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The map label for this gene is lpsJ [H]

Identifier: 84622384

GI number: 84622384

Start: 783273

End: 783902

Strand: Direct

Name: lpsJ [H]

Synonym: XOO_0727

Alternate gene names: 84622384

Gene position: 783273-783902 (Clockwise)

Preceding gene: 84622383

Following gene: 84622387

Centisome position: 15.86

GC content: 62.7

Gene sequence:

>630_bases
ATGGCCTGGACCAGAGACCACATGGCCGCACGCGCGGCACGCGAACTGACCGATGGCGCTTACGTCAACCTGGGTATCGG
CTTGCCGACGTTGGTTGCCAATCACATCCCCGACGGTGTGGATGTCTGGCTGCAATCGGAGAACGGCCTGCTCGGCATTG
GCCCGTTTCCCACCGAAGACGAGGTGGACGCGGACCTGATCAATGCGGGCAAGCAGACCGTGACCGCGCGTGCCGGTGCC
AGTTATTTCGGTAGCCACGATTCGTTCGCGATGATCCGTGGCGGGCACATCGACCTGGCGATTTTGGGCGCAATGCAGGT
CACCGAGCGCGGCGACCTGGCCAACTGGATGGTGCCCGGCAAAATGGTCAAGGGCATGGGCGGTGCGATGGACCTGGTGG
CCGGCGTCAAGCGCGTGGTGGTGTTGATGGAGCACGTCGCCAAAGACGGCAGCCACAAGATCCTGCGCGACTGCGACCTG
CCACTGACCGGCGTCGGCGTGGTGGATCGCATCATTACCGATCTCGCCGTGTTCGATGTCACTGCGGATGGGCTGGTACT
GGTGGAATCTGCAGAGGGTGTGGAGCTGGAAGAGTTGCGCCAGAAGACTGGGGTTGAATTCGCTGCGTGA

Upstream 100 bases:

>100_bases
ATCAGGTGCATCTGCCCGGTATTTACGTGGACCGCCTGGTGCTCAATGCCACGCCGGAAAAACGCATCGAACAGCGCACC
GTGCGCGAAGGACAGCAGTA

Downstream 100 bases:

>100_bases
TCGAAAGCTATCTACGCAGCGACTAGCTCATGAGCAGCTAGGGACATGCATGGCGCCTCAGGGCGCCTTGCGTGCATAGC
GGTAAGGTTCGGCTGTTGCA

Product: IpsJ protein

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MAWTRDHMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAGA
SYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMEHVAKDGSHKILRDCDL
PLTGVGVVDRIITDLAVFDVTADGLVLVESAEGVELEELRQKTGVEFAA

Sequences:

>Translated_209_residues
MAWTRDHMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAGA
SYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMEHVAKDGSHKILRDCDL
PLTGVGVVDRIITDLAVFDVTADGLVLVESAEGVELEELRQKTGVEFAA
>Mature_208_residues
AWTRDHMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTEDEVDADLINAGKQTVTARAGAS
YFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPGKMVKGMGGAMDLVAGVKRVVVLMEHVAKDGSHKILRDCDLP
LTGVGVVDRIITDLAVFDVTADGLVLVESAEGVELEELRQKTGVEFAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=206, Percent_Identity=60.1941747572816, Blast_Score=252, Evalue=2e-67,
Organism=Homo sapiens, GI11545841, Length=206, Percent_Identity=60.6796116504854, Blast_Score=246, Evalue=1e-65,
Organism=Escherichia coli, GI1788552, Length=203, Percent_Identity=53.6945812807882, Blast_Score=214, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17531517, Length=206, Percent_Identity=58.252427184466, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21358325, Length=205, Percent_Identity=61.9512195121951, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24655794, Length=205, Percent_Identity=61.9512195121951, Blast_Score=260, Evalue=4e-70,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 22146; Mature: 22014

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTRDHMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTED
CCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCC
EVDADLINAGKQTVTARAGASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPG
CCCHHHHHCCCCEEEHHCCCHHCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCCCH
KMVKGMGGAMDLVAGVKRVVVLMEHVAKDGSHKILRDCDLPLTGVGVVDRIITDLAVFDV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHEEE
TADGLVLVESAEGVELEELRQKTGVEFAA
CCCCEEEEECCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
AWTRDHMAARAARELTDGAYVNLGIGLPTLVANHIPDGVDVWLQSENGLLGIGPFPTED
CCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCC
EVDADLINAGKQTVTARAGASYFGSHDSFAMIRGGHIDLAILGAMQVTERGDLANWMVPG
CCCHHHHHCCCCEEEHHCCCHHCCCCCCEEEEECCCEEEEEECHHHHCCCCCCCCCCCCH
KMVKGMGGAMDLVAGVKRVVVLMEHVAKDGSHKILRDCDLPLTGVGVVDRIITDLAVFDV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHEEE
TADGLVLVESAEGVELEELRQKTGVEFAA
CCCCEEEEECCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]