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The map label for this gene is trpF

Identifier: 85374344

GI number: 85374344

Start: 1569814

End: 1570443

Strand: Direct

Name: trpF

Synonym: ELI_07585

Alternate gene names: 85374344

Gene position: 1569814-1570443 (Clockwise)

Preceding gene: 85374343

Following gene: 162007984

Centisome position: 51.43

GC content: 63.33

Gene sequence:

>630_bases
ATGACGATGATCAAGATTTGCGGACTCTCCACGCCGGAGACGATCGAGGCTGCCGTGCAGGCGGGCGCGACCCATGTCGG
CTTGGTGCATTTCGCCAAGAGCCCGCGGCATGTGGAACTAGAGAAAGCCGCCGAATTGCGCGCGCTGGTGCCCGAGAGCG
TCAAGGCGGTGCTGTTGCTGGTCAACGAGCAGCCCGAAGAGACCGCGCGCGCGATCCAGATCGTCAAGCCCGACGTCGTG
CAGTTTCATGGCAGCGAAACGCCGCAATGGACCAAGGCGGTGCGCGACCAGCTTGGTATCGAGGTGTGGAAAGCGCTGGG
CGTACGCGAAGCGGCTACGCTGGAGAAATCGCGCCGCTATGAAGGCGCGGTCGACCGGCTGCTGTTCGATTCCCCGGCAA
AGAAGTTGCCGGGCGGCAATGGAGTGACGTTCCAGTGGGACGTGCTCGCCGGCTTCGAGCACCATACGGCCTGGGGGCTG
GCAGGCGGGCTGACGCCGGACAACGTCGGTGATGCGATCCGCCAAACCGGCGCGGAGCTTGTCGATGCCTCAAGTGGTGT
TGAGAGCGCTCCGGGCGTCAAGGATATTGCGAAAATCGAAGCGTTCTGCGAAGCCGCAAGAAACGCATAG

Upstream 100 bases:

>100_bases
GATGCCCGGACCATTGCGATGAGCCATTCCCCGCCCGAGGGTCGCAAGGCGATCGAGCGCACGCTTGATCTCGCCGAAGA
ACTCTAGCAACGCGCGCGCC

Downstream 100 bases:

>100_bases
ACCCCCTACTCCCGTTCGCCCTGTGCTTGTCGAAGGGCCGTACTTCTTTTTTACGCTGCGCCAGAAGCAAGAACGGGGCT
TCGACAGGCTCAGCCCGAAC

Product: N-(5'phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTMIKICGLSTPETIEAAVQAGATHVGLVHFAKSPRHVELEKAAELRALVPESVKAVLLLVNEQPEETARAIQIVKPDVV
QFHGSETPQWTKAVRDQLGIEVWKALGVREAATLEKSRRYEGAVDRLLFDSPAKKLPGGNGVTFQWDVLAGFEHHTAWGL
AGGLTPDNVGDAIRQTGAELVDASSGVESAPGVKDIAKIEAFCEAARNA

Sequences:

>Translated_209_residues
MTMIKICGLSTPETIEAAVQAGATHVGLVHFAKSPRHVELEKAAELRALVPESVKAVLLLVNEQPEETARAIQIVKPDVV
QFHGSETPQWTKAVRDQLGIEVWKALGVREAATLEKSRRYEGAVDRLLFDSPAKKLPGGNGVTFQWDVLAGFEHHTAWGL
AGGLTPDNVGDAIRQTGAELVDASSGVESAPGVKDIAKIEAFCEAARNA
>Mature_208_residues
TMIKICGLSTPETIEAAVQAGATHVGLVHFAKSPRHVELEKAAELRALVPESVKAVLLLVNEQPEETARAIQIVKPDVVQ
FHGSETPQWTKAVRDQLGIEVWKALGVREAATLEKSRRYEGAVDRLLFDSPAKKLPGGNGVTFQWDVLAGFEHHTAWGLA
GGLTPDNVGDAIRQTGAELVDASSGVESAPGVKDIAKIEAFCEAARNA

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=193, Percent_Identity=35.7512953367876, Blast_Score=93, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320210, Length=219, Percent_Identity=31.0502283105023, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_ERYLH (Q2N9N2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458406.1
- ProteinModelPortal:   Q2N9N2
- SMR:   Q2N9N2
- STRING:   Q2N9N2
- GeneID:   3871535
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07585
- NMPDR:   fig|314225.3.peg.1525
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- PhylomeDB:   Q2N9N2
- ProtClustDB:   CLSK753564
- BioCyc:   ELIT314225:ELI_07585-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 22365; Mature: 22233

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMIKICGLSTPETIEAAVQAGATHVGLVHFAKSPRHVELEKAAELRALVPESVKAVLLL
CCEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCCEEEHHHHHHHHHHCHHHHHEEEEE
VNEQPEETARAIQIVKPDVVQFHGSETPQWTKAVRDQLGIEVWKALGVREAATLEKSRRY
ECCCCHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EGAVDRLLFDSPAKKLPGGNGVTFQWDVLAGFEHHTAWGLAGGLTPDNVGDAIRQTGAEL
HHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDASSGVESAPGVKDIAKIEAFCEAARNA
HHCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TMIKICGLSTPETIEAAVQAGATHVGLVHFAKSPRHVELEKAAELRALVPESVKAVLLL
CEEEEECCCCCHHHHHHHHHCCCHHHHHHCCCCCCEEEHHHHHHHHHHCHHHHHEEEEE
VNEQPEETARAIQIVKPDVVQFHGSETPQWTKAVRDQLGIEVWKALGVREAATLEKSRRY
ECCCCHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EGAVDRLLFDSPAKKLPGGNGVTFQWDVLAGFEHHTAWGLAGGLTPDNVGDAIRQTGAEL
HHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDASSGVESAPGVKDIAKIEAFCEAARNA
HHCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA