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The map label for this gene is lipB [H]

Identifier: 84625388

GI number: 84625388

Start: 4218907

End: 4219605

Strand: Reverse

Name: lipB [H]

Synonym: XOO_3731

Alternate gene names: 84625388

Gene position: 4219605-4218907 (Counterclockwise)

Preceding gene: 84625389

Following gene: 84625387

Centisome position: 85.41

GC content: 65.09

Gene sequence:

>699_bases
GTGGACGCTGTAGCGGTCGAGCCCGTGAGGAGCTCTGCGGTCGGCCTGCCTGCGCAGTTGCGCGATCTTGGTCATCAGAG
CTACGGCCCGGTGTGGCGTGCAATGCAGCGTTTCACCGATGCGCGCGATGAGCGCACCGCCGACGAGGTCTGGGTCGTCG
AGCATGCGCCGGTGTTTACGCTGGGCCAGGCCGGCAAACCCGAGCACGTGCTGGCGCCCGGCGATATTCCGGTGCTGCAG
GTTGATCGTGGCGGGCAAGTCACGTATCACGGCCCTGGCCAATTGGTCGTTTACCCGCTGCTGGATCTGCGTCGGCTCAA
GATCGGTGTGCGCGATTACGTGTGCAAGATCGAGCAGGCACTGATCGGCATGCTGGACGAATGGAACATCGTGGCCGAGC
GTCGCGACGGTGCCCCCGGCGTTTATGTAGGTGGGGCCAAGATCGCTGCGCTCGGCATCCGCGTGCGGCGTGGCTGCACT
TTCCACGGGTTGTCGTTGAACGTGGACATGGATCTGGAGCCGTTTCACCGCATCAATCCCTGTGGCTACCAGGGCCTGCA
GGTGACCTCGGTGCTAGACTTGGGCGGCCCCTCCGGGATGGACGCCGTCAAGGCGGTGCTGCTTGAGCAACTGGCGCGCC
AGTTCGGCCTCGTGCTGCAGCCCGCTTCCGCATTGCCCGACCTTTCGCTTCCGGCCTGA

Upstream 100 bases:

>100_bases
CCAGCGGCAAATATGTCTCGGTCAAGATCGGCTTCCGCGCCGAGAGCCGCGAGCAGTTCGATGCCGCGCATCAGGCACTG
CGCGACCATCCGGAAGTGAA

Downstream 100 bases:

>100_bases
GCGCCCGTACCGTCATGACCCAGCCTACCGCCCGTTCCATCCCCTTGCAGGTCGTCTCCGGCGACACCGCCGCGCCTGCG
CCGTTGCAGACCGGCGTCAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MDAVAVEPVRSSAVGLPAQLRDLGHQSYGPVWRAMQRFTDARDERTADEVWVVEHAPVFTLGQAGKPEHVLAPGDIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIGMLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSLNVDMDLEPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLEQLARQFGLVLQPASALPDLSLPA

Sequences:

>Translated_232_residues
MDAVAVEPVRSSAVGLPAQLRDLGHQSYGPVWRAMQRFTDARDERTADEVWVVEHAPVFTLGQAGKPEHVLAPGDIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIGMLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSLNVDMDLEPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLEQLARQFGLVLQPASALPDLSLPA
>Mature_232_residues
MDAVAVEPVRSSAVGLPAQLRDLGHQSYGPVWRAMQRFTDARDERTADEVWVVEHAPVFTLGQAGKPEHVLAPGDIPVLQ
VDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQALIGMLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCT
FHGLSLNVDMDLEPFHRINPCGYQGLQVTSVLDLGGPSGMDAVKAVLLEQLARQFGLVLQPASALPDLSLPA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=141, Percent_Identity=39.7163120567376, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081767, Length=192, Percent_Identity=56.7708333333333, Blast_Score=233, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6323268, Length=127, Percent_Identity=36.2204724409449, Blast_Score=85, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21355483, Length=184, Percent_Identity=35.3260869565217, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25114; Mature: 25114

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVAVEPVRSSAVGLPAQLRDLGHQSYGPVWRAMQRFTDARDERTADEVWVVEHAPVFT
CCCCCCCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEE
LGQAGKPEHVLAPGDIPVLQVDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQA
ECCCCCCCEEECCCCCCEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
LIGMLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCTFHGLSLNVDMDLEPFHRINP
HHHHHCCCEEEEECCCCCCEEEECCCEEEEEEEEHCCCCEEECEEEEECCCCCCCCCCCC
CGYQGLQVTSVLDLGGPSGMDAVKAVLLEQLARQFGLVLQPASALPDLSLPA
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCC
>Mature Secondary Structure
MDAVAVEPVRSSAVGLPAQLRDLGHQSYGPVWRAMQRFTDARDERTADEVWVVEHAPVFT
CCCCCCCCHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEE
LGQAGKPEHVLAPGDIPVLQVDRGGQVTYHGPGQLVVYPLLDLRRLKIGVRDYVCKIEQA
ECCCCCCCEEECCCCCCEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHH
LIGMLDEWNIVAERRDGAPGVYVGGAKIAALGIRVRRGCTFHGLSLNVDMDLEPFHRINP
HHHHHCCCEEEEECCCCCCEEEECCCEEEEEEEEHCCCCEEECEEEEECCCCCCCCCCCC
CGYQGLQVTSVLDLGGPSGMDAVKAVLLEQLARQFGLVLQPASALPDLSLPA
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]