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The map label for this gene is hisA

Identifier: 85374739

GI number: 85374739

Start: 1949489

End: 1950217

Strand: Reverse

Name: hisA

Synonym: ELI_09560

Alternate gene names: 85374739

Gene position: 1950217-1949489 (Counterclockwise)

Preceding gene: 85374740

Following gene: 85374738

Centisome position: 63.89

GC content: 66.26

Gene sequence:

>729_bases
ATGATCGTCTTTCCCGCCATCGACCTCAAGGGCGGCGAAGTCGTGCGGCTGGCCGAGGGCGATATGGATCGCGCCACCGT
CTATGGCGACAACCCGGCAGCGCAGGCGATGCTGTTTGCCGAGGCCGGGGCCGAGCACCTGCACGTAGTCGATCTCGACG
GTGCTTTCGCGGGCGAGAGCCGCAATCGCGAGGCTGTCGAAGCGATCGTTGCGGAGTTCCCCGGCTATGTGCAGCTCGGC
GGCGGCATTCGCGATGCCACGGCGGTCGAAGGCTGGTTTAACCTCGGCGTCGCGCGAATCGTGATTGGGTCGGCTGCGCT
CAAGGACCCGGATTTCGTCAAAGAGATGGCGCGCGAGTGGGAGAATGGCATCGTCGTCGCTGTGGATGCGCGCGACGGCA
TGATTGCGACCGAGGGCTGGGCCGAAGTCTCCGACGTCCCCGTCGCCGACATGGCGCGCCGCTTCGAGGATGCGGGCGTC
GCCAGCCTGCTGTTCACCGACATCGGCCGCGACGGCCTGCTCAAGGGCTGCAATATCGACGCGACGGTCGAGCTCGCCCG
CCAGACCGACATGCCCGTGATTGCCAGCGGCGGCGTCAAGGGACTGGACGATATCCACGTCCTCTCGCTGCATGCGCATG
AGGGGATCGAAGGGGTCATTACCGGGCGCGCCCTTTACGAAGGGCGGCTGGACTTGGCCGCGGCGATTGCAATGGGGGCG
AGGAGCTAA

Upstream 100 bases:

>100_bases
CGGTGGCGCGGGATAATATCGTCGGCGTGCAATTTCACCCGGAGAAGAGCCAAGCCTATGGGCTGGGGCTGCTCACCCGT
TTTCTCGATTGGAACCCGGC

Downstream 100 bases:

>100_bases
TGTCGGCGCGTGGCCTTCGACAGGCTCAGGCCGAACGGAACCTGTGCTTGGCCCCAACCCCGTTCATCCTGAGCCTGTCG
AAGGACAGGCGAAAGGGCGG

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGESRNREAVEAIVAEFPGYVQLG
GGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREWENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGV
ASLLFTDIGRDGLLKGCNIDATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA
RS

Sequences:

>Translated_242_residues
MIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGESRNREAVEAIVAEFPGYVQLG
GGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREWENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGV
ASLLFTDIGRDGLLKGCNIDATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA
RS
>Mature_242_residues
MIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGESRNREAVEAIVAEFPGYVQLG
GGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREWENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGV
ASLLFTDIGRDGLLKGCNIDATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA
RS

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=37.2950819672131, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_ERYLH (Q2N8I7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458801.1
- HSSP:   P16250
- ProteinModelPortal:   Q2N8I7
- SMR:   Q2N8I7
- STRING:   Q2N8I7
- GeneID:   3869495
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_09560
- NMPDR:   fig|314225.3.peg.1127
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- PhylomeDB:   Q2N8I7
- ProtClustDB:   PRK00748
- BioCyc:   ELIT314225:ELI_09560-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25472; Mature: 25472

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGES
CEEEEEEECCCCCEEEEECCCCCCEEEECCCHHHHEEEEECCCCCEEEEEECCCCCCCCC
RNREAVEAIVAEFPGYVQLGGGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREW
CCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCHHHHHCCHHCCCCHHHHHHHHHHH
ENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGVASLLFTDIGRDGLLKGCNID
HCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEECCCCC
ATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA
CEEEEHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHCC
RS
CC
>Mature Secondary Structure
MIVFPAIDLKGGEVVRLAEGDMDRATVYGDNPAAQAMLFAEAGAEHLHVVDLDGAFAGES
CEEEEEEECCCCCEEEEECCCCCCEEEECCCHHHHEEEEECCCCCEEEEEECCCCCCCCC
RNREAVEAIVAEFPGYVQLGGGIRDATAVEGWFNLGVARIVIGSAALKDPDFVKEMAREW
CCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCHHHHHCCHHCCCCHHHHHHHHHHH
ENGIVVAVDARDGMIATEGWAEVSDVPVADMARRFEDAGVASLLFTDIGRDGLLKGCNID
HCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEECCCCC
ATVELARQTDMPVIASGGVKGLDDIHVLSLHAHEGIEGVITGRALYEGRLDLAAAIAMGA
CEEEEHHHCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHCC
RS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA