGGCTATCCGGGAAGAAACGGGCCTCACCGCCAGC
GCCGGGGTCAGCTACAACAAGTTTCTCGCCAAGCTCGCAAGCGACCAGAACAAGCCCGACGGTCTGTGCGTCATTAGGCC
CGGCGAAGGCGCGGCCTTCGTGCAGTCGCTGCCGATCCGGCGCTTTCACGGCGTCGGCCCGAAGGGCGCGGAGAAGATGG
CGCGGCTGGGGATCGACACCGGCGCGGACCTGGCCGGCAAGGACCTCGCCTGGCTGCGGCAGAATTTCGGCAGTTTCGGC
GATTACCTCTATCGCGCGGCGCGCGGGATCGACCTTCGCCCCATTCGCACCAACCGCTCTCGAAAATCGGTCGGCGGCGA
GCGGACGTTCAGCCAGGATCAGCACGAGCCCGACGAGTTGCGGGAAACGCTCGAACGGATAATCGCCATCGTGTGGGAAC
GGATCGAGCGGTCCAAGGCACGCGGACGCACGATCACGCTGAAGATGAAGTACAACGACTTCCGCACCATCACCCGCGCG
CAATCGCTGCCGCATTATGTGTCCGGGCAGGACGAGTTCGCATCCGTGGCGCGTGATCTGCTGGAGGCGCAGCTTCCGCT
GCCGCTGCCGATCAGGCTGATGGGGCTGACGCTCTCCAACCTCGAGCGCGACGAGGAAGCGCAGGCCCAGCGCAGCGAGC
GCAAGACCGGTCAACTGGCGCTGTTCTAG

Upstream 100 bases:

>100_bases
CGGATCGGGTTTTCGGGTCGGTTGCGGCCATGCAGGTCCCTTGCGCTGCGACCGGGCACTTTGCCAGCCATTTTTCTGAA
CATGCGGGCCAAGCCGCGCT

Downstream 100 bases:

>100_bases
ACCCGCGTCAGCGGGTGGACGCGCGCCACGCCTCGATCCGGCGACGCGTATTCTCGATCATCGGTTCGGGCAGCGAGTGG
CTGGGAAAGAACCGCGCCTC

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV 2 [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MAAAMAEDDIDDSSDADGEADGLRKIIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARRFGVKSAMPS
VTAKRLCPDLIFVKHRFEAYREASARIREIFNRYTPHVEPLSLDEAYLDVTDDLQAVGSATGTAQAIRKAIREETGLTAS
AGVSYNKFLAKLASDQNKPDGLCVIRPGEGAAFVQSLPIRRFHGVGPKGAEKMARLGIDTGADLAGKDLAWLRQNFGSFG
DYLYRAARGIDLRPIRTNRSRKSVGGERTFSQDQHEPDELRETLERIIAIVWERIERSKARGRTITLKMKYNDFRTITRA
QSLPHYVSGQDEFASVARDLLEAQLPLPLPIRLMGLTLSNLERDEEAQAQRSERKTGQLALF

Sequences:

>Translated_382_residues
MAAAMAEDDIDDSSDADGEADGLRKIIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARRFGVKSAMPS
VTAKRLCPDLIFVKHRFEAYREASARIREIFNRYTPHVEPLSLDEAYLDVTDDLQAVGSATGTAQAIRKAIREETGLTAS
AGVSYNKFLAKLASDQNKPDGLCVIRPGEGAAFVQSLPIRRFHGVGPKGAEKMARLGIDTGADLAGKDLAWLRQNFGSFG
DYLYRAARGIDLRPIRTNRSRKSVGGERTFSQDQHEPDELRETLERIIAIVWERIERSKARGRTITLKMKYNDFRTITRA
QSLPHYVSGQDEFASVARDLLEAQLPLPLPIRLMGLTLSNLERDEEAQAQRSERKTGQLALF
>Mature_381_residues
AAAMAEDDIDDSSDADGEADGLRKIIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRGVVAAASYEARRFGVKSAMPSV
TAKRLCPDLIFVKHRFEAYREASARIREIFNRYTPHVEPLSLDEAYLDVTDDLQAVGSATGTAQAIRKAIREETGLTASA
GVSYNKFLAKLASDQNKPDGLCVIRPGEGAAFVQSLPIRRFHGVGPKGAEKMARLGIDTGADLAGKDLAWLRQNFGSFGD
YLYRAARGIDLRPIRTNRSRKSVGGERTFSQDQHEPDELRETLERIIAIVWERIERSKARGRTITLKMKYNDFRTITRAQ
SLPHYVSGQDEFASVARDLLEAQLPLPLPIRLMGLTLSNLERDEEAQAQRSERKTGQLALF

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI84043967, Length=338, Percent_Identity=33.1360946745562, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI7706681, Length=339, Percent_Identity=33.0383480825959, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI154350220, Length=421, Percent_Identity=26.8408551068884, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI7705344, Length=110, Percent_Identity=51.8181818181818, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI5729982, Length=321, Percent_Identity=29.9065420560748, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786425, Length=309, Percent_Identity=50.1618122977346, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1787432, Length=230, Percent_Identity=32.1739130434783, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193205700, Length=409, Percent_Identity=31.7848410757946, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537959, Length=321, Percent_Identity=30.5295950155763, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI193205702, Length=355, Percent_Identity=29.0140845070423, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115534089, Length=166, Percent_Identity=36.7469879518072, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923006, Length=416, Percent_Identity=25.9615384615385, Blast_Score=138, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21355641, Length=422, Percent_Identity=27.0142180094787, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24644984, Length=422, Percent_Identity=27.0142180094787, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24668444, Length=121, Percent_Identity=35.5371900826446, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42210; Mature: 42079

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAMAEDDIDDSSDADGEADGLRKIIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
VVAAASYEARRFGVKSAMPSVTAKRLCPDLIFVKHRFEAYREASARIREIFNRYTPHVEP
EEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSLDEAYLDVTDDLQAVGSATGTAQAIRKAIREETGLTASAGVSYNKFLAKLASDQNKPD
CCCCHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
GLCVIRPGEGAAFVQSLPIRRFHGVGPKGAEKMARLGIDTGADLAGKDLAWLRQNFGSFG
CEEEEECCCCHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DYLYRAARGIDLRPIRTNRSRKSVGGERTFSQDQHEPDELRETLERIIAIVWERIERSKA
HHHHHHHCCCCCEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
RGRTITLKMKYNDFRTITRAQSLPHYVSGQDEFASVARDLLEAQLPLPLPIRLMGLTLSN
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LERDEEAQAQRSERKTGQLALF
CCCHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure 
AAAMAEDDIDDSSDADGEADGLRKIIHVDMDAFFASVEQRDNPDLRGKPVAVGGSSGRG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCC
VVAAASYEARRFGVKSAMPSVTAKRLCPDLIFVKHRFEAYREASARIREIFNRYTPHVEP
EEEECCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LSLDEAYLDVTDDLQAVGSATGTAQAIRKAIREETGLTASAGVSYNKFLAKLASDQNKPD
CCCCHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCC
GLCVIRPGEGAAFVQSLPIRRFHGVGPKGAEKMARLGIDTGADLAGKDLAWLRQNFGSFG
CEEEEECCCCHHHHHHCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DYLYRAARGIDLRPIRTNRSRKSVGGERTFSQDQHEPDELRETLERIIAIVWERIERSKA
HHHHHHHCCCCCEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
RGRTITLKMKYNDFRTITRAQSLPHYVSGQDEFASVARDLLEAQLPLPLPIRLMGLTLSN
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LERDEEAQAQRSERKTGQLALF
CCCHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]