Click here to switch to the map view.

The map label for this gene is blaNDM-1 [H]

Identifier: 85374343

GI number: 85374343

Start: 1569015

End: 1569800

Strand: Direct

Name: blaNDM-1 [H]

Synonym: ELI_07580

Alternate gene names: 85374343

Gene position: 1569015-1569800 (Clockwise)

Preceding gene: 85374342

Following gene: 85374344

Centisome position: 51.4

GC content: 65.78

Gene sequence:

>786_bases
GTGATCGCGCGGGTGCTGGCGCTCGCCGCCGCGCTGGCGCTGCCCGCCTGCGTGCCCGGCGAAATCCGCAGTTCGGTCGC
CGAGCAGGAGGCCGACCGCGACATCATCCGCTTCGGCGAAGTGTCGTTCAGCCAATTGGCCGAGGGCGTGTGGATGCACA
CCACCTATCTCGACCTGATGGGCTTCGGGCCGATCCCTTCGAATGGTCTGTTGGTGGTAAACGGCGATAACACAATCCTC
GTCGATACCGCCTGGACCGACGAACAGACCGAGCAAATCGTCGCCTGGGCCAGCATGGTCCTGGCCAAGCCCGTCCGCGC
TGCGGTCGTCACCCATGCGCATCAGGACAAGATGGGCGGCATGGCCGCGCTGCACGGCGCGAACATCGCGACCTGGGCGC
ATCCGCTGAGCAACGAACTGGCGCCCGAGGAAGGGCTGGTTCCTGCACGCAATGCCATCACCTTCGACGCAAACGGTTGG
GCGACGGGCGAGGCGGCGCAATCGCTTGCGCCCTTACGCCTCTATTACCCCGGCGGAGCGCATACGCGCGACAACATCAC
CGTCGGCCTGCCGGAGCTTGGAATCGCCTTCGGAGGCTGCATGATCAAGGCGGGGGACGCCTCGAACCTCGGCAATCTCG
CCGATGCGGACACCGCGGCCTATGCGCAATCGGTGCGTAATTTCGCAGCGGCGTTCCCCGATGCCCGGACCATTGCGATG
AGCCATTCCCCGCCCGAGGGTCGCAAGGCGATCGAGCGCACGCTTGATCTCGCCGAAGAACTCTAG

Upstream 100 bases:

>100_bases
GCCAGGCGCGTGACGACGGTGCCAGCGTCCTGGTGATCGGCCGCCCGATCAGCAAGGCGGACGATCCGGAACAGGCTGCG
CGGGATATCGAGGCGACGCT

Downstream 100 bases:

>100_bases
CAACGCGCGCGCCATGACGATGATCAAGATTTGCGGACTCTCCACGCCGGAGACGATCGAGGCTGCCGTGCAGGCGGGCG
CGACCCATGTCGGCTTGGTG

Product: beta-lactamase II

Products: NA

Alternate protein names: NDM-1; Metallo-beta-lactamase NDM-1 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIARVLALAAALALPACVPGEIRSSVAEQEADRDIIRFGEVSFSQLAEGVWMHTTYLDLMGFGPIPSNGLLVVNGDNTIL
VDTAWTDEQTEQIVAWASMVLAKPVRAAVVTHAHQDKMGGMAALHGANIATWAHPLSNELAPEEGLVPARNAITFDANGW
ATGEAAQSLAPLRLYYPGGAHTRDNITVGLPELGIAFGGCMIKAGDASNLGNLADADTAAYAQSVRNFAAAFPDARTIAM
SHSPPEGRKAIERTLDLAEEL

Sequences:

>Translated_261_residues
MIARVLALAAALALPACVPGEIRSSVAEQEADRDIIRFGEVSFSQLAEGVWMHTTYLDLMGFGPIPSNGLLVVNGDNTIL
VDTAWTDEQTEQIVAWASMVLAKPVRAAVVTHAHQDKMGGMAALHGANIATWAHPLSNELAPEEGLVPARNAITFDANGW
ATGEAAQSLAPLRLYYPGGAHTRDNITVGLPELGIAFGGCMIKAGDASNLGNLADADTAAYAQSVRNFAAAFPDARTIAM
SHSPPEGRKAIERTLDLAEEL
>Mature_261_residues
MIARVLALAAALALPACVPGEIRSSVAEQEADRDIIRFGEVSFSQLAEGVWMHTTYLDLMGFGPIPSNGLLVVNGDNTIL
VDTAWTDEQTEQIVAWASMVLAKPVRAAVVTHAHQDKMGGMAALHGANIATWAHPLSNELAPEEGLVPARNAITFDANGW
ATGEAAQSLAPLRLYYPGGAHTRDNITVGLPELGIAFGGCMIKAGDASNLGNLADADTAAYAQSVRNFAAAFPDARTIAM
SHSPPEGRKAIERTLDLAEEL

Specific function: Confers resistance to many beta-lactam antibiotics, including some carbapenems. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential). Note=The predicted signal sequence is not very convincing [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 27509; Mature: 27509

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00014 ER_TARGET

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARVLALAAALALPACVPGEIRSSVAEQEADRDIIRFGEVSFSQLAEGVWMHTTYLDLM
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCHHHHHHHHHHH
GFGPIPSNGLLVVNGDNTILVDTAWTDEQTEQIVAWASMVLAKPVRAAVVTHAHQDKMGG
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MAALHGANIATWAHPLSNELAPEEGLVPARNAITFDANGWATGEAAQSLAPLRLYYPGGA
EEEECCCCHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCCC
HTRDNITVGLPELGIAFGGCMIKAGDASNLGNLADADTAAYAQSVRNFAAAFPDARTIAM
CCCCCEEECCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
SHSPPEGRKAIERTLDLAEEL
CCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIARVLALAAALALPACVPGEIRSSVAEQEADRDIIRFGEVSFSQLAEGVWMHTTYLDLM
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCHHHHHHHHHHH
GFGPIPSNGLLVVNGDNTILVDTAWTDEQTEQIVAWASMVLAKPVRAAVVTHAHQDKMGG
CCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
MAALHGANIATWAHPLSNELAPEEGLVPARNAITFDANGWATGEAAQSLAPLRLYYPGGA
EEEECCCCHHHHCCCHHCCCCCCCCCCCCCCEEEECCCCCCCCHHHHCCCCEEEEECCCC
HTRDNITVGLPELGIAFGGCMIKAGDASNLGNLADADTAAYAQSVRNFAAAFPDARTIAM
CCCCCEEECCCHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
SHSPPEGRKAIERTLDLAEEL
CCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA