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The map label for this gene is hsdBM [H]

Identifier: 84625386

GI number: 84625386

Start: 4215823

End: 4217613

Strand: Reverse

Name: hsdBM [H]

Synonym: XOO_3729

Alternate gene names: 84625386

Gene position: 4217613-4215823 (Counterclockwise)

Preceding gene: 84625387

Following gene: 84625385

Centisome position: 85.37

GC content: 60.8

Gene sequence:

>1791_bases
ATGATGACCTTAGACGCCGCCGCAAACGTAGCTCCACTACTGACAGAGCCACTGCCTTCGCTTGCCGAACTGCCTTCACC
TTCGTATCTGCGAGCGGTTCTGCGAACACAAAGCAAGGCGACTGCTGAGCGCTCACAGCTCGCGTTTGCTGAAGCCATCA
CGGCACACGCCGTCATAGCCTATTGGGAGCAGCTTGGCTCAGGACTGGCCGTGCAGCCCCCCCCGAACGCGGCGCGCGAC
CTTCCTGAAGCTGGTCGCCGCGCCGCAAGCCAGTTTGGGCAAGCTATTGCGCGCCTGTCTGTCGAAGACGCTGCATCTAA
CCTCGGGCTACTCTACACCGCGCTCCTCCCCAGTGAGTGGCGTTCGGAGCGGGGCGTTTTTTACACCCCGCCAGCATTGG
CCGAGCGGTTGCTCGACCAAGCAGAGGCGGCAGGCCTAGATTGGTCGCGTGCCCATGTGCTCGACCCAGCAGCCGGAGCA
GGCGCGTTCCTTGTGCCTGCGGCCCGGCGACTACTGAAGTCCATCGTGGACTGTTCGCCAGCAGTAGCTATGCACAATCT
CTCTGCCAGGCTGCGCGGCTTTGAGCTTGATCCCTTTGCGGCCTGGATGGCCCAGGTGTTCGTCGAAGCAGCAGCGCTGC
CACTCATCGTGGCTTGCGGCCGGAGACCGTCCGCTGTTGTGACCGTATGCGATAGCCTCTCCATCACAAAGATAAACGGC
TTCGACTTGGTTGTCGGCAATCCGCCGTTTGGCAGGCTGAAGCTGGCCGCTGAAAGGCGCGAATACTTTTCGCGAAGCCT
CTACGGTCACGCTAACCTATACGGCCTGTTCATGGATCTGGCCGTGCGGCTGGCAAAGCCCGATGGCCTAGTGTCATTCC
TGACGCCATCGAGCTTCCTTGCCGGGGAGTACTTCAAGAATTTGCGCGCCGTCTTGCACAAGGAAGCTCCCCCGGTCTCG
CTGGACTTCGTGACGGCACGCAAGGGTGTGTTCGACGATGTGCTGCAGGAAACAGTCCTAGCCACCTATCGCAAAGGAGC
AAAGCGCGCCCGCGCTGTTGTGTCCTTCATCGAAGCGCAGCCTGGCGTGCCGGTCAAAGCTGAGCCCGCTGGCGACTTCA
CCTTACCGCGGAAAGCAACGGCCCCGTGGTTCCTGCCCCGGCACACCGATGAAGCAGAGCTTGCGAAGAGGCTGCGCGCC
ATGTCGGCGCGCCTGGCAGATTGGGGCTACAAAGTAAGCACAGGCCCCCTCGTGTGGAACCGATTCAAGCCGCAACTGTG
CGACCACGAAGAAGCTGGCACCGTGCCTCTCGTATGGGCTGAAAGCGTGACCAGCGATGGTCGTTTCGTTCCCCGAGCAG
AAAAGCGGAACCACAAGCCGTTTCTACGCCTTCAGCCTGGTAACGATTGGCTGGTCGTTCGCAAGCCCTGCGTTCTGCTC
CAGCGGACAACAGCGAAAGAGCAAGCTCGCCGGCTCATCGCTGCAGAGATGCCGGCATCGTTCATCAAGCGGCACGCCGG
CGTCACGATTGAGAACCACCTCAATATGATGATTCCGACCGTGGAAAATCCAGCGGTGTCGCCTGCGTTGCTTGCGGCGT
TTCTCAACAGCGAGGCCGCTGATCGGGCCTTCCGTTGCCTGAGCGGTAGCGTCGCGGTTTCTGCATACGAGCTTGAGAAT
TTGCCCCTCCCCACAGCAAGCGACCTGAAGAAATTGGTTGGCAGCAAAGTCACGCGCGCGAGTGTCGAGAAGGCCTGCGC
CAATCTGTATGCGACAGAAGGTGAAGCGTGA

Upstream 100 bases:

>100_bases
GCAAGCCATAGCGAAGCAGAATAAAGGTTTGGGTTGCCTGGGTTATCCGGGACGCCGCCGAGAAGTACATCGCTGCCCAA
TGGCCGCTGCCGGAGACGAA

Downstream 100 bases:

>100_bases
GCTTGCCTCCCTACGTCGACCGGCTAACCATTCACCAGCGTCTGCCCCTCATCTTTCAGGACGGCACTCCCAATCGCAAC
TATTGCATTCGGGAAATGGC

Product: methyltransferase homolog M.XphI

Products: NA

Alternate protein names: M.BsuBI; Adenine-specific methyltransferase BsuBI [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MMTLDAAANVAPLLTEPLPSLAELPSPSYLRAVLRTQSKATAERSQLAFAEAITAHAVIAYWEQLGSGLAVQPPPNAARD
LPEAGRRAASQFGQAIARLSVEDAASNLGLLYTALLPSEWRSERGVFYTPPALAERLLDQAEAAGLDWSRAHVLDPAAGA
GAFLVPAARRLLKSIVDCSPAVAMHNLSARLRGFELDPFAAWMAQVFVEAAALPLIVACGRRPSAVVTVCDSLSITKING
FDLVVGNPPFGRLKLAAERREYFSRSLYGHANLYGLFMDLAVRLAKPDGLVSFLTPSSFLAGEYFKNLRAVLHKEAPPVS
LDFVTARKGVFDDVLQETVLATYRKGAKRARAVVSFIEAQPGVPVKAEPAGDFTLPRKATAPWFLPRHTDEAELAKRLRA
MSARLADWGYKVSTGPLVWNRFKPQLCDHEEAGTVPLVWAESVTSDGRFVPRAEKRNHKPFLRLQPGNDWLVVRKPCVLL
QRTTAKEQARRLIAAEMPASFIKRHAGVTIENHLNMMIPTVENPAVSPALLAAFLNSEAADRAFRCLSGSVAVSAYELEN
LPLPTASDLKKLVGSKVTRASVEKACANLYATEGEA

Sequences:

>Translated_596_residues
MMTLDAAANVAPLLTEPLPSLAELPSPSYLRAVLRTQSKATAERSQLAFAEAITAHAVIAYWEQLGSGLAVQPPPNAARD
LPEAGRRAASQFGQAIARLSVEDAASNLGLLYTALLPSEWRSERGVFYTPPALAERLLDQAEAAGLDWSRAHVLDPAAGA
GAFLVPAARRLLKSIVDCSPAVAMHNLSARLRGFELDPFAAWMAQVFVEAAALPLIVACGRRPSAVVTVCDSLSITKING
FDLVVGNPPFGRLKLAAERREYFSRSLYGHANLYGLFMDLAVRLAKPDGLVSFLTPSSFLAGEYFKNLRAVLHKEAPPVS
LDFVTARKGVFDDVLQETVLATYRKGAKRARAVVSFIEAQPGVPVKAEPAGDFTLPRKATAPWFLPRHTDEAELAKRLRA
MSARLADWGYKVSTGPLVWNRFKPQLCDHEEAGTVPLVWAESVTSDGRFVPRAEKRNHKPFLRLQPGNDWLVVRKPCVLL
QRTTAKEQARRLIAAEMPASFIKRHAGVTIENHLNMMIPTVENPAVSPALLAAFLNSEAADRAFRCLSGSVAVSAYELEN
LPLPTASDLKKLVGSKVTRASVEKACANLYATEGEA
>Mature_596_residues
MMTLDAAANVAPLLTEPLPSLAELPSPSYLRAVLRTQSKATAERSQLAFAEAITAHAVIAYWEQLGSGLAVQPPPNAARD
LPEAGRRAASQFGQAIARLSVEDAASNLGLLYTALLPSEWRSERGVFYTPPALAERLLDQAEAAGLDWSRAHVLDPAAGA
GAFLVPAARRLLKSIVDCSPAVAMHNLSARLRGFELDPFAAWMAQVFVEAAALPLIVACGRRPSAVVTVCDSLSITKING
FDLVVGNPPFGRLKLAAERREYFSRSLYGHANLYGLFMDLAVRLAKPDGLVSFLTPSSFLAGEYFKNLRAVLHKEAPPVS
LDFVTARKGVFDDVLQETVLATYRKGAKRARAVVSFIEAQPGVPVKAEPAGDFTLPRKATAPWFLPRHTDEAELAKRLRA
MSARLADWGYKVSTGPLVWNRFKPQLCDHEEAGTVPLVWAESVTSDGRFVPRAEKRNHKPFLRLQPGNDWLVVRKPCVLL
QRTTAKEQARRLIAAEMPASFIKRHAGVTIENHLNMMIPTVENPAVSPALLAAFLNSEAADRAFRCLSGSVAVSAYELEN
LPLPTASDLKKLVGSKVTRASVEKACANLYATEGEA

Specific function: This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strand, and protects the DNA from cleavage by the BsuBI endonuclease [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 64652; Mature: 64652

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLDAAANVAPLLTEPLPSLAELPSPSYLRAVLRTQSKATAERSQLAFAEAITAHAVIA
CCCCCCCCCCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWEQLGSGLAVQPPPNAARDLPEAGRRAASQFGQAIARLSVEDAASNLGLLYTALLPSEW
HHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHH
RSERGVFYTPPALAERLLDQAEAAGLDWSRAHVLDPAAGAGAFLVPAARRLLKSIVDCSP
HCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCH
AVAMHNLSARLRGFELDPFAAWMAQVFVEAAALPLIVACGRRPSAVVTVCDSLSITKING
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
FDLVVGNPPFGRLKLAAERREYFSRSLYGHANLYGLFMDLAVRLAKPDGLVSFLTPSSFL
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHH
AGEYFKNLRAVLHKEAPPVSLDFVTARKGVFDDVLQETVLATYRKGAKRARAVVSFIEAQ
HHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGVPVKAEPAGDFTLPRKATAPWFLPRHTDEAELAKRLRAMSARLADWGYKVSTGPLVWN
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCEEEC
RFKPQLCDHEEAGTVPLVWAESVTSDGRFVPRAEKRNHKPFLRLQPGNDWLVVRKPCVLL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCCCCEEEEECCCCEEEEECCHHHH
QRTTAKEQARRLIAAEMPASFIKRHAGVTIENHLNMMIPTVENPAVSPALLAAFLNSEAA
HHHHHHHHHHHHHHHHCCHHHHHHHCCCEEECCCCEEEECCCCCCCCHHHHHHHHCCHHH
DRAFRCLSGSVAVSAYELENLPLPTASDLKKLVGSKVTRASVEKACANLYATEGEA
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMTLDAAANVAPLLTEPLPSLAELPSPSYLRAVLRTQSKATAERSQLAFAEAITAHAVIA
CCCCCCCCCCHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YWEQLGSGLAVQPPPNAARDLPEAGRRAASQFGQAIARLSVEDAASNLGLLYTALLPSEW
HHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHH
RSERGVFYTPPALAERLLDQAEAAGLDWSRAHVLDPAAGAGAFLVPAARRLLKSIVDCSP
HCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCH
AVAMHNLSARLRGFELDPFAAWMAQVFVEAAALPLIVACGRRPSAVVTVCDSLSITKING
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
FDLVVGNPPFGRLKLAAERREYFSRSLYGHANLYGLFMDLAVRLAKPDGLVSFLTPSSFL
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHH
AGEYFKNLRAVLHKEAPPVSLDFVTARKGVFDDVLQETVLATYRKGAKRARAVVSFIEAQ
HHHHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGVPVKAEPAGDFTLPRKATAPWFLPRHTDEAELAKRLRAMSARLADWGYKVSTGPLVWN
CCCCEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCEEEC
RFKPQLCDHEEAGTVPLVWAESVTSDGRFVPRAEKRNHKPFLRLQPGNDWLVVRKPCVLL
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHCCCCCCEEEEECCCCEEEEECCHHHH
QRTTAKEQARRLIAAEMPASFIKRHAGVTIENHLNMMIPTVENPAVSPALLAAFLNSEAA
HHHHHHHHHHHHHHHHCCHHHHHHHCCCEEECCCCEEEECCCCCCCCHHHHHHHHCCHHH
DRAFRCLSGSVAVSAYELENLPLPTASDLKKLVGSKVTRASVEKACANLYATEGEA
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1480472 [H]