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The map label for this gene is pbpF [H]

Identifier: 85373362

GI number: 85373362

Start: 572723

End: 574852

Strand: Direct

Name: pbpF [H]

Synonym: ELI_02675

Alternate gene names: 85373362

Gene position: 572723-574852 (Clockwise)

Preceding gene: 85373361

Following gene: 85373372

Centisome position: 18.76

GC content: 64.51

Gene sequence:

>2130_bases
ATGAATTCCCCCTGGTTCCGCAAACTGACCCCTTGGCGGCGCGATCCCGGTCGGCCGCAGGGTGCTTACGCGGGCTATTT
CTCGCGCGCTGACCAAGAGCCGCTCTATCGCTCCGGTTCGCCCCAACCCGATTTCGATCGCTGGGACAACCGCCTCGATC
GGCTTGACGGAGCGGTCGAGCGCGAGGAGCGGCGCAAGCAGCGGTGGTGGCAGCCGGCCTATTGGCGCGAGCGGCGCAAG
CGCTGGTGGATTGTAAGATTTTTTGCCGCTGCGTTGATGGCTTTCCTTGCTCTGATCGCGTTTCTTGCGCTGACGACGCC
GCTCGACAAATCGCTGGAGCCGGTCGTTCCGCCGCAGATGACGCTGCTGGCTGCGGACGGAACTCCCATTGCCCGCAATG
GGGCGATCGTCGGCGAGCCAGTGAGCGTCGAAACCCTGCCGCCGCATGTGGTGGAAGCCTTTCTCGCGATCGAGGACCGC
CGGTTCTACGATCACTGGGGCGTCGATCCGCGCGGCATCGCACGCGCTGCATGGACCGGTGTTGGCGGCGGGAGCACTAT
TACCCAGCAGCTGGCGAAATTCACGTTTCTCAGTCCCGAGCGGACACTGACGCGCAAGGCGCGCGAAGCGCTCATCGCAT
TCTGGCTCGAGACCTGGCTGACCAAGGACGAGATTCTCGAACGCTACCTTTCCAACACCTATTTCGGCGACAATGTTTAT
GGCCTGCGCGCGGCGAGCCTGCACTATTTCTACCGCCAGCCCGAGAAACTGACGCTGGAACAGGCGGCGATGCTGGCCGG
ACTGGTCAAGGCACCCTCGGCCTTGCGGCCCACCAAGCACTATGCGCGCGCCGAAGAGCGGATGAAGCTGGTCGTCGCAT
CCATGGTTGCAGCCGGCTATCTCACCGAAGCCGAGGCGGCGCGCGTTTCTGCGCCAAAGCTCGACGTGCGGACCAGATCA
GACCTGCCGACCGGCACCTATTTTGCCGATTGGGCGCTGCCGATGCTGCGTCGCGATGCCGAAACGGGCTACGAAGCGCA
GACGATTACGACTACGCTCGATGCACGGTTGCAAGGTATCGCGAGCCGCATCGTCCAGCGCGCCAATCTGGGCGGGGCGC
AGGCGGCACTGGTGGCGATGCGCTCCAATGGCGAAGTTGTCGCGATGGTCGGCGGAAAGGATTATCAGGAAAGCCCGTTC
AACCGCGTGACGCAGGCGAAACGGCAACCGGGATCGACCTTCAAGACCTTCGTCTGGCTGGCGGCGCTCGAAGCAGGGGC
CGATCCGACCGATCCCGTCGACAATACCGAGATCACGCAAGGCAGCTATCGTCCGCGCAACTATGGCGAACGCTATTCTG
GTTCTCTCACTTTGGAGCAGGCCTTCGCACGATCCAGCAATGTGGCGGCGGTGCGCCTTTACAATTCGGTGGGCGACGAA
GCGGTAATCCGCACCGCGCGGCGGCTCGGGGTGACTTCGCCGCTTCCACCGGGCGATCCCAGCATGGCGCTCGGCACATC
GACCATGACGCTGCTAGAATTGACCGCCGCCTATGCCGGCATCGCCGCGAACGCTTATCCCGTGCGCCCAACGCCGCTGC
CGGTGCAGGAGCAGGGCTGGTGGGAATGGCTGTGGGACGGACCCGAGAGCTTCTCCGGCCGGGTCCATGACGACATGCAA
CAGATGCTGCGCGCTGCCGTGAATAGTGGCACCGGGCGCGCTGCCATGCTGGCGGGGCCGAACTACGGCAAGACCGGTAC
CAGCCAGGACAATCGCGACGCCCTGTTTGTGGGCTATGCCGGAGATCTCGTGGTCGGCGTGTGGATCGGCAATGATGACA
ACAGTCCACTCAATGGCGTGACCGGGGGTGGGCTCCCGGCGCGGATGTGGCGCGATTTCATGCGTCAGGCGCAGGGGGCG
TCATCCGCAGGTGACACCCCGCGGGCCAATCCCTCCGGACCCGTGCAGCCGATGGACGTCCCGGAACTGGAAGACGTGCC
TCTGGGCGAGGGCGTGCGTCTGGAGGACGGCGGGCTGAATATCGAAACCGAGATCGAGGGCATTCCAGTCGATATTCGCG
TCACGCCAGACGGCGTCGAAATGGCGCCGGGCAGCGAGCCCGACGGCTAG

Upstream 100 bases:

>100_bases
TGGCGGAATACTACCAGCGCGTTTTCGGGGAAGACCTGCCCGAGAGCGTGGTCAAGGCTTGAGACTGGCAATCGCTGTGT
TATCCTGCTAATACAGCCGG

Downstream 100 bases:

>100_bases
CCTGCCGATTCCGCAAGCAGCACGTTCATAATCGCCGTCGCGCGCGGGCGCTCGCGATCATCCTGCCAGCACTCGACCGC
GATATTGGCATTGCGCCGGC

Product: penicillin-binding protein, 1A family protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MNSPWFRKLTPWRRDPGRPQGAYAGYFSRADQEPLYRSGSPQPDFDRWDNRLDRLDGAVEREERRKQRWWQPAYWRERRK
RWWIVRFFAAALMAFLALIAFLALTTPLDKSLEPVVPPQMTLLAADGTPIARNGAIVGEPVSVETLPPHVVEAFLAIEDR
RFYDHWGVDPRGIARAAWTGVGGGSTITQQLAKFTFLSPERTLTRKAREALIAFWLETWLTKDEILERYLSNTYFGDNVY
GLRAASLHYFYRQPEKLTLEQAAMLAGLVKAPSALRPTKHYARAEERMKLVVASMVAAGYLTEAEAARVSAPKLDVRTRS
DLPTGTYFADWALPMLRRDAETGYEAQTITTTLDARLQGIASRIVQRANLGGAQAALVAMRSNGEVVAMVGGKDYQESPF
NRVTQAKRQPGSTFKTFVWLAALEAGADPTDPVDNTEITQGSYRPRNYGERYSGSLTLEQAFARSSNVAAVRLYNSVGDE
AVIRTARRLGVTSPLPPGDPSMALGTSTMTLLELTAAYAGIAANAYPVRPTPLPVQEQGWWEWLWDGPESFSGRVHDDMQ
QMLRAAVNSGTGRAAMLAGPNYGKTGTSQDNRDALFVGYAGDLVVGVWIGNDDNSPLNGVTGGGLPARMWRDFMRQAQGA
SSAGDTPRANPSGPVQPMDVPELEDVPLGEGVRLEDGGLNIETEIEGIPVDIRVTPDGVEMAPGSEPDG

Sequences:

>Translated_709_residues
MNSPWFRKLTPWRRDPGRPQGAYAGYFSRADQEPLYRSGSPQPDFDRWDNRLDRLDGAVEREERRKQRWWQPAYWRERRK
RWWIVRFFAAALMAFLALIAFLALTTPLDKSLEPVVPPQMTLLAADGTPIARNGAIVGEPVSVETLPPHVVEAFLAIEDR
RFYDHWGVDPRGIARAAWTGVGGGSTITQQLAKFTFLSPERTLTRKAREALIAFWLETWLTKDEILERYLSNTYFGDNVY
GLRAASLHYFYRQPEKLTLEQAAMLAGLVKAPSALRPTKHYARAEERMKLVVASMVAAGYLTEAEAARVSAPKLDVRTRS
DLPTGTYFADWALPMLRRDAETGYEAQTITTTLDARLQGIASRIVQRANLGGAQAALVAMRSNGEVVAMVGGKDYQESPF
NRVTQAKRQPGSTFKTFVWLAALEAGADPTDPVDNTEITQGSYRPRNYGERYSGSLTLEQAFARSSNVAAVRLYNSVGDE
AVIRTARRLGVTSPLPPGDPSMALGTSTMTLLELTAAYAGIAANAYPVRPTPLPVQEQGWWEWLWDGPESFSGRVHDDMQ
QMLRAAVNSGTGRAAMLAGPNYGKTGTSQDNRDALFVGYAGDLVVGVWIGNDDNSPLNGVTGGGLPARMWRDFMRQAQGA
SSAGDTPRANPSGPVQPMDVPELEDVPLGEGVRLEDGGLNIETEIEGIPVDIRVTPDGVEMAPGSEPDG
>Mature_709_residues
MNSPWFRKLTPWRRDPGRPQGAYAGYFSRADQEPLYRSGSPQPDFDRWDNRLDRLDGAVEREERRKQRWWQPAYWRERRK
RWWIVRFFAAALMAFLALIAFLALTTPLDKSLEPVVPPQMTLLAADGTPIARNGAIVGEPVSVETLPPHVVEAFLAIEDR
RFYDHWGVDPRGIARAAWTGVGGGSTITQQLAKFTFLSPERTLTRKAREALIAFWLETWLTKDEILERYLSNTYFGDNVY
GLRAASLHYFYRQPEKLTLEQAAMLAGLVKAPSALRPTKHYARAEERMKLVVASMVAAGYLTEAEAARVSAPKLDVRTRS
DLPTGTYFADWALPMLRRDAETGYEAQTITTTLDARLQGIASRIVQRANLGGAQAALVAMRSNGEVVAMVGGKDYQESPF
NRVTQAKRQPGSTFKTFVWLAALEAGADPTDPVDNTEITQGSYRPRNYGERYSGSLTLEQAFARSSNVAAVRLYNSVGDE
AVIRTARRLGVTSPLPPGDPSMALGTSTMTLLELTAAYAGIAANAYPVRPTPLPVQEQGWWEWLWDGPESFSGRVHDDMQ
QMLRAAVNSGTGRAAMLAGPNYGKTGTSQDNRDALFVGYAGDLVVGVWIGNDDNSPLNGVTGGGLPARMWRDFMRQAQGA
SSAGDTPRANPSGPVQPMDVPELEDVPLGEGVRLEDGGLNIETEIEGIPVDIRVTPDGVEMAPGSEPDG

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=530, Percent_Identity=31.8867924528302, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI1786343, Length=521, Percent_Identity=32.6295585412668, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI87082258, Length=263, Percent_Identity=39.5437262357414, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=33.9393939393939, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 78098; Mature: 78098

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPWFRKLTPWRRDPGRPQGAYAGYFSRADQEPLYRSGSPQPDFDRWDNRLDRLDGAVE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
REERRKQRWWQPAYWRERRKRWWIVRFFAAALMAFLALIAFLALTTPLDKSLEPVVPPQM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
TLLAADGTPIARNGAIVGEPVSVETLPPHVVEAFLAIEDRRFYDHWGVDPRGIARAAWTG
EEEEECCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCC
VGGGSTITQQLAKFTFLSPERTLTRKAREALIAFWLETWLTKDEILERYLSNTYFGDNVY
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
GLRAASLHYFYRQPEKLTLEQAAMLAGLVKAPSALRPTKHYARAEERMKLVVASMVAAGY
EEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LTEAEAARVSAPKLDVRTRSDLPTGTYFADWALPMLRRDAETGYEAQTITTTLDARLQGI
HHHHHHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
ASRIVQRANLGGAQAALVAMRSNGEVVAMVGGKDYQESPFNRVTQAKRQPGSTFKTFVWL
HHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
AALEAGADPTDPVDNTEITQGSYRPRNYGERYSGSLTLEQAFARSSNVAAVRLYNSVGDE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHCCCCEEEEEEECCCCHH
AVIRTARRLGVTSPLPPGDPSMALGTSTMTLLELTAAYAGIAANAYPVRPTPLPVQEQGW
HHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WEWLWDGPESFSGRVHDDMQQMLRAAVNSGTGRAAMLAGPNYGKTGTSQDNRDALFVGYA
EEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
GDLVVGVWIGNDDNSPLNGVTGGGLPARMWRDFMRQAQGASSAGDTPRANPSGPVQPMDV
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PELEDVPLGEGVRLEDGGLNIETEIEGIPVDIRVTPDGVEMAPGSEPDG
CCCCCCCCCCCEEEECCCCEEEEECCCEEEEEEECCCCCEECCCCCCCC
>Mature Secondary Structure
MNSPWFRKLTPWRRDPGRPQGAYAGYFSRADQEPLYRSGSPQPDFDRWDNRLDRLDGAVE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
REERRKQRWWQPAYWRERRKRWWIVRFFAAALMAFLALIAFLALTTPLDKSLEPVVPPQM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
TLLAADGTPIARNGAIVGEPVSVETLPPHVVEAFLAIEDRRFYDHWGVDPRGIARAAWTG
EEEEECCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCC
VGGGSTITQQLAKFTFLSPERTLTRKAREALIAFWLETWLTKDEILERYLSNTYFGDNVY
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
GLRAASLHYFYRQPEKLTLEQAAMLAGLVKAPSALRPTKHYARAEERMKLVVASMVAAGY
EEHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LTEAEAARVSAPKLDVRTRSDLPTGTYFADWALPMLRRDAETGYEAQTITTTLDARLQGI
HHHHHHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHH
ASRIVQRANLGGAQAALVAMRSNGEVVAMVGGKDYQESPFNRVTQAKRQPGSTFKTFVWL
HHHHHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
AALEAGADPTDPVDNTEITQGSYRPRNYGERYSGSLTLEQAFARSSNVAAVRLYNSVGDE
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHCCCCEEEEEEECCCCHH
AVIRTARRLGVTSPLPPGDPSMALGTSTMTLLELTAAYAGIAANAYPVRPTPLPVQEQGW
HHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
WEWLWDGPESFSGRVHDDMQQMLRAAVNSGTGRAAMLAGPNYGKTGTSQDNRDALFVGYA
EEEECCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEC
GDLVVGVWIGNDDNSPLNGVTGGGLPARMWRDFMRQAQGASSAGDTPRANPSGPVQPMDV
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
PELEDVPLGEGVRLEDGGLNIETEIEGIPVDIRVTPDGVEMAPGSEPDG
CCCCCCCCCCCEEEECCCCEEEEECCCEEEEEEECCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]