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The map label for this gene is leuC [H]

Identifier: 84622514

GI number: 84622514

Start: 934123

End: 935571

Strand: Direct

Name: leuC [H]

Synonym: XOO_0857

Alternate gene names: 84622514

Gene position: 934123-935571 (Clockwise)

Preceding gene: 84622512

Following gene: 84622515

Centisome position: 18.91

GC content: 64.73

Gene sequence:

>1449_bases
ATGACTGCCAAGACCCTGTACGACAAGCTGTGGGAAATGCACGAGGTCACACGCCGTGACGATGGTTCGTCGCTGATCTA
CATCGACCGCCACATCCTGCATGAAGTGACTTCGCCGCAAGCCTTCGAAGGCCTGCGCCTGGCCGGGCGCAATCCGTGGC
GCATCGACGCCAACATCGCCACGCCCGATCACAACGTGCCGACCACACGCGCCGAGCGCCAGGGCGGGCTGGAGTCGATT
TCCGACGAGGTGTCGCGCCTGCAGGTGCAGACGCTGGACGAGAATTGCGACGATTTCGGCATCCTCGAGTTCAAGATGAA
CGATGCACGCCAGGGCATCGTGCATGTGGTCGGCCCGGAGCAGGGCGCCACCTTGCCGGGCATGACCGTGGTCTGCGGCG
ACTCGCACACCTCCACGCACGGCGCATTCGGCGCGTTGGCGCATGGCATCGGCACCTCCGAGGTCGAGCATGTGCTGGCC
ACCCAGTGCCTGATCACCAAGAAGATGAAAAACCTGCAGGTGCGCGTGGAAGGCACGCTGCCGTTCGGGGTGACCGCCAA
GGACATCGTGCTGGCGGTGATCGGCAAGATCGGCACCGCCGGCGGCAACGGGCATGCGCTGGAATTTGCCGGCAGCGCCA
TCCGCGCGCTGTCGATGGAAGGCCGCATGACCATCTGCAATATGTCCATCGAAGCGGGTGCACGCGTGGGCATGGTGGCG
GTGGACGAGAAGACCATCGCCTACGTCAAAGGCCGCCCGTTCGCACCCAAGGGCGCCGACTGGGACGCGGCGGTCGCGCT
GTGGAGCACCTTGGTGTCCGACCCGGACGCACACTTCGACACCGTGGTGGAACTGCATGCCGAAGACATCAAGCCGCAGG
TCAGCTGGGGCACGTCGCCGGAGATGGTGCTGGCCATCGACCAGCACGTACCGGATCCGGCGACCGAGCAGGATCCGACC
AAGCGCAATTCGATCGAGCGCGCGTTGAAATACATGGGTTTGAAGGCCAACCAGGCGATCACCGACATCCGCCTGGATCG
CGTGTTCATTGGCTCGTGCACCAACTCGCGGATCGAAGACCTGCGCGCGGCCGCCGCAGTGGCCAAGGGACGCAAGGTCG
CGTCAACCATCAAGCAGGCGCTGGTGGTGCCGGGTTCTGGCTTGGTCAAGGCGCAGGCAGAGGCGGAAGGCCTGGACAAG
GTGTTCCTGGATGCCGGTTTCGAATGGCGCGAGCCGGGCTGCTCGATGTGTCTGGCCATGAATCCGGACAAGCTCGGCAG
CGGCGAGCACTGCGCTTCCACCTCCAACCGCAACTTCGAAGGCCGCCAGGGCGCCGGTGGCCGCACGCATTTGGTCAGCC
CGGCGATGGCCGCTGCAGCAGCGGTGAGCGGGCATTTTGTCGATGTGCGTGAACTCGGGGATTCGGGAGTGGGGATTCGG
GATTCGTAA

Upstream 100 bases:

>100_bases
TCTTGAATAAACAATATGAATTTGCGTAATCCAAGACAAGCTCCTAGCATCGGTCTCAGCCAGGTGGGCGCACCCACCAT
CCGCCACTCGGATTGCCGCG

Downstream 100 bases:

>100_bases
AGCCGGTCATGCACTTACGCCTTTTTCGAACCCCCAATCCCTCAACCCCGAATCCCGGCCTCTCTCAATGACTCCTTTCA
CCCAACACACCGGACTGGTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTAKTLYDKLWEMHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRNPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLITKKMKNLQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRALSMEGRMTICNMSIEAGARVGMVA
VDEKTIAYVKGRPFAPKGADWDAAVALWSTLVSDPDAHFDTVVELHAEDIKPQVSWGTSPEMVLAIDQHVPDPATEQDPT
KRNSIERALKYMGLKANQAITDIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
DS

Sequences:

>Translated_482_residues
MTAKTLYDKLWEMHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRNPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLITKKMKNLQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRALSMEGRMTICNMSIEAGARVGMVA
VDEKTIAYVKGRPFAPKGADWDAAVALWSTLVSDPDAHFDTVVELHAEDIKPQVSWGTSPEMVLAIDQHVPDPATEQDPT
KRNSIERALKYMGLKANQAITDIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
DS
>Mature_481_residues
TAKTLYDKLWEMHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRNPWRIDANIATPDHNVPTTRAERQGGLESIS
DEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLITKKMKNLQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRALSMEGRMTICNMSIEAGARVGMVAV
DEKTIAYVKGRPFAPKGADWDAAVALWSTLVSDPDAHFDTVVELHAEDIKPQVSWGTSPEMVLAIDQHVPDPATEQDPTK
RNSIERALKYMGLKANQAITDIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKV
FLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIRD
S

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=29.2011019283747, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=26.7015706806283, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=63.6170212765957, Blast_Score=600, Evalue=1e-173,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=25.2747252747253, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081781, Length=363, Percent_Identity=24.5179063360882, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=373, Percent_Identity=28.4182305630027, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=375, Percent_Identity=28.8, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=315, Percent_Identity=28.5714285714286, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=27.1334792122538, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=62.5531914893617, Blast_Score=600, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=28.7292817679558, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6320440, Length=357, Percent_Identity=27.4509803921569, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322261, Length=481, Percent_Identity=26.1954261954262, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI281365315, Length=398, Percent_Identity=27.6381909547739, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17864292, Length=398, Percent_Identity=27.6381909547739, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161076999, Length=398, Percent_Identity=27.6381909547739, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28571643, Length=375, Percent_Identity=26.6666666666667, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.5590551181102, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI17137564, Length=380, Percent_Identity=27.1052631578947, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51561; Mature: 51430

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTLYDKLWEMHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRNPWRIDANIA
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHCCCCCEEEEEEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLITKKMKNLQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRALSMEGRMTICNMSIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHEEECCCCEEEEEEECCCCCEEEEEE
VDEKTIAYVKGRPFAPKGADWDAAVALWSTLVSDPDAHFDTVVELHAEDIKPQVSWGTSP
ECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPATEQDPTKRNSIERALKYMGLKANQAITDIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHEECCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHCCCCCCCCC
DS
CC
>Mature Secondary Structure 
TAKTLYDKLWEMHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRNPWRIDANIA
CHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCHHCCHHHCCCCCEEEEEEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDARQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLITKKMKNLQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRALSMEGRMTICNMSIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHEEECCCCEEEEEEECCCCCEEEEEE
VDEKTIAYVKGRPFAPKGADWDAAVALWSTLVSDPDAHFDTVVELHAEDIKPQVSWGTSP
ECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPATEQDPTKRNSIERALKYMGLKANQAITDIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHEECCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHCCCCCCCCC
DS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA