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The map label for this gene is metG [H]

Identifier: 85374072

GI number: 85374072

Start: 1294404

End: 1295963

Strand: Direct

Name: metG [H]

Synonym: ELI_06225

Alternate gene names: 85374072

Gene position: 1294404-1295963 (Clockwise)

Preceding gene: 85374071

Following gene: 85374073

Centisome position: 42.41

GC content: 61.47

Gene sequence:

>1560_bases
ATGTCTGAGCCATTCTATATTACCACCGCGATCAGCTACCCCAACGGGCGGCCGCACATCGGCCATGCTTACGAAGCGAT
CGCTGCCGATGTCATTGCGCGATTCCAGCGCCAGAGGGGCCTCGACGTACGGTTCCAGACCGGGACCGACGAGCACGGCC
TCAAAATGGCGCGCAAGGCGGAGGAGCAGGGTAAGTCGCCGCGCGACCTGGCGGACGAAATGTCGGGATATTTCAAAGCC
ATGTGCGACGCTTTGAATGTTTCCTGCGACCGCTTCATCCGCACCGTCGATGACGACCACCACCGCGCGAGCCAGGCGAT
CTGGCGGGCAATGGAGGCTGCGGGCGACCTCTATCTCGACCGTTACGAAGGCTGGTACTCGGTCCGCGACGAAGCCTATT
ATGACGAGAGCGAGCTGGTAGACGGCGAGGGCGACGAGAAGCTCTCGCCGCAGGGTACGCCGGTCGAATGGACGGTCGAG
GAAAGCTGGTTCTTCCGCCTGTCGAAATACGAAGACAAGCTGCTCAAGCTGCTGGAAAGCCCGGGCTTTCTCGAGCCCGC
CAGTCGCCGCAACGAAATGGTCGCCTTCGTCAAGCAAGGCCTGCGCGATCTCTCGGTCAGCCGAACCAGCTTCGACTGGG
GCGTAAAGGTCCCCGGCAGCGAAGATCATGTGATGTACGTCTGGGTCGATGCCCTGACCAATTACATCACCGGCCTCGGC
TATCCCGATGATACCGAGCTGTGGCGCAAGCATTGGCCCGCCGATCTGCACCTGATCGGCAAGGATATCGTTCGCTTCCA
CACCATCTACTGGCCGGCGTTTCTGATGAGCGCCGGGCTGCCGGTGCCGAAGCAGGTGTTCGGGCACGGTTTCGTGCTGA
AGGACGGGCGCAAGGAATCGAAATCGCTCGGCAACGTCACCGATCCGCATGACCTCGCCGATTTCTACGGCGTCGATGCG
CTGCGCTATTTCCTGATGCGCGAGGTCTCGTTCGGATCGGACGGCAACTGGTCGCACGAAGCGATCGTGACCCGCGTCAA
TGCCGAGCTTGCCAACAGTTTCGGAAACCTTGCGCAGCGGACATTGTCGATGATCCACAAGAACATGGACGGCTCGCTGG
AGAAATTCGAAGCGCGCGGTGAGGACAAGGCGCTGCTGCAGACTGTGCTCACCGCCTGCCGCGAAAGCTTGCCGCGCGAG
TTCGAGAAGCTCGCTTTTTCGGTCGGCATCGATGAGTGGATCAAGGCGGTCTATGCCTGCAACCAGTATGTCGACGAACA
GGCGCCGTGGGCGCTCAGGAAGACCGATCCCGAGCGGATGAAGGCGGTGCTCCAGACCTTGCTGATGGCAATCCACGATC
TCGCCATCGCGATCCAGCCCGTCATTCCGGAGAAGGCTGGTGCCTTGCTAGACGACCTCGGCGTGCCAACCGACCGCCGG
ACGTACGATCACATTTCCGGGGAAGGGTGGTTCAACCAGCTCGTCGCCGAAGGCTTTACCGTGCCCAAGCCGACCCCCGC
CTTCCCGCGCCTCGAAATGCCAGAGAGCGAGGATGCCTGA

Upstream 100 bases:

>100_bases
CTCGCCGGCCAGGCCCCGACCTACAATTTCGATCCCGGATTTCTCGCGATGGAAATCGGCAGCTTGCTAGCCTCGGCTGC
GCCCCATACAGAGCGCGCCA

Downstream 100 bases:

>100_bases
TGCTGGTCGATAGCCATTGCCACCTGCAGTACAAAGGCCTGGTCGAAGATCAGCAGGCCGTGCTCGAACGCGCGCGGGCA
GCAGGCGTGCAGGGCATGCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSEPFYITTAISYPNGRPHIGHAYEAIAADVIARFQRQRGLDVRFQTGTDEHGLKMARKAEEQGKSPRDLADEMSGYFKA
MCDALNVSCDRFIRTVDDDHHRASQAIWRAMEAAGDLYLDRYEGWYSVRDEAYYDESELVDGEGDEKLSPQGTPVEWTVE
ESWFFRLSKYEDKLLKLLESPGFLEPASRRNEMVAFVKQGLRDLSVSRTSFDWGVKVPGSEDHVMYVWVDALTNYITGLG
YPDDTELWRKHWPADLHLIGKDIVRFHTIYWPAFLMSAGLPVPKQVFGHGFVLKDGRKESKSLGNVTDPHDLADFYGVDA
LRYFLMREVSFGSDGNWSHEAIVTRVNAELANSFGNLAQRTLSMIHKNMDGSLEKFEARGEDKALLQTVLTACRESLPRE
FEKLAFSVGIDEWIKAVYACNQYVDEQAPWALRKTDPERMKAVLQTLLMAIHDLAIAIQPVIPEKAGALLDDLGVPTDRR
TYDHISGEGWFNQLVAEGFTVPKPTPAFPRLEMPESEDA

Sequences:

>Translated_519_residues
MSEPFYITTAISYPNGRPHIGHAYEAIAADVIARFQRQRGLDVRFQTGTDEHGLKMARKAEEQGKSPRDLADEMSGYFKA
MCDALNVSCDRFIRTVDDDHHRASQAIWRAMEAAGDLYLDRYEGWYSVRDEAYYDESELVDGEGDEKLSPQGTPVEWTVE
ESWFFRLSKYEDKLLKLLESPGFLEPASRRNEMVAFVKQGLRDLSVSRTSFDWGVKVPGSEDHVMYVWVDALTNYITGLG
YPDDTELWRKHWPADLHLIGKDIVRFHTIYWPAFLMSAGLPVPKQVFGHGFVLKDGRKESKSLGNVTDPHDLADFYGVDA
LRYFLMREVSFGSDGNWSHEAIVTRVNAELANSFGNLAQRTLSMIHKNMDGSLEKFEARGEDKALLQTVLTACRESLPRE
FEKLAFSVGIDEWIKAVYACNQYVDEQAPWALRKTDPERMKAVLQTLLMAIHDLAIAIQPVIPEKAGALLDDLGVPTDRR
TYDHISGEGWFNQLVAEGFTVPKPTPAFPRLEMPESEDA
>Mature_518_residues
SEPFYITTAISYPNGRPHIGHAYEAIAADVIARFQRQRGLDVRFQTGTDEHGLKMARKAEEQGKSPRDLADEMSGYFKAM
CDALNVSCDRFIRTVDDDHHRASQAIWRAMEAAGDLYLDRYEGWYSVRDEAYYDESELVDGEGDEKLSPQGTPVEWTVEE
SWFFRLSKYEDKLLKLLESPGFLEPASRRNEMVAFVKQGLRDLSVSRTSFDWGVKVPGSEDHVMYVWVDALTNYITGLGY
PDDTELWRKHWPADLHLIGKDIVRFHTIYWPAFLMSAGLPVPKQVFGHGFVLKDGRKESKSLGNVTDPHDLADFYGVDAL
RYFLMREVSFGSDGNWSHEAIVTRVNAELANSFGNLAQRTLSMIHKNMDGSLEKFEARGEDKALLQTVLTACRESLPREF
EKLAFSVGIDEWIKAVYACNQYVDEQAPWALRKTDPERMKAVLQTLLMAIHDLAIAIQPVIPEKAGALLDDLGVPTDRRT
YDHISGEGWFNQLVAEGFTVPKPTPAFPRLEMPESEDA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=36.6666666666667, Blast_Score=311, Evalue=8e-85,
Organism=Homo sapiens, GI14043022, Length=521, Percent_Identity=25.3358925143954, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1788432, Length=512, Percent_Identity=29.1015625, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1786861, Length=182, Percent_Identity=25.8241758241758, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=495, Percent_Identity=33.7373737373737, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI71992781, Length=389, Percent_Identity=32.6478149100257, Blast_Score=207, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17540794, Length=517, Percent_Identity=24.9516441005803, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321610, Length=526, Percent_Identity=31.9391634980989, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321704, Length=550, Percent_Identity=24.1818181818182, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21358483, Length=498, Percent_Identity=37.14859437751, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24655311, Length=554, Percent_Identity=24.5487364620939, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21355409, Length=180, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58894; Mature: 58763

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPFYITTAISYPNGRPHIGHAYEAIAADVIARFQRQRGLDVRFQTGTDEHGLKMARKA
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
EEQGKSPRDLADEMSGYFKAMCDALNVSCDRFIRTVDDDHHRASQAIWRAMEAAGDLYLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RYEGWYSVRDEAYYDESELVDGEGDEKLSPQGTPVEWTVEESWFFRLSKYEDKLLKLLES
HHCCCHHCCCHHHCCHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHC
PGFLEPASRRNEMVAFVKQGLRDLSVSRTSFDWGVKVPGSEDHVMYVWVDALTNYITGLG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHCCC
YPDDTELWRKHWPADLHLIGKDIVRFHTIYWPAFLMSAGLPVPKQVFGHGFVLKDGRKES
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCHHH
KSLGNVTDPHDLADFYGVDALRYFLMREVSFGSDGNWSHEAIVTRVNAELANSFGNLAQR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLSMIHKNMDGSLEKFEARGEDKALLQTVLTACRESLPREFEKLAFSVGIDEWIKAVYAC
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NQYVDEQAPWALRKTDPERMKAVLQTLLMAIHDLAIAIQPVIPEKAGALLDDLGVPTDRR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
TYDHISGEGWFNQLVAEGFTVPKPTPAFPRLEMPESEDA
CHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEPFYITTAISYPNGRPHIGHAYEAIAADVIARFQRQRGLDVRFQTGTDEHGLKMARKA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHH
EEQGKSPRDLADEMSGYFKAMCDALNVSCDRFIRTVDDDHHRASQAIWRAMEAAGDLYLD
HHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RYEGWYSVRDEAYYDESELVDGEGDEKLSPQGTPVEWTVEESWFFRLSKYEDKLLKLLES
HHCCCHHCCCHHHCCHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHC
PGFLEPASRRNEMVAFVKQGLRDLSVSRTSFDWGVKVPGSEDHVMYVWVDALTNYITGLG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCEEEEEHHHHHHHHHCCC
YPDDTELWRKHWPADLHLIGKDIVRFHTIYWPAFLMSAGLPVPKQVFGHGFVLKDGRKES
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCHHH
KSLGNVTDPHDLADFYGVDALRYFLMREVSFGSDGNWSHEAIVTRVNAELANSFGNLAQR
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TLSMIHKNMDGSLEKFEARGEDKALLQTVLTACRESLPREFEKLAFSVGIDEWIKAVYAC
HHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NQYVDEQAPWALRKTDPERMKAVLQTLLMAIHDLAIAIQPVIPEKAGALLDDLGVPTDRR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
TYDHISGEGWFNQLVAEGFTVPKPTPAFPRLEMPESEDA
CHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]