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The map label for this gene is lipA [H]

Identifier: 84625387

GI number: 84625387

Start: 4217879

End: 4218892

Strand: Reverse

Name: lipA [H]

Synonym: XOO_3730

Alternate gene names: 84625387

Gene position: 4218892-4217879 (Counterclockwise)

Preceding gene: 84625388

Following gene: 84625386

Centisome position: 85.4

GC content: 64.3

Gene sequence:

>1014_bases
ATGACCCAGCCTACCGCCCGTTCCATCCCCTTGCAGGTCGTCTCCGGCGACACCGCCGCGCCTGCGCCGTTGCAGACCGG
CGTCAAGCAGATCGGTGGCGACAAGATCCATCGCTCGCCAGTGCAGTTCGTCGATGCGCCGGTATTGCGCAAGCCGTCGT
GGATCCGCGTGCGGATTCCGTCCGGCAATGCCGTGCAGAACCTCAAGGCCAAGCTGCGCGAAAATCGCTTGGTCACCGTC
TGCGAAGAAGCCAGTTGCCCGAACATCCACGAGTGCTTCAGCCACGGCACCGCGACCTTCATGATCTTGGGCGAGGTGTG
CACACGGCGCTGCTCGTTCTGCGATGTGGCGCATGGCCGGCCGAAGCCGCCTGACGCAAACGAGCCGGCCAGCCTGGCCA
TCACCGTGGCCGACATGGGCCTGAAATACGTGGTGGTGACCAGCGTGGACCGCGATGATCTGCGCGACGGTGGCGCGCAA
CATTTCGTCGACTGCATCTCGGCGATCCGGACCAGTTCGCCGAATACCCGCATCGAAATTCTGACGCCGGATTTCCGTGG
CAAGGGCCGCATGGACCGCGCGCTGGACATCCTGGCGCTGAGCCCGCCGGACGTGTTCAACCACAACATCGAAACCGTGC
CGGATCTGTACCCGAACGTGCGCCCCGGCGCGGATTACCAGTGGTCGCTGACGCTGCTGCAGCGCTTCAAGGCGCAGCAT
CCGTCAATTGCTACCAAGTCCGGCATCATGCTCGGCCTGGGCGAAACGATGGAGCAGGTGCAGGCCACCTTGCGCGATCT
GCGCGCGCACGATGTGGATATGATCACCATCGGCCAGTATCTGCAGCCGACGCCGCACCATCACCCGGTGATGCGTTACT
GGACGCCGGAGGAATACAAGGCGCTGGAGGAGTACGGCAATGCGCTGGGCTTCAGTCATGTCGCCTCCGGCCCGATGGTG
CGTTCGTCGTATCACGCCGACCGCCAGGCCGCTGGCGCTGGTGTTGCTGCCTGA

Upstream 100 bases:

>100_bases
CGGTGCTGCTTGAGCAACTGGCGCGCCAGTTCGGCCTCGTGCTGCAGCCCGCTTCCGCATTGCCCGACCTTTCGCTTCCG
GCCTGAGCGCCCGTACCGTC

Downstream 100 bases:

>100_bases
GTGAGTGTCGATTCCAACGGAAATTTTTACTGCACAACGATCTATTGGACTCAGGACATCGCTACTCGCGATCAAGTGGC
GCGCAAAAGCATCCAGTTCA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTQPTARSIPLQVVSGDTAAPAPLQTGVKQIGGDKIHRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTV
CEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDANEPASLAITVADMGLKYVVVTSVDRDDLRDGGAQ
HFVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALDILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEEYGNALGFSHVASGPMV
RSSYHADRQAAGAGVAA

Sequences:

>Translated_337_residues
MTQPTARSIPLQVVSGDTAAPAPLQTGVKQIGGDKIHRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTV
CEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDANEPASLAITVADMGLKYVVVTSVDRDDLRDGGAQ
HFVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALDILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEEYGNALGFSHVASGPMV
RSSYHADRQAAGAGVAA
>Mature_336_residues
TQPTARSIPLQVVSGDTAAPAPLQTGVKQIGGDKIHRSPVQFVDAPVLRKPSWIRVRIPSGNAVQNLKAKLRENRLVTVC
EEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGRPKPPDANEPASLAITVADMGLKYVVVTSVDRDDLRDGGAQH
FVDCISAIRTSSPNTRIEILTPDFRGKGRMDRALDILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQHP
SIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYKALEEYGNALGFSHVASGPMVR
SSYHADRQAAGAGVAA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=50.1754385964912, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=48.995983935743, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=61.0591900311526, Blast_Score=417, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=46.3667820069204, Blast_Score=235, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=48.5815602836879, Blast_Score=265, Evalue=5e-72,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=49.4736842105263, Blast_Score=274, Evalue=5e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36945; Mature: 36813

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPTARSIPLQVVSGDTAAPAPLQTGVKQIGGDKIHRSPVQFVDAPVLRKPSWIRVRIP
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHCCCCHHHHCCCCCCCCCEEEEECC
SGNAVQNLKAKLRENRLVTVCEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEHHHHHHHHHCCHHHCCCCC
PKPPDANEPASLAITVADMGLKYVVVTSVDRDDLRDGGAQHFVDCISAIRTSSPNTRIEI
CCCCCCCCCCEEEEEEECCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
LTPDFRGKGRMDRALDILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
ECCCCCCCCCCCHHEEEEECCCCHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEECCCCHHHH
ALEEYGNALGFSHVASGPMVRSSYHADRQAAGAGVAA
HHHHHHHHCCHHHHCCCCCCHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
TQPTARSIPLQVVSGDTAAPAPLQTGVKQIGGDKIHRSPVQFVDAPVLRKPSWIRVRIP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHCCCCHHHHCCCCCCCCCEEEEECC
SGNAVQNLKAKLRENRLVTVCEEASCPNIHECFSHGTATFMILGEVCTRRCSFCDVAHGR
CCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCEEHHHHHHHHHCCHHHCCCCC
PKPPDANEPASLAITVADMGLKYVVVTSVDRDDLRDGGAQHFVDCISAIRTSSPNTRIEI
CCCCCCCCCCEEEEEEECCCEEEEEEEECCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEE
LTPDFRGKGRMDRALDILALSPPDVFNHNIETVPDLYPNVRPGADYQWSLTLLQRFKAQH
ECCCCCCCCCCCHHEEEEECCCCHHHCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHCC
PSIATKSGIMLGLGETMEQVQATLRDLRAHDVDMITIGQYLQPTPHHHPVMRYWTPEEYK
CCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEEECHHCCCCCCCCCEEECCCCHHHH
ALEEYGNALGFSHVASGPMVRSSYHADRQAAGAGVAA
HHHHHHHHCCHHHHCCCCCCHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]