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The map label for this gene is tmk [H]

Identifier: 85374070

GI number: 85374070

Start: 1292811

End: 1293449

Strand: Direct

Name: tmk [H]

Synonym: ELI_06215

Alternate gene names: 85374070

Gene position: 1292811-1293449 (Clockwise)

Preceding gene: 85374069

Following gene: 85374071

Centisome position: 42.35

GC content: 65.1

Gene sequence:

>639_bases
ATGACGCGAGGGCGGTTCATCGCGCTCGAAGGCGGGGAGGGCACGGGCAAGTCCACCCAGGCGCGCTTACTCGCCGAGGC
CTTGAGCACGCGTGGTATCGAATGCGTCGTCACGCGTGAACCCGGCGGCACGGAAGGGGCCGAAGCGATCCGCCGATTGC
TGCTCGATCCGCCAGGAGATGGCTGGGGACCGCGAGCCGAAGCCCTGCTGTTCGCCGCTGCGCGGTCGGACCATGTCGAA
AAGCTGATATTGCCAGCACTCGAGCGCGGCGCGTGGGTCGTTTCGGATCGCTTTCTCGATTCCAGTCGCGCTTACCAGGG
CGGGGCAGGGGGCATCGGCGATCAAGCGGTGCGCACCTTGCACGATATCGGCAGTGGCGGGCTTCGCCCGGATCTGACCG
TCCTGATCGAAGTCGAACCCGCGACAATCGCCGAACGCTTGGCTGATCGCGACGGAAATCGCTCGGACGCGATCGGGGGG
CGGAGCGCGCAATATCACGCCGACGTTGCTGCGTCGTTCAAGCGGTTTGCAGCGGAAGACCCGGGAGGCTTTGCCGTAGT
CGATGGTGAGGGCACGCCCGATGAGGTCCACTCGCGGATTCTAGAAGCAATTGAGCCCCTTATCGGAGGCGAACTCTGA

Upstream 100 bases:

>100_bases
ACGGGCGCGAAGCCGCACGGGTGCCCCTGGCGGCCAAAAAGGCCGTAGCGAAAGCCGGGCCTCTTCAGCGGATTGCCAAT
GCCGTGCGAGCCTTGTGGAC

Downstream 100 bases:

>100_bases
TGCGGCTCACGCATGCCGAAATCCAGCGCGAGTGGGATGCGGCACTGGCTGGCCCGCGAATGCATCACGGATGGATTCTT
GCCGGGCGGCAGGGGCTTGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MTRGRFIALEGGEGTGKSTQARLLAEALSTRGIECVVTREPGGTEGAEAIRRLLLDPPGDGWGPRAEALLFAAARSDHVE
KLILPALERGAWVVSDRFLDSSRAYQGGAGGIGDQAVRTLHDIGSGGLRPDLTVLIEVEPATIAERLADRDGNRSDAIGG
RSAQYHADVAASFKRFAAEDPGGFAVVDGEGTPDEVHSRILEAIEPLIGGEL

Sequences:

>Translated_212_residues
MTRGRFIALEGGEGTGKSTQARLLAEALSTRGIECVVTREPGGTEGAEAIRRLLLDPPGDGWGPRAEALLFAAARSDHVE
KLILPALERGAWVVSDRFLDSSRAYQGGAGGIGDQAVRTLHDIGSGGLRPDLTVLIEVEPATIAERLADRDGNRSDAIGG
RSAQYHADVAASFKRFAAEDPGGFAVVDGEGTPDEVHSRILEAIEPLIGGEL
>Mature_211_residues
TRGRFIALEGGEGTGKSTQARLLAEALSTRGIECVVTREPGGTEGAEAIRRLLLDPPGDGWGPRAEALLFAAARSDHVEK
LILPALERGAWVVSDRFLDSSRAYQGGAGGIGDQAVRTLHDIGSGGLRPDLTVLIEVEPATIAERLADRDGNRSDAIGGR
SAQYHADVAASFKRFAAEDPGGFAVVDGEGTPDEVHSRILEAIEPLIGGEL

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=184, Percent_Identity=40.2173913043478, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 22263; Mature: 22132

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGRFIALEGGEGTGKSTQARLLAEALSTRGIECVVTREPGGTEGAEAIRRLLLDPPGD
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCC
GWGPRAEALLFAAARSDHVEKLILPALERGAWVVSDRFLDSSRAYQGGAGGIGDQAVRTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCCCCHHHHHHHH
HDIGSGGLRPDLTVLIEVEPATIAERLADRDGNRSDAIGGRSAQYHADVAASFKRFAAED
HHHCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PGGFAVVDGEGTPDEVHSRILEAIEPLIGGEL
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRGRFIALEGGEGTGKSTQARLLAEALSTRGIECVVTREPGGTEGAEAIRRLLLDPPGD
CCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCC
GWGPRAEALLFAAARSDHVEKLILPALERGAWVVSDRFLDSSRAYQGGAGGIGDQAVRTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCCCCCCCCCCCCCHHHHHHHH
HDIGSGGLRPDLTVLIEVEPATIAERLADRDGNRSDAIGGRSAQYHADVAASFKRFAAED
HHHCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PGGFAVVDGEGTPDEVHSRILEAIEPLIGGEL
CCCEEEECCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA