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The map label for this gene is fmt [H]

Identifier: 84622204

GI number: 84622204

Start: 597503

End: 598426

Strand: Direct

Name: fmt [H]

Synonym: XOO_0547

Alternate gene names: 84622204

Gene position: 597503-598426 (Clockwise)

Preceding gene: 84622203

Following gene: 84622205

Centisome position: 12.09

GC content: 70.02

Gene sequence:

>924_bases
ATGAGAATCATCTTTGCCGGTACGCCGGATTTCGCTGTCGCTTCGTTGCGCGCTGCCGCGCAGCGGCATGAGGTGGTGGC
GGTCTACACCCAGCCGGATCGGCCCGCCGGGCGCGGCCGCGGGTTGACGCCCTCGCCGGTCAAGATCGAGGCGATTGCGC
GCGGCATTGCGGTGTTCCAGCCGCAGACGCTGCGTTCGCCCGAAGCGCTGGCGACGCTACGTTCGCTCAATGCGGACCTG
ATGGTGGTGGTGGCCTACGGATTAATTCTGCCCAATGCGGTGCTGGCTGTGCCAACCCATGGCTGCTGGAACGTGCATGC
CTCGCTGCTGCCGCGCTGGCGTGGCGCGGCGCCAATCCAGCGCGCGATCGAGGCCGGCGATACCGAGACCGGGGTGTGCC
TGATGCAGATGGAAGCCGGGTTGGATATCGGCCCGGTGCTGCTGTCGCAGCGCATCGAGATCGGCGAGCAGGAAACCGGC
GGCCAGCTGCACGACCGCCTGGCTGCGTTGGGGGCGCAGGTATTGTCCGACGGCCTGGGCCTGCTGCGCGCCGGCATCCG
TCCGGTGGCGCAACCGCAGCCGGCTGAAGGCGTGACCTATGCGCATAAGCTGGACAAGGCGCAGGCGCGGCTGGATTGGG
CGCAGCCGGCGCAGGAACTGGCGCGGCGCGTGCGGGCGTTCAACCCGTGGCCGGTGGCCGAGGCGATCCTGGCTGGCGAG
CGCGTGCGACTGCATGGCGCAGTCGCGTTGGAGCTGGCGCATCAGCAGCCGCCCGGCAGCCTGCTGGCGGCCAGCAAGCA
GGGCATCGACATCGCGTGCGGTCAGGGTGCGCTGCGGGTGCGGGTGCTCCAGCGCGAGGGGGGCAAGGCGATCACCGCCG
CCGATTATCTCAACGCGCGGCGCGACCTGCTGGCGCTGCGCTGA

Upstream 100 bases:

>100_bases
CGCGAATCCCGCTTCCACCTCTCAGCTCTACATTACTCTCGCCCCGGTCAACACCCCCGCCGTTGCGACTCCCCACTCAC
CACTCCCGAATCCCAGCTCT

Downstream 100 bases:

>100_bases
TGTCGGCCGAGACCTCAACCGCCGCCGGCATCGCCTCGCGCCTGGCCGCCGCACGCGTTCTGACCGCCGTGTTCGATCAG
GGCCGCTCGCTGAAAGCCGA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRIIFAGTPDFAVASLRAAAQRHEVVAVYTQPDRPAGRGRGLTPSPVKIEAIARGIAVFQPQTLRSPEALATLRSLNADL
MVVVAYGLILPNAVLAVPTHGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDIGPVLLSQRIEIGEQETG
GQLHDRLAALGAQVLSDGLGLLRAGIRPVAQPQPAEGVTYAHKLDKAQARLDWAQPAQELARRVRAFNPWPVAEAILAGE
RVRLHGAVALELAHQQPPGSLLAASKQGIDIACGQGALRVRVLQREGGKAITAADYLNARRDLLALR

Sequences:

>Translated_307_residues
MRIIFAGTPDFAVASLRAAAQRHEVVAVYTQPDRPAGRGRGLTPSPVKIEAIARGIAVFQPQTLRSPEALATLRSLNADL
MVVVAYGLILPNAVLAVPTHGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDIGPVLLSQRIEIGEQETG
GQLHDRLAALGAQVLSDGLGLLRAGIRPVAQPQPAEGVTYAHKLDKAQARLDWAQPAQELARRVRAFNPWPVAEAILAGE
RVRLHGAVALELAHQQPPGSLLAASKQGIDIACGQGALRVRVLQREGGKAITAADYLNARRDLLALR
>Mature_307_residues
MRIIFAGTPDFAVASLRAAAQRHEVVAVYTQPDRPAGRGRGLTPSPVKIEAIARGIAVFQPQTLRSPEALATLRSLNADL
MVVVAYGLILPNAVLAVPTHGCWNVHASLLPRWRGAAPIQRAIEAGDTETGVCLMQMEAGLDIGPVLLSQRIEIGEQETG
GQLHDRLAALGAQVLSDGLGLLRAGIRPVAQPQPAEGVTYAHKLDKAQARLDWAQPAQELARRVRAFNPWPVAEAILAGE
RVRLHGAVALELAHQQPPGSLLAASKQGIDIACGQGALRVRVLQREGGKAITAADYLNARRDLLALR

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=292, Percent_Identity=30.4794520547945, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI21614513, Length=296, Percent_Identity=27.7027027027027, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI164663775, Length=314, Percent_Identity=28.9808917197452, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789683, Length=306, Percent_Identity=51.6339869281046, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788589, Length=272, Percent_Identity=29.0441176470588, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI133930964, Length=321, Percent_Identity=26.4797507788162, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319458, Length=276, Percent_Identity=25.3623188405797, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=31.7152103559871, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=35.5263157894737, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24585660, Length=261, Percent_Identity=27.9693486590038, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 32715; Mature: 32715

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFAGTPDFAVASLRAAAQRHEVVAVYTQPDRPAGRGRGLTPSPVKIEAIARGIAVFQ
CEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
PQTLRSPEALATLRSLNADLMVVVAYGLILPNAVLAVPTHGCWNVHASLLPRWRGAAPIQ
CHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCCCCCHHH
RAIEAGDTETGVCLMQMEAGLDIGPVLLSQRIEIGEQETGGQLHDRLAALGAQVLSDGLG
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLRAGIRPVAQPQPAEGVTYAHKLDKAQARLDWAQPAQELARRVRAFNPWPVAEAILAGE
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCC
RVRLHGAVALELAHQQPPGSLLAASKQGIDIACGQGALRVRVLQREGGKAITAADYLNAR
EEEEEHHEEEEECCCCCCCCEECCCCCCCEEEECCCCEEEEEEECCCCCEEEHHHHHHHH
RDLLALR
HHHHCCC
>Mature Secondary Structure
MRIIFAGTPDFAVASLRAAAQRHEVVAVYTQPDRPAGRGRGLTPSPVKIEAIARGIAVFQ
CEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHCC
PQTLRSPEALATLRSLNADLMVVVAYGLILPNAVLAVPTHGCWNVHASLLPRWRGAAPIQ
CHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCCCCCCCHHH
RAIEAGDTETGVCLMQMEAGLDIGPVLLSQRIEIGEQETGGQLHDRLAALGAQVLSDGLG
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LLRAGIRPVAQPQPAEGVTYAHKLDKAQARLDWAQPAQELARRVRAFNPWPVAEAILAGE
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHCCC
RVRLHGAVALELAHQQPPGSLLAASKQGIDIACGQGALRVRVLQREGGKAITAADYLNAR
EEEEEHHEEEEECCCCCCCCEECCCCCCCEEEECCCCEEEEEEECCCCCEEEHHHHHHHH
RDLLALR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]