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The map label for this gene is hisF [H]

Identifier: 85374738

GI number: 85374738

Start: 1948525

End: 1949382

Strand: Reverse

Name: hisF [H]

Synonym: ELI_09555

Alternate gene names: 85374738

Gene position: 1949382-1948525 (Counterclockwise)

Preceding gene: 85374739

Following gene: 85374737

Centisome position: 63.86

GC content: 67.83

Gene sequence:

>858_bases
GTGACCGTCCGCGTCCGCGTCATTCCCTGTCTCGACGTCGCCGATGGGCGCGTGGTCAAGGGCGTCAATTTCGTCGATCT
CGTCGATGCCGGCGATCCTGTGGAGCAGGCCAAGGCCTATGACGCGGCGGGGGCGGACGAGCTGTGTTTCCTCGATATTT
CCGCGAGCCACGAAGGGCGCGGGACTTTGCTCGATGTGGTGCGGCGCACGGCGGAGCATTGTTTCATGCCGCTGACCGTC
GGCGGAGGGGTGCGCTCGGTCGAGGATGCGCGGGCGCTGCTGCTGGCTGGGGCGGACAAGGTCGCGATCAACAGTGCGGC
GGTGGCGCGGCCCGAACTGGTGCGCGAGATTGCCGACAGGTTCGGCAGCCAGTGTGTCGTCGCGAGTGTGGATGCGCGGC
GCGTGCCTACCGAACCCACCCCCGTTCGGGCTGAGCCTATCGAAGCCCAGCCGCAACGTTCTGCCGCCCCTTCGACAGGC
TCAGGGCGAACGGGGGTGGGGATTTGCCGCTGGGAAATCTACACCCATGGCGGTCGCAAGCCGACCGGCATCGACGCGAT
CGAGTTCGCCGAGCAGGTCGCCGACCTCGGCGCAGGCGAATTGCTCGTCACCAGCATGGATGGCGATGGGACCAAGGCGG
GCTACGACCTGCTGCTGACCCGCACTATCGCCGACAGCGTCTCCGTGCCCGTCATCGCCAGCGGCGGTGTCGGCACGCTC
GATCACCTGGTCGAAGGCGTGACCGAAGGCCATGCCAGCGCGGTGCTGGCGGCCTCCATCTTTCATTTCGGCACGCACAC
CATCGCCGAGGCGCACCAGGCGTTGCGCGATGCAGGCTTGCCCGCGCGCGGCGCTTAG

Upstream 100 bases:

>100_bases
CGCGTGGCCTTCGACAGGCTCAGGCCGAACGGAACCTGTGCTTGGCCCCAACCCCGTTCATCCTGAGCCTGTCGAAGGAC
AGGCGAAAGGGCGGAAAACC

Downstream 100 bases:

>100_bases
GCGAGATGGAGAGTATGAATACGCTGCAACGCCTCGAGGCCACCATCGCCGCGCGCCGCAATGCCGATCCCGACAGCAGT
TATGTCGCGCGGCTCAATGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLVDAGDPVEQAKAYDAAGADELCFLDISASHEGRGTLLDVVRRTAEHCFMPLTV
GGGVRSVEDARALLLAGADKVAINSAAVARPELVREIADRFGSQCVVASVDARRVPTEPTPVRAEPIEAQPQRSAAPSTG
SGRTGVGICRWEIYTHGGRKPTGIDAIEFAEQVADLGAGELLVTSMDGDGTKAGYDLLLTRTIADSVSVPVIASGGVGTL
DHLVEGVTEGHASAVLAASIFHFGTHTIAEAHQALRDAGLPARGA

Sequences:

>Translated_285_residues
MTVRVRVIPCLDVADGRVVKGVNFVDLVDAGDPVEQAKAYDAAGADELCFLDISASHEGRGTLLDVVRRTAEHCFMPLTV
GGGVRSVEDARALLLAGADKVAINSAAVARPELVREIADRFGSQCVVASVDARRVPTEPTPVRAEPIEAQPQRSAAPSTG
SGRTGVGICRWEIYTHGGRKPTGIDAIEFAEQVADLGAGELLVTSMDGDGTKAGYDLLLTRTIADSVSVPVIASGGVGTL
DHLVEGVTEGHASAVLAASIFHFGTHTIAEAHQALRDAGLPARGA
>Mature_284_residues
TVRVRVIPCLDVADGRVVKGVNFVDLVDAGDPVEQAKAYDAAGADELCFLDISASHEGRGTLLDVVRRTAEHCFMPLTVG
GGVRSVEDARALLLAGADKVAINSAAVARPELVREIADRFGSQCVVASVDARRVPTEPTPVRAEPIEAQPQRSAAPSTGS
GRTGVGICRWEIYTHGGRKPTGIDAIEFAEQVADLGAGELLVTSMDGDGTKAGYDLLLTRTIADSVSVPVIASGGVGTLD
HLVEGVTEGHASAVLAASIFHFGTHTIAEAHQALRDAGLPARGA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=278, Percent_Identity=37.0503597122302, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI87082028, Length=276, Percent_Identity=26.4492753623188, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.4465408805031, Blast_Score=146, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 29564; Mature: 29432

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRVRVIPCLDVADGRVVKGVNFVDLVDAGDPVEQAKAYDAAGADELCFLDISASHEGR
CEEEEEEEEEEECCCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GTLLDVVRRTAEHCFMPLTVGGGVRSVEDARALLLAGADKVAINSAAVARPELVREIADR
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHEEEEECCCCEEECCHHHCCHHHHHHHHHH
FGSQCVVASVDARRVPTEPTPVRAEPIEAQPQRSAAPSTGSGRTGVGICRWEIYTHGGRK
HCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
PTGIDAIEFAEQVADLGAGELLVTSMDGDGTKAGYDLLLTRTIADSVSVPVIASGGVGTL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHEEEHHHHCCCCCCEEECCCCCHH
DHLVEGVTEGHASAVLAASIFHFGTHTIAEAHQALRDAGLPARGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVRVRVIPCLDVADGRVVKGVNFVDLVDAGDPVEQAKAYDAAGADELCFLDISASHEGR
EEEEEEEEEEECCCCCEECCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEECCCCCCC
GTLLDVVRRTAEHCFMPLTVGGGVRSVEDARALLLAGADKVAINSAAVARPELVREIADR
HHHHHHHHHHHHHCEEEEEECCCCCCHHHHHEEEEECCCCEEECCHHHCCHHHHHHHHHH
FGSQCVVASVDARRVPTEPTPVRAEPIEAQPQRSAAPSTGSGRTGVGICRWEIYTHGGRK
HCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCC
PTGIDAIEFAEQVADLGAGELLVTSMDGDGTKAGYDLLLTRTIADSVSVPVIASGGVGTL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHEEEHHHHCCCCCCEEECCCCCHH
DHLVEGVTEGHASAVLAASIFHFGTHTIAEAHQALRDAGLPARGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA