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The map label for this gene is leuD [H]

Identifier: 84622515

GI number: 84622515

Start: 935639

End: 936286

Strand: Direct

Name: leuD [H]

Synonym: XOO_0858

Alternate gene names: 84622515

Gene position: 935639-936286 (Clockwise)

Preceding gene: 84622514

Following gene: 84622516

Centisome position: 18.94

GC content: 60.8

Gene sequence:

>648_bases
ATGACTCCTTTCACCCAACACACCGGACTGGTCGCGCCTCTGGATCGCGCCAACGTCGATACCGACCAGATCATTCCCAA
GCAGTTTCTCAAGTCGATCAAGCGCACCGGCTTCGGGCCGAATCTGTTCGACGAGTGGCGCTATCTGGATATCGGCGAAC
CTGGTCGCGACAACAGCACGCGCCCGCTGAATCCGGAATTCGTGCTCAATTTCCCGCGATATCAGGGCGCCAGCGTGTTG
TTGGCACGCGAAAACTTCGGCTGCGGTTCCTCGCGCGAGCATGCGCCGTGGGCGCTGGACGAATACGGCTTTCGCGCCGT
CATCGCGCCGAGCTTTGCCGATATTTTTTACAACAACAGCTTCAAGAACGGCCTGCTGCCGATCGTGCTGGCCGAAGCGG
AAGTGGATGCGCTGTTCGAACAGTGCCTGGCCAACGAGGGCTATCAACTCACCGTGGATCTGGCCGCACAGCGCGTGCGC
CGCCCCGATGGCGTGGAATACAGTTTCGACATCGACGCATTCCGCAAGCACTGCCTGCTCAACGGCCTGGACGACATCGG
CCTGACCTTGCAGGAGGCCGATGCCATCGGTCGCTTCGAGCAGGACCATCGCGCACGCCAACCGTGGTTGTTTGGCGCAC
TGCAGTGA

Upstream 100 bases:

>100_bases
GGGGATTCGGGAGTGGGGATTCGGGATTCGTAAAGCCGGTCATGCACTTACGCCTTTTTCGAACCCCCAATCCCTCAACC
CCGAATCCCGGCCTCTCTCA

Downstream 100 bases:

>100_bases
ATTCGGTCGCCAAAACCACTGATCCGTCGGCGCTGAGCGAACACGTCGCTGCCCAGTTCGGGCCGCAGGCGCACGCCTAT
CTGCACAGCGATGTGCATGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTPFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVL
LARENFGCGSSREHAPWALDEYGFRAVIAPSFADIFYNNSFKNGLLPIVLAEAEVDALFEQCLANEGYQLTVDLAAQRVR
RPDGVEYSFDIDAFRKHCLLNGLDDIGLTLQEADAIGRFEQDHRARQPWLFGALQ

Sequences:

>Translated_215_residues
MTPFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVL
LARENFGCGSSREHAPWALDEYGFRAVIAPSFADIFYNNSFKNGLLPIVLAEAEVDALFEQCLANEGYQLTVDLAAQRVR
RPDGVEYSFDIDAFRKHCLLNGLDDIGLTLQEADAIGRFEQDHRARQPWLFGALQ
>Mature_214_residues
TPFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNSTRPLNPEFVLNFPRYQGASVLL
ARENFGCGSSREHAPWALDEYGFRAVIAPSFADIFYNNSFKNGLLPIVLAEAEVDALFEQCLANEGYQLTVDLAAQRVRR
PDGVEYSFDIDAFRKHCLLNGLDDIGLTLQEADAIGRFEQDHRARQPWLFGALQ

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=205, Percent_Identity=64.390243902439, Blast_Score=260, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6321429, Length=218, Percent_Identity=51.3761467889908, Blast_Score=211, Evalue=6e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 24281; Mature: 24149

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNST
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPEFVLNFPRYQGASVLLARENFGCGSSREHAPWALDEYGFRAVIAPSFADIFYNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCEEEECCHHHHHHCCCC
FKNGLLPIVLAEAEVDALFEQCLANEGYQLTVDLAAQRVRRPDGVEYSFDIDAFRKHCLL
CCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECHHHHHHHHHH
NGLDDIGLTLQEADAIGRFEQDHRARQPWLFGALQ
CCCHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
TPFTQHTGLVAPLDRANVDTDQIIPKQFLKSIKRTGFGPNLFDEWRYLDIGEPGRDNST
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCEEEECCCCCCCCCC
RPLNPEFVLNFPRYQGASVLLARENFGCGSSREHAPWALDEYGFRAVIAPSFADIFYNNS
CCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHCCCCEEEECCHHHHHHCCCC
FKNGLLPIVLAEAEVDALFEQCLANEGYQLTVDLAAQRVRRPDGVEYSFDIDAFRKHCLL
CCCCCEEEEEEHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCEEEECHHHHHHHHHH
NGLDDIGLTLQEADAIGRFEQDHRARQPWLFGALQ
CCCHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]