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The map label for this gene is vanX [H]

Identifier: 84625532

GI number: 84625532

Start: 4376008

End: 4376754

Strand: Reverse

Name: vanX [H]

Synonym: XOO_3875

Alternate gene names: 84625532

Gene position: 4376754-4376008 (Counterclockwise)

Preceding gene: 84625533

Following gene: 84625531

Centisome position: 88.59

GC content: 63.32

Gene sequence:

>747_bases
ATGAAAGAAACCCTGCTATTTCGGAAAGGGCGCTGCTGGCGGCAGCTTTTTCTTCAAGGCGCTCTGCTGATGATCGCCAT
GGCGCCATCCGCCCTGGTCGCCAGCGACGTGCATGTGTCCTCGGCAAAGACCGCCGCAGAGGCCGGCCTTGTGGACGTAC
ACGCCCTTGCGCCGGACATTGCGTTGGACATGCGTCATGCAAGCAGCAACAACTTCACCGGGCAGGTCGTGCCGGGATGT
AACGCGCCGCGGTGTTATCTGCTGCAGCCAGCTGCCGAAGCGTTGGCGCGGGTGGCGCATACGCTTGAGGCCGAAGGGGA
CAGATTGCAAGTGTCCGACTGCTATCGGCCGGTGCGGTCTGTGAAAGCATTCGTGGCCTGGGCGGCGGATCTGCAAAATC
AGACGACCAGGGCGCAGTACTACCCGCGGGTGGACAAGCGTGCGCTGCTGGGCGATTACATTGCTCCGACCTCCGGGCAT
CGCCGTGGCGCGACCCTGGATCTGGGTTTGCTGCAGTGCCGGTCGGCTGCGTGCCAGGCAGTGGACATGGGCACCGGTTT
CGATGTCTTCGATGCGCGTGCGCACACCGATGCGCCAGACATCAGCGTAGGGCAACGCGCACACCGCCAGCGCCTGCGGC
GTGCGATGGCGGCCGAAGGATTTGCCAACTATCCGATGGAGTGGTGGCATTTCACGTTTCGTCCCGAGCCCACGCCTGAT
ACGGCCTACGCTGTTTCGGTGGACTGA

Upstream 100 bases:

>100_bases
ACCGCTAGGCGCATGGCGGTCGAGCCACGCCGTGTCTGGTGGCGAAGCAGGCTCAGTGTGATCCATCGCGCGGACCCTGC
ATCTTGCAAGGAAACGACAG

Downstream 100 bases:

>100_bases
CGGCGACCGATGAATAGCTGCGCCGGCGCCGACCATCAGGTGGTGCTCGCAATCGGTAGCCCGCTTGCGGTAGGTGCGCA
TTGCAACGCAGAAACGGCCA

Product: D-alanyl-D-alanine dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKETLLFRKGRCWRQLFLQGALLMIAMAPSALVASDVHVSSAKTAAEAGLVDVHALAPDIALDMRHASSNNFTGQVVPGC
NAPRCYLLQPAAEALARVAHTLEAEGDRLQVSDCYRPVRSVKAFVAWAADLQNQTTRAQYYPRVDKRALLGDYIAPTSGH
RRGATLDLGLLQCRSAACQAVDMGTGFDVFDARAHTDAPDISVGQRAHRQRLRRAMAAEGFANYPMEWWHFTFRPEPTPD
TAYAVSVD

Sequences:

>Translated_248_residues
MKETLLFRKGRCWRQLFLQGALLMIAMAPSALVASDVHVSSAKTAAEAGLVDVHALAPDIALDMRHASSNNFTGQVVPGC
NAPRCYLLQPAAEALARVAHTLEAEGDRLQVSDCYRPVRSVKAFVAWAADLQNQTTRAQYYPRVDKRALLGDYIAPTSGH
RRGATLDLGLLQCRSAACQAVDMGTGFDVFDARAHTDAPDISVGQRAHRQRLRRAMAAEGFANYPMEWWHFTFRPEPTPD
TAYAVSVD
>Mature_248_residues
MKETLLFRKGRCWRQLFLQGALLMIAMAPSALVASDVHVSSAKTAAEAGLVDVHALAPDIALDMRHASSNNFTGQVVPGC
NAPRCYLLQPAAEALARVAHTLEAEGDRLQVSDCYRPVRSVKAFVAWAADLQNQTTRAQYYPRVDKRALLGDYIAPTSGH
RRGATLDLGLLQCRSAACQAVDMGTGFDVFDARAHTDAPDISVGQRAHRQRLRRAMAAEGFANYPMEWWHFTFRPEPTPD
TAYAVSVD

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 27213; Mature: 27213

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETLLFRKGRCWRQLFLQGALLMIAMAPSALVASDVHVSSAKTAAEAGLVDVHALAPDI
CCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCEEHHHHCCCH
ALDMRHASSNNFTGQVVPGCNAPRCYLLQPAAEALARVAHTLEAEGDRLQVSDCYRPVRS
HHHHHHCCCCCCCEEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
VKAFVAWAADLQNQTTRAQYYPRVDKRALLGDYIAPTSGHRRGATLDLGLLQCRSAACQA
HHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHE
VDMGTGFDVFDARAHTDAPDISVGQRAHRQRLRRAMAAEGFANYPMEWWHFTFRPEPTPD
EECCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCC
TAYAVSVD
CEEEEEEC
>Mature Secondary Structure
MKETLLFRKGRCWRQLFLQGALLMIAMAPSALVASDVHVSSAKTAAEAGLVDVHALAPDI
CCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHCCCEEHHHHCCCH
ALDMRHASSNNFTGQVVPGCNAPRCYLLQPAAEALARVAHTLEAEGDRLQVSDCYRPVRS
HHHHHHCCCCCCCEEECCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
VKAFVAWAADLQNQTTRAQYYPRVDKRALLGDYIAPTSGHRRGATLDLGLLQCRSAACQA
HHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHE
VDMGTGFDVFDARAHTDAPDISVGQRAHRQRLRRAMAAEGFANYPMEWWHFTFRPEPTPD
EECCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCC
TAYAVSVD
CEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]