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The map label for this gene is holB [H]

Identifier: 85374071

GI number: 85374071

Start: 1293449

End: 1294411

Strand: Direct

Name: holB [H]

Synonym: ELI_06220

Alternate gene names: 85374071

Gene position: 1293449-1294411 (Clockwise)

Preceding gene: 85374070

Following gene: 85374072

Centisome position: 42.37

GC content: 64.38

Gene sequence:

>963_bases
ATGCGGCTCACGCATGCCGAAATCCAGCGCGAGTGGGATGCGGCACTGGCTGGCCCGCGAATGCATCACGGATGGATTCT
TGCCGGGCGGCAGGGGCTTGGCAAATCGGAATTTGCGCTCGACGCCGCGCGGCAGCTTGTTGCGCGGGGAGCTACCGCGC
CACAGCCCGATGGCGAACATCCCGATATCCACCTGCTTTCGCGTCCGCCGAAGGACGACAAGGAAGTTCGCAAGCGCGCC
GAAGGCAAGCCGTTCGAGCGCAAACGCAACATCTCGGTCGACCAAATCCGTGCGATGCAGCGGCGCTTGACGACCCGCCC
GACGCTGGGAAGAAATCGCGCGATCATTATCGATCCCGCGGATGATCTGGAAAAGGGCGCGTCCAATGCGCTGCTCAAGA
GCCTCGAGGAACCACCGGTAGGTACCTTCTTCATCCTCGTGACCCATCGCCTGGCGCGTTTGTTGCCCACAATCCGGTCG
CGGTGCCGGGTGCTGCGGTTCAATCCCTTGCCCGATGCGCAGGTCGAGAAGCTCCTAAAGGAAGAATCGCCGTCGAGCGA
TGCTGCGGTGCGCGAGGCGGCCGTTGCGGCGGCAAAGGGGTCACCGGGCGCGGCGATCAAGTTTATAGAGCAGGACCTCG
CGCCGCTGGCGGCGATCATGCGGGACATCGTAGCGCAAGGCGATCGGGCGTTCGAACTGCGCGGCGCGCTTTCGGCTGCG
ATCGGAGCGCGCCCCAACCGGGAGCGGGTCCAGGCGACATTCGACCTCGCACGGACGATTCTGGCCGAGCAGATGGCCAC
GGTTCCGAGCGCACAATTGATGCAACTGGCCGATGCCCATGCCGACATGGTGGCCCTCGCCGGCCAGGCCCCGACCTACA
ATTTCGATCCCGGATTTCTCGCGATGGAAATCGGCAGCTTGCTAGCCTCGGCTGCGCCCCATACAGAGCGCGCCAATGTC
TGA

Upstream 100 bases:

>100_bases
ACCCGGGAGGCTTTGCCGTAGTCGATGGTGAGGGCACGCCCGATGAGGTCCACTCGCGGATTCTAGAAGCAATTGAGCCC
CTTATCGGAGGCGAACTCTG

Downstream 100 bases:

>100_bases
GCCATTCTATATTACCACCGCGATCAGCTACCCCAACGGGCGGCCGCACATCGGCCATGCTTACGAAGCGATCGCTGCCG
ATGTCATTGCGCGATTCCAG

Product: DNA polymerase III delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRLTHAEIQREWDAALAGPRMHHGWILAGRQGLGKSEFALDAARQLVARGATAPQPDGEHPDIHLLSRPPKDDKEVRKRA
EGKPFERKRNISVDQIRAMQRRLTTRPTLGRNRAIIIDPADDLEKGASNALLKSLEEPPVGTFFILVTHRLARLLPTIRS
RCRVLRFNPLPDAQVEKLLKEESPSSDAAVREAAVAAAKGSPGAAIKFIEQDLAPLAAIMRDIVAQGDRAFELRGALSAA
IGARPNRERVQATFDLARTILAEQMATVPSAQLMQLADAHADMVALAGQAPTYNFDPGFLAMEIGSLLASAAPHTERANV

Sequences:

>Translated_320_residues
MRLTHAEIQREWDAALAGPRMHHGWILAGRQGLGKSEFALDAARQLVARGATAPQPDGEHPDIHLLSRPPKDDKEVRKRA
EGKPFERKRNISVDQIRAMQRRLTTRPTLGRNRAIIIDPADDLEKGASNALLKSLEEPPVGTFFILVTHRLARLLPTIRS
RCRVLRFNPLPDAQVEKLLKEESPSSDAAVREAAVAAAKGSPGAAIKFIEQDLAPLAAIMRDIVAQGDRAFELRGALSAA
IGARPNRERVQATFDLARTILAEQMATVPSAQLMQLADAHADMVALAGQAPTYNFDPGFLAMEIGSLLASAAPHTERANV
>Mature_320_residues
MRLTHAEIQREWDAALAGPRMHHGWILAGRQGLGKSEFALDAARQLVARGATAPQPDGEHPDIHLLSRPPKDDKEVRKRA
EGKPFERKRNISVDQIRAMQRRLTTRPTLGRNRAIIIDPADDLEKGASNALLKSLEEPPVGTFFILVTHRLARLLPTIRS
RCRVLRFNPLPDAQVEKLLKEESPSSDAAVREAAVAAAKGSPGAAIKFIEQDLAPLAAIMRDIVAQGDRAFELRGALSAA
IGARPNRERVQATFDLARTILAEQMATVPSAQLMQLADAHADMVALAGQAPTYNFDPGFLAMEIGSLLASAAPHTERANV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=206, Percent_Identity=32.0388349514563, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786676, Length=204, Percent_Identity=26.4705882352941, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34853; Mature: 34853

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTHAEIQREWDAALAGPRMHHGWILAGRQGLGKSEFALDAARQLVARGATAPQPDGEH
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDIHLLSRPPKDDKEVRKRAEGKPFERKRNISVDQIRAMQRRLTTRPTLGRNRAIIIDPA
CCEEECCCCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCH
DDLEKGASNALLKSLEEPPVGTFFILVTHRLARLLPTIRSRCRVLRFNPLPDAQVEKLLK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHH
EESPSSDAAVREAAVAAAKGSPGAAIKFIEQDLAPLAAIMRDIVAQGDRAFELRGALSAA
HCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IGARPNRERVQATFDLARTILAEQMATVPSAQLMQLADAHADMVALAGQAPTYNFDPGFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AMEIGSLLASAAPHTERANV
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLTHAEIQREWDAALAGPRMHHGWILAGRQGLGKSEFALDAARQLVARGATAPQPDGEH
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDIHLLSRPPKDDKEVRKRAEGKPFERKRNISVDQIRAMQRRLTTRPTLGRNRAIIIDPA
CCEEECCCCCCCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCH
DDLEKGASNALLKSLEEPPVGTFFILVTHRLARLLPTIRSRCRVLRFNPLPDAQVEKLLK
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHH
EESPSSDAAVREAAVAAAKGSPGAAIKFIEQDLAPLAAIMRDIVAQGDRAFELRGALSAA
HCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IGARPNRERVQATFDLARTILAEQMATVPSAQLMQLADAHADMVALAGQAPTYNFDPGFL
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
AMEIGSLLASAAPHTERANV
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]