Click here to switch to the map view.

The map label for this gene is pyrF

Identifier: 85374342

GI number: 85374342

Start: 1568344

End: 1569018

Strand: Direct

Name: pyrF

Synonym: ELI_07575

Alternate gene names: 85374342

Gene position: 1568344-1569018 (Clockwise)

Preceding gene: 85374341

Following gene: 85374343

Centisome position: 51.38

GC content: 65.93

Gene sequence:

>675_bases
GTGAGCAACCCGGTCTTCCTCGCGCTCGACATCCCGCGGCTGGAAGCGGGCAAGGCGCTGGTCGACAAGGTCAAGGCGCA
TATCGGTGGTGTGAAGCTCGGCATGGAGTTCTTCTATGCCCACGGCCATCACGGGGTGCACGAAATTGCGCACTGTGGTC
TGCCGGTCTTCCTCGACCTCAAGCTGCACGACATTCCCAACACCGTCGCCGCCGCGATGCAATCGATCCATGTGCTGGAG
CCGGCCATCGTCACGGTCCACGCATCGGGCGGGCGCGCGATGATGGAAGACGCCAAGGCGGCGGCGGGCGAGAACACCAA
GGTCGTCGGCGTAACCATGCTGACCAGCCTGGACGAGCGCGACCTCGAACGCACCGGGGTCGATGGCAGCCCGCACGATC
ATGTCATGCGGCTGGCCGAACTGGCGGAGAACGCCGGGCTCGACGGGATCGTCTGCTCCGGACAGGAAGTCGGCGCGGTA
CACAAGCAATGGAAACAGGGCTTCTTCGTTGTTCCCGGCCTGCGCCCGGCAGGCAGCGCAAGTGGTGACCAGAAGCGCGT
CGTCACCCCACGCCAGGCGCGTGACGACGGTGCCAGCGTCCTGGTGATCGGCCGCCCGATCAGCAAGGCGGACGATCCGG
AACAGGCTGCGCGGGATATCGAGGCGACGCTGTGA

Upstream 100 bases:

>100_bases
CGTCGACGGAAGATCCAGCGCCCGCACCTACGCCTGCATCGAGCACACCGCCCCCGCCGCAGCCCGACAGCGACGGCCTC
CAGCCGGAAACCGACAGACC

Downstream 100 bases:

>100_bases
TCGCGCGGGTGCTGGCGCTCGCCGCCGCGCTGGCGCTGCCCGCCTGCGTGCCCGGCGAAATCCGCAGTTCGGTCGCCGAG
CAGGAGGCCGACCGCGACAT

Product: orotidine-5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSNPVFLALDIPRLEAGKALVDKVKAHIGGVKLGMEFFYAHGHHGVHEIAHCGLPVFLDLKLHDIPNTVAAAMQSIHVLE
PAIVTVHASGGRAMMEDAKAAAGENTKVVGVTMLTSLDERDLERTGVDGSPHDHVMRLAELAENAGLDGIVCSGQEVGAV
HKQWKQGFFVVPGLRPAGSASGDQKRVVTPRQARDDGASVLVIGRPISKADDPEQAARDIEATL

Sequences:

>Translated_224_residues
MSNPVFLALDIPRLEAGKALVDKVKAHIGGVKLGMEFFYAHGHHGVHEIAHCGLPVFLDLKLHDIPNTVAAAMQSIHVLE
PAIVTVHASGGRAMMEDAKAAAGENTKVVGVTMLTSLDERDLERTGVDGSPHDHVMRLAELAENAGLDGIVCSGQEVGAV
HKQWKQGFFVVPGLRPAGSASGDQKRVVTPRQARDDGASVLVIGRPISKADDPEQAARDIEATL
>Mature_223_residues
SNPVFLALDIPRLEAGKALVDKVKAHIGGVKLGMEFFYAHGHHGVHEIAHCGLPVFLDLKLHDIPNTVAAAMQSIHVLEP
AIVTVHASGGRAMMEDAKAAAGENTKVVGVTMLTSLDERDLERTGVDGSPHDHVMRLAELAENAGLDGIVCSGQEVGAVH
KQWKQGFFVVPGLRPAGSASGDQKRVVTPRQARDDGASVLVIGRPISKADDPEQAARDIEATL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=42.1052631578947, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_ERYLH (Q2N9N4)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458404.1
- ProteinModelPortal:   Q2N9N4
- STRING:   Q2N9N4
- GeneID:   3871533
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_07575
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2N9N4
- ProtClustDB:   CLSK835196
- BioCyc:   ELIT314225:ELI_07575-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 23733; Mature: 23602

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 115-115 BINDING 175-175 BINDING 184-184 BINDING 204-204 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNPVFLALDIPRLEAGKALVDKVKAHIGGVKLGMEFFYAHGHHGVHEIAHCGLPVFLDL
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHEECCCCCHHHHHHCCCCEEEEE
KLHDIPNTVAAAMQSIHVLEPAIVTVHASGGRAMMEDAKAAAGENTKVVGVTMLTSLDER
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEHHCCHH
DLERTGVDGSPHDHVMRLAELAENAGLDGIVCSGQEVGAVHKQWKQGFFVVPGLRPAGSA
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCEEEECCCCCCCCC
SGDQKRVVTPRQARDDGASVLVIGRPISKADDPEQAARDIEATL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SNPVFLALDIPRLEAGKALVDKVKAHIGGVKLGMEFFYAHGHHGVHEIAHCGLPVFLDL
CCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHEECCCCCHHHHHHCCCCEEEEE
KLHDIPNTVAAAMQSIHVLEPAIVTVHASGGRAMMEDAKAAAGENTKVVGVTMLTSLDER
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEHHCCHH
DLERTGVDGSPHDHVMRLAELAENAGLDGIVCSGQEVGAVHKQWKQGFFVVPGLRPAGSA
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCEEEECCCCCCCCC
SGDQKRVVTPRQARDDGASVLVIGRPISKADDPEQAARDIEATL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA