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The map label for this gene is rihA [H]

Identifier: 84622482

GI number: 84622482

Start: 896767

End: 897765

Strand: Direct

Name: rihA [H]

Synonym: XOO_0825

Alternate gene names: 84622482

Gene position: 896767-897765 (Clockwise)

Preceding gene: 84622481

Following gene: 84622483

Centisome position: 18.15

GC content: 67.27

Gene sequence:

>999_bases
ATGCAGCGGACCTGGCAACGCGCGTGGGGATTGGTGGCAATGTGGTGCATGTCGACGGCCGTGTGGGCGCAGCAGCCTGT
AGCGGCCGTGCAATCGACACCTGCATTGGTGCAGACCGAGAAGGTGATGGTGTCCACCGACATCGGCGACGACATCGACG
ATGCGTTCGCGTTGGGGCTGCTGCTGCGTAGCCCGCAGTTGCAGGTGCTGGGCATCGCATCGGCGTGGGGCGACACCACG
TTGCGTGCGCAGTTGCTGCAGCGGTTATTGCAGCAGGTCGGGCGCAGCGAGATCCCGCTGGCGGTGGGGGCGCGCACCAC
CAGCAGTATTGCTTTCAGCCAGGCGCGCTGGGCGGCCAGGGGGCAGTTGCCGGCCGCGCTGCCGGATGCGGCCACGATGA
TCCTGCAGCAGGCGCGCCAGCATCCGGGCGAGGTCACCTTGCTGGTGCTGGGGCCGATGACCGATGCCGCGCTGGCGCAA
CAACGCGACCCGGTCGGGTTTGCCAAGCTCAAGCGTGTGGTGGCGATGGGCGGGTCGGTGCGGGTGGGTTACGGCACATC
TGCGTATCGCCCGGCCAGTGCGCCGGCGCCGGAGTACAACATCCTGGCCGACGTGCCGGCAGCGCAACGCGTGTTTGCGG
CCGGGGTGCCGATCGTGTTGTTGCCACTGGATGCCACGCAGATCACGCTGGAAGAGCCCGAGCGCATCGCGCTGTTTGCG
CATGGCGACGGGCTGACCGATGCGCTGACTCAGCTGTACTACCAATGGCGCAACACCGACCAGCCCTGGGCCAGTGCCAC
GCCCACGGTGTTCGATGCGGTGCCGGTGGCCTGGTTGCTGCGACCGGCGCTGTGCCCGACCACGCCGCTGCATCTGGACG
TTGATGCGCAGGGCTATACCCGCGAAGGGGCTGGCAAACCGAATGTGCAGGCCTGCCTGCGCGTGGACAAGCCGGCGCTG
ATCGAGATGTACATGCGTGCGATGTTGGAGTTGCACTGA

Upstream 100 bases:

>100_bases
GCAGTTGCGCGCCAACTTCTGATCGGCGCCGTGGTGTAGCAACATCCAACCGCCGGCGATCATGCCGGCGGTTTTTTTTT
ATGGCAGCAGGAGTGGGCAG

Downstream 100 bases:

>100_bases
AAGTGGCGAATAAAACTTGGTGAATCCCGTGGAGTGCGGTGAGTGCAGTTTTTTCGCGGCATCGTTTCAGCTGTAGTCAA
AAGCCTGGTTGGTAGCGCGC

Product: inosine-uridine preferring nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQRTWQRAWGLVAMWCMSTAVWAQQPVAAVQSTPALVQTEKVMVSTDIGDDIDDAFALGLLLRSPQLQVLGIASAWGDTT
LRAQLLQRLLQQVGRSEIPLAVGARTTSSIAFSQARWAARGQLPAALPDAATMILQQARQHPGEVTLLVLGPMTDAALAQ
QRDPVGFAKLKRVVAMGGSVRVGYGTSAYRPASAPAPEYNILADVPAAQRVFAAGVPIVLLPLDATQITLEEPERIALFA
HGDGLTDALTQLYYQWRNTDQPWASATPTVFDAVPVAWLLRPALCPTTPLHLDVDAQGYTREGAGKPNVQACLRVDKPAL
IEMYMRAMLELH

Sequences:

>Translated_332_residues
MQRTWQRAWGLVAMWCMSTAVWAQQPVAAVQSTPALVQTEKVMVSTDIGDDIDDAFALGLLLRSPQLQVLGIASAWGDTT
LRAQLLQRLLQQVGRSEIPLAVGARTTSSIAFSQARWAARGQLPAALPDAATMILQQARQHPGEVTLLVLGPMTDAALAQ
QRDPVGFAKLKRVVAMGGSVRVGYGTSAYRPASAPAPEYNILADVPAAQRVFAAGVPIVLLPLDATQITLEEPERIALFA
HGDGLTDALTQLYYQWRNTDQPWASATPTVFDAVPVAWLLRPALCPTTPLHLDVDAQGYTREGAGKPNVQACLRVDKPAL
IEMYMRAMLELH
>Mature_332_residues
MQRTWQRAWGLVAMWCMSTAVWAQQPVAAVQSTPALVQTEKVMVSTDIGDDIDDAFALGLLLRSPQLQVLGIASAWGDTT
LRAQLLQRLLQQVGRSEIPLAVGARTTSSIAFSQARWAARGQLPAALPDAATMILQQARQHPGEVTLLVLGPMTDAALAQ
QRDPVGFAKLKRVVAMGGSVRVGYGTSAYRPASAPAPEYNILADVPAAQRVFAAGVPIVLLPLDATQITLEEPERIALFA
HGDGLTDALTQLYYQWRNTDQPWASATPTVFDAVPVAWLLRPALCPTTPLHLDVDAQGYTREGAGKPNVQACLRVDKPAL
IEMYMRAMLELH

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=299, Percent_Identity=29.0969899665552, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788486, Length=324, Percent_Identity=26.5432098765432, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786213, Length=274, Percent_Identity=26.6423357664234, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 35906; Mature: 35906

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTWQRAWGLVAMWCMSTAVWAQQPVAAVQSTPALVQTEKVMVSTDIGDDIDDAFALGL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEECEEEEEECCCCCHHHHHHHHH
LLRSPQLQVLGIASAWGDTTLRAQLLQRLLQQVGRSEIPLAVGARTTSSIAFSQARWAAR
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHC
GQLPAALPDAATMILQQARQHPGEVTLLVLGPMTDAALAQQRDPVGFAKLKRVVAMGGSV
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCCE
RVGYGTSAYRPASAPAPEYNILADVPAAQRVFAAGVPIVLLPLDATQITLEEPERIALFA
EECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEEE
HGDGLTDALTQLYYQWRNTDQPWASATPTVFDAVPVAWLLRPALCPTTPLHLDVDAQGYT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
REGAGKPNVQACLRVDKPALIEMYMRAMLELH
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQRTWQRAWGLVAMWCMSTAVWAQQPVAAVQSTPALVQTEKVMVSTDIGDDIDDAFALGL
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEECEEEEEECCCCCHHHHHHHHH
LLRSPQLQVLGIASAWGDTTLRAQLLQRLLQQVGRSEIPLAVGARTTSSIAFSQARWAAR
HHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHC
GQLPAALPDAATMILQQARQHPGEVTLLVLGPMTDAALAQQRDPVGFAKLKRVVAMGGSV
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCCE
RVGYGTSAYRPASAPAPEYNILADVPAAQRVFAAGVPIVLLPLDATQITLEEPERIALFA
EECCCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEEE
HGDGLTDALTQLYYQWRNTDQPWASATPTVFDAVPVAWLLRPALCPTTPLHLDVDAQGYT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
REGAGKPNVQACLRVDKPALIEMYMRAMLELH
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA