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The map label for this gene is dacA [H]

Identifier: 85374069

GI number: 85374069

Start: 1291771

End: 1292814

Strand: Direct

Name: dacA [H]

Synonym: ELI_06210

Alternate gene names: 85374069

Gene position: 1291771-1292814 (Clockwise)

Preceding gene: 85374068

Following gene: 85374070

Centisome position: 42.32

GC content: 62.74

Gene sequence:

>1044_bases
ATGCTGGTCGATATGGCCAGCGGGCAGACACTCTATGCGCGCAACCCCGACCGCCGCTTCGTCCCTGCTTCGATTACGAA
GGTGATGACGGCCTACACGGCGTTCGAGCTGATGAAGCAGGGCAAGCTCGCACCGGGTACCCGCTTCACATTCTCCGAAG
CGGCAGCCGACGAATGGTATCGCACCGGGTCCACGATGTTTCTCGACCGGGGCAGCGAAACTGCCGTAACCGACCTCCTG
ATGGGTATTACTACGGTTTCGGCGAACGATGGGAGCATCGTGCTTGCCGAGGGTGCTGCCGGCTCGGTCGACAACTGGCT
CGACCTGATGAACGCGCACGCCGACGCGCTCGGCATGCACCAAAGTCATTTCGGCACGCCGAATGGATGGCCGGACGAAG
GGCGAACTTTCACCACGGCGCGCGACCTCGTGACCTTGGCCAAGGCCCTGATTACCGAACATCCGGGGTTGTATGCACGC
TACTTTGGCCATCGCGGCCTGCGGACCAATGGGATTGCCCAGGATAATTACGACCCGATTACCGGCCGTATCGAGGGCGC
AGACGGGATCAAGACAGGCTTCACCAACCAGGCAGGCTGGGGATTTCTCGGCAGCGCCGAACGCGACGGTCGGCGCCTGG
TCATGGTCGTCGCCGCCAGTCCGGGAATGCGCAGTCGTGACGAGACTGCACGGGCCCTGATGGAATGGGGCTTCGGTGCT
TTCGACAGCCGTATGCTGTTCCGTTCGGGCGACACTGTGGGGCACGCGAGGGTGCAGGACGGCGCGGAAGCAGCGGTCCC
GTTGATGGCGGACCGAGACATCCCGGTTGCGCTACCAGAGAATGCGAACGCTGAAATCACCTTGCGCATCGCTTACGAAG
GGCCTTTGAGGGCACCTGTCGAGCAAGGGGAGAAGGTGGCGCAACTGGCGATATTTGTAGACGGGCGCGAAGCCGCACGG
GTGCCCCTGGCGGCCAAAAAGGCCGTAGCGAAAGCCGGGCCTCTTCAGCGGATTGCCAATGCCGTGCGAGCCTTGTGGAC
ATGA

Upstream 100 bases:

>100_bases
TCGGGTGAGGATCGATTTCAGGAGTGCATTGGCTGTGTGCGGGCTTGCAATTGCGAGCCCTGCCCATGGCGCACCGGTTC
CCGGCGAAGTCCCTATTGCG

Downstream 100 bases:

>100_bases
CGCGAGGGCGGTTCATCGCGCTCGAAGGCGGGGAGGGCACGGGCAAGTCCACCCAGGCGCGCTTACTCGCCGAGGCCTTG
AGCACGCGTGGTATCGAATG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLVDMASGQTLYARNPDRRFVPASITKVMTAYTAFELMKQGKLAPGTRFTFSEAAADEWYRTGSTMFLDRGSETAVTDLL
MGITTVSANDGSIVLAEGAAGSVDNWLDLMNAHADALGMHQSHFGTPNGWPDEGRTFTTARDLVTLAKALITEHPGLYAR
YFGHRGLRTNGIAQDNYDPITGRIEGADGIKTGFTNQAGWGFLGSAERDGRRLVMVVAASPGMRSRDETARALMEWGFGA
FDSRMLFRSGDTVGHARVQDGAEAAVPLMADRDIPVALPENANAEITLRIAYEGPLRAPVEQGEKVAQLAIFVDGREAAR
VPLAAKKAVAKAGPLQRIANAVRALWT

Sequences:

>Translated_347_residues
MLVDMASGQTLYARNPDRRFVPASITKVMTAYTAFELMKQGKLAPGTRFTFSEAAADEWYRTGSTMFLDRGSETAVTDLL
MGITTVSANDGSIVLAEGAAGSVDNWLDLMNAHADALGMHQSHFGTPNGWPDEGRTFTTARDLVTLAKALITEHPGLYAR
YFGHRGLRTNGIAQDNYDPITGRIEGADGIKTGFTNQAGWGFLGSAERDGRRLVMVVAASPGMRSRDETARALMEWGFGA
FDSRMLFRSGDTVGHARVQDGAEAAVPLMADRDIPVALPENANAEITLRIAYEGPLRAPVEQGEKVAQLAIFVDGREAAR
VPLAAKKAVAKAGPLQRIANAVRALWT
>Mature_347_residues
MLVDMASGQTLYARNPDRRFVPASITKVMTAYTAFELMKQGKLAPGTRFTFSEAAADEWYRTGSTMFLDRGSETAVTDLL
MGITTVSANDGSIVLAEGAAGSVDNWLDLMNAHADALGMHQSHFGTPNGWPDEGRTFTTARDLVTLAKALITEHPGLYAR
YFGHRGLRTNGIAQDNYDPITGRIEGADGIKTGFTNQAGWGFLGSAERDGRRLVMVVAASPGMRSRDETARALMEWGFGA
FDSRMLFRSGDTVGHARVQDGAEAAVPLMADRDIPVALPENANAEITLRIAYEGPLRAPVEQGEKVAQLAIFVDGREAAR
VPLAAKKAVAKAGPLQRIANAVRALWT

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=359, Percent_Identity=29.2479108635098, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI87082022, Length=348, Percent_Identity=29.0229885057471, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787062, Length=350, Percent_Identity=32.2857142857143, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 37377; Mature: 37377

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVDMASGQTLYARNPDRRFVPASITKVMTAYTAFELMKQGKLAPGTRFTFSEAAADEWY
CEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
RTGSTMFLDRGSETAVTDLLMGITTVSANDGSIVLAEGAAGSVDNWLDLMNAHADALGMH
HCCCEEEEECCCCHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCH
QSHFGTPNGWPDEGRTFTTARDLVTLAKALITEHPGLYARYFGHRGLRTNGIAQDNYDPI
HHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCC
TGRIEGADGIKTGFTNQAGWGFLGSAERDGRRLVMVVAASPGMRSRDETARALMEWGFGA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
FDSRMLFRSGDTVGHARVQDGAEAAVPLMADRDIPVALPENANAEITLRIAYEGPLRAPV
CCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCEEEEEEEECCCCCCCH
EQGEKVAQLAIFVDGREAARVPLAAKKAVAKAGPLQRIANAVRALWT
HHCCCEEEEEEEECCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVDMASGQTLYARNPDRRFVPASITKVMTAYTAFELMKQGKLAPGTRFTFSEAAADEWY
CEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH
RTGSTMFLDRGSETAVTDLLMGITTVSANDGSIVLAEGAAGSVDNWLDLMNAHADALGMH
HCCCEEEEECCCCHHHHHHHHHHEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCH
QSHFGTPNGWPDEGRTFTTARDLVTLAKALITEHPGLYARYFGHRGLRTNGIAQDNYDPI
HHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCC
TGRIEGADGIKTGFTNQAGWGFLGSAERDGRRLVMVVAASPGMRSRDETARALMEWGFGA
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
FDSRMLFRSGDTVGHARVQDGAEAAVPLMADRDIPVALPENANAEITLRIAYEGPLRAPV
CCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCCEEEEEEEECCCCCCCH
EQGEKVAQLAIFVDGREAARVPLAAKKAVAKAGPLQRIANAVRALWT
HHCCCEEEEEEEECCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]