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The map label for this gene is amiD [H]

Identifier: 84625533

GI number: 84625533

Start: 4376847

End: 4377653

Strand: Reverse

Name: amiD [H]

Synonym: XOO_3876

Alternate gene names: 84625533

Gene position: 4377653-4376847 (Counterclockwise)

Preceding gene: 84625536

Following gene: 84625532

Centisome position: 88.61

GC content: 65.43

Gene sequence:

>807_bases
GTGCTGCCCAGCTTCCCTTTCTCCTCATCGGCCTGGCTGCGTGCAGCCGTCTGCCTGGTCGCGCTGTCGCTGGGCGCCTG
TGCGCACGCACCGGAGCGCAACCCGCTGGCGCGGTGGGTGCCCTCGCCCAATAACGATATGCGGCGACCGATCCTGATCG
TGTTGCATTTCACCGAACAACATTCAGTGCAGCAAAGCGTGAGCACGCTGCGCGGCCGCAACAGCGGTGGTTGGGTCAGT
GCGCACTATCTGATTGGCAAGGATGGGCAGCGCTACCAGTTGGTCAGCGATGCCCAGCGCGCCTGGCACGGCGGTGCCGG
CCGCTGGGGCACCATCACCGACATCAATTCGGCCTCGATCGGTATCGAGCTGGACAACGACGGTAGCGAGCCGTTCGCGC
CGGCACAGATCGACAGCCTGCTGGTGTTGCTGGACGACCTGTGTAACCGCCTGCGTATCCCGCGCACGCAGATAGTTGGC
CACGAAGATGTTGCCCCGACGCGCAGGAACGACCCCGGCCCGTTATTTCCCTGGAAGCGCCTGGCCGAAGCCGGCTTCGG
CCGCTGGCCCGCTGCCGATGCGCCCGCAGCCCCGGCAAGTTTCGACCCATGGCAGGCGCTTGCCATGATCGGCTACAGCA
TCGACGATCCAGCCGCCACCCTGCAGGCGTTTCACCATCACTACCGCGGCCACAGCGCCACCACGCTGGATGCCGAGGAT
CTGCGCATCTTGTCTGCGTTGACCAAAGGCGCGAGAACTTCTAGCCCGCGACCGGCGCTTGAGCCAATCGAAGGCGACAA
CCGCTAG

Upstream 100 bases:

>100_bases
CGCGCAAGCATCAACGCGGCGGATAATGGTGCGGCGGCGACAGCAGCGTGGCCGAGCCGGACACGCCGCGCAGGTCGTCC
GGTCTTCCCAGGAGAAATCC

Downstream 100 bases:

>100_bases
GCGCATGGCGGTCGAGCCACGCCGTGTCTGGTGGCGAAGCAGGCTCAGTGTGATCCATCGCGCGGACCCTGCATCTTGCA
AGGAAACGACAGATGAAAGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MLPSFPFSSSAWLRAAVCLVALSLGACAHAPERNPLARWVPSPNNDMRRPILIVLHFTEQHSVQQSVSTLRGRNSGGWVS
AHYLIGKDGQRYQLVSDAQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCNRLRIPRTQIVG
HEDVAPTRRNDPGPLFPWKRLAEAGFGRWPAADAPAAPASFDPWQALAMIGYSIDDPAATLQAFHHHYRGHSATTLDAED
LRILSALTKGARTSSPRPALEPIEGDNR

Sequences:

>Translated_268_residues
MLPSFPFSSSAWLRAAVCLVALSLGACAHAPERNPLARWVPSPNNDMRRPILIVLHFTEQHSVQQSVSTLRGRNSGGWVS
AHYLIGKDGQRYQLVSDAQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCNRLRIPRTQIVG
HEDVAPTRRNDPGPLFPWKRLAEAGFGRWPAADAPAAPASFDPWQALAMIGYSIDDPAATLQAFHHHYRGHSATTLDAED
LRILSALTKGARTSSPRPALEPIEGDNR
>Mature_268_residues
MLPSFPFSSSAWLRAAVCLVALSLGACAHAPERNPLARWVPSPNNDMRRPILIVLHFTEQHSVQQSVSTLRGRNSGGWVS
AHYLIGKDGQRYQLVSDAQRAWHGGAGRWGTITDINSASIGIELDNDGSEPFAPAQIDSLLVLLDDLCNRLRIPRTQIVG
HEDVAPTRRNDPGPLFPWKRLAEAGFGRWPAADAPAAPASFDPWQALAMIGYSIDDPAATLQAFHHHYRGHSATTLDAED
LRILSALTKGARTSSPRPALEPIEGDNR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=213, Percent_Identity=35.2112676056338, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786300, Length=171, Percent_Identity=32.7485380116959, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29227; Mature: 29227

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSFPFSSSAWLRAAVCLVALSLGACAHAPERNPLARWVPSPNNDMRRPILIVLHFTEQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEEEECHH
HSVQQSVSTLRGRNSGGWVSAHYLIGKDGQRYQLVSDAQRAWHGGAGRWGTITDINSASI
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCEEECCCCEE
GIELDNDGSEPFAPAQIDSLLVLLDDLCNRLRIPRTQIVGHEDVAPTRRNDPGPLFPWKR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
LAEAGFGRWPAADAPAAPASFDPWQALAMIGYSIDDPAATLQAFHHHYRGHSATTLDAED
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECHHH
LRILSALTKGARTSSPRPALEPIEGDNR
HHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLPSFPFSSSAWLRAAVCLVALSLGACAHAPERNPLARWVPSPNNDMRRPILIVLHFTEQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCEEEEEEEECHH
HSVQQSVSTLRGRNSGGWVSAHYLIGKDGQRYQLVSDAQRAWHGGAGRWGTITDINSASI
HHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCEEECCCCEE
GIELDNDGSEPFAPAQIDSLLVLLDDLCNRLRIPRTQIVGHEDVAPTRRNDPGPLFPWKR
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
LAEAGFGRWPAADAPAAPASFDPWQALAMIGYSIDDPAATLQAFHHHYRGHSATTLDAED
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECHHH
LRILSALTKGARTSSPRPALEPIEGDNR
HHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]