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The map label for this gene is mpl [H]

Identifier: 84622537

GI number: 84622537

Start: 965529

End: 966887

Strand: Direct

Name: mpl [H]

Synonym: XOO_0880

Alternate gene names: 84622537

Gene position: 965529-966887 (Clockwise)

Preceding gene: 84622536

Following gene: 84622538

Centisome position: 19.54

GC content: 66.0

Gene sequence:

>1359_bases
ATGACCAAACTCCACATTCTCGGCATCGCCGGAACTTTCATGGGCGGTGTGGCCGCCCTGGCGCGTGAGCTGGGCTGGCA
GGTGGAAGGCAGCGACCAAGCCATCTATCCGCCGATGTCCACACAGCTGGAAACGCTAGGCATTGCGCTGGCGCAGGGCT
ATGCGCCGTCCAATATTTCGCCGGACGCGACCGATGTCGTCGTCGGCAACGCCTTGTCGCGCGGCAATCCGGCAGTGGAA
GCGGTGCTGGATGCCGGCCGCCGCTACACCTCGGGCGCGCAATGGTTGGCCGAGCAAGTGCTGCCTGGCCGCGACACGCT
GGCGGTCGCCGGCACTCACGGCAAGACCACCACCACCACCATCCTGAGCTACCTGTTGGAGGCTGCCGGTCGTGCGCCGG
GGTTTTTGATCGGTGGCGTGGCCGCAGACTTCGGCGTGTCGGCGCGGCTGGGCCAGGGGCGCGAATTCGTGGTGGAAGCC
GACGAGTACGACACCGCGTTTTTCGACAAGCGCAGCAAGTTCGTGCATTACCGGCCGTTGGTGGCGATCCTCAACAACCT
GGAATACGACCACGCCGATATCTTCCCGGATGTGGCCGCGATCCAGCGCCAGTTCCACCATCTGGTGCGCACGGTGCCGG
CACGCGGGCGGCTGATCGTCAATGGCGAAGACGCGCACCTGGCCGAGGTGCTGGCGATGGGCTGCTGGACGTCGGTGGAG
CAATTCGGGTTCGATGCCTCGCTGGACTGGAGCGCGCGGTTGATTGCGGCTGATGGCAGCGCCTTTGCGGTGCTGCACCG
TGGCGCGGAGGTTGGTCACGTGCAGTGGCCGTTGCTTGGGCGGCACAACGTGCTGAACGGGTTGGCGGCGCTGGCGGCGG
TACACGCAGTTGGCGTAGATCCAGCAACGGTGATGCCGGCTTTGGCGCACTTCCAGAGCGTCAAGCGGCGGCTGGAAGTC
ATTGGGAGCGCGCGCGGAATCACCGTCTACGACGACTTTGCACACCATCCCACCGCGATCGCGACCACGTTGCAGGGCTT
GCGTGCGAGAGTCGGTGCAGCGCGTGTGGTGGTGGCGATGGAGCCGCGCAGCAATTCGATGCGACTGGGCGCGCATGCGC
AGGCGTTGGCGCCGTCGCTGCACGATGCCGATGCGGTGGTGTTTCTGCATCGGCCGGAACTGGCCTGGGATGCGGCGCCG
ATCATTGCGCAAGTGCGTGGCGAGGCGCGCGTGGCACAGGACGTTGCTATCTTGTTACGCACGCTGGGTGAGATCGCGCA
GCCGGGCGACCACGTGGTGTTCATGTCCAACGGCGGCTTCGACGGTGCGCCGCGTCGTTTCCTGGCGCAGCTGTCGTGA

Upstream 100 bases:

>100_bases
ATCGGAAAATTCCCGATAGTAGGGTAGGGTTTTCCTGACGCCTGGGCTTGCGTTGGATTGAAATGTCCCGCAACTCCGGC
GATCGGCGACAATGGACGGC

Downstream 100 bases:

>100_bases
TGGCGCCGCTCATGCACGCCGCCGAGACCAGCGCCTTGCCGCTGTTTCCGTTGCACAACGTGTTGCTGCCAGGCGCGGCA
ATGGGGTTGCGTGTGTTCGA

Product: UDP-N-acetylmuramate-L-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNPAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAADFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTSVE
QFGFDASLDWSARLIAADGSAFAVLHRGAEVGHVQWPLLGRHNVLNGLAALAAVHAVGVDPATVMPALAHFQSVKRRLEV
IGSARGITVYDDFAHHPTAIATTLQGLRARVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVAQDVAILLRTLGEIAQPGDHVVFMSNGGFDGAPRRFLAQLS

Sequences:

>Translated_452_residues
MTKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNPAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAADFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTSVE
QFGFDASLDWSARLIAADGSAFAVLHRGAEVGHVQWPLLGRHNVLNGLAALAAVHAVGVDPATVMPALAHFQSVKRRLEV
IGSARGITVYDDFAHHPTAIATTLQGLRARVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVAQDVAILLRTLGEIAQPGDHVVFMSNGGFDGAPRRFLAQLS
>Mature_451_residues
TKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNPAVEA
VLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAADFGVSARLGQGREFVVEAD
EYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTSVEQ
FGFDASLDWSARLIAADGSAFAVLHRGAEVGHVQWPLLGRHNVLNGLAALAAVHAVGVDPATVMPALAHFQSVKRRLEVI
GSARGITVYDDFAHHPTAIATTLQGLRARVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPI
IAQVRGEARVAQDVAILLRTLGEIAQPGDHVVFMSNGGFDGAPRRFLAQLS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=52.9933481152993, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786279, Length=354, Percent_Identity=34.180790960452, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48069; Mature: 47938

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CCEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
PDATDVVVGNALSRGNPAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCCHHEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAADFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTSVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
QFGFDASLDWSARLIAADGSAFAVLHRGAEVGHVQWPLLGRHNVLNGLAALAAVHAVGVD
HCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAHFQSVKRRLEVIGSARGITVYDDFAHHPTAIATTLQGLRARVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVAQDVAILLR
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
TLGEIAQPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CEEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
PDATDVVVGNALSRGNPAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCCHHEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAADFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECHHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTSVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHH
QFGFDASLDWSARLIAADGSAFAVLHRGAEVGHVQWPLLGRHNVLNGLAALAAVHAVGVD
HCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAHFQSVKRRLEVIGSARGITVYDDFAHHPTAIATTLQGLRARVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVAQDVAILLR
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHH
TLGEIAQPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]