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The map label for this gene is rsmB [H]

Identifier: 84622205

GI number: 84622205

Start: 598426

End: 599739

Strand: Direct

Name: rsmB [H]

Synonym: XOO_0548

Alternate gene names: 84622205

Gene position: 598426-599739 (Clockwise)

Preceding gene: 84622204

Following gene: 84622206

Centisome position: 12.11

GC content: 68.95

Gene sequence:

>1314_bases
ATGTCGGCCGAGACCTCAACCGCCGCCGGCATCGCCTCGCGCCTGGCCGCCGCACGCGTTCTGACCGCCGTGTTCGATCA
GGGCCGCTCGCTGAAAGCCGAACTGGCTGCGACCTTGCCCGGTATCGGCGATCCGCGCGACCGCGCACTGGTGGAGGCGA
TCTGCTTTGCGGTGATCCGCCGTCGCCCGGCGTATGACGTCGCGCTGCGGCAATGGCTGGAACGGCCGCTGCCGCCCCGC
GATGCAGAACTCAAGGCGCTGCTGATGGTCGGCTTCGCCCAGCTCGACGTGCTACAACTTCCCGCGCACGCGGCGTTGTC
GGCCACCGTGGATGCATGCCGCGCGCTGGGCCGGCCACGTCAGGCCGGCATGGTCAACGCGATCTTGCGACGCGCCCAGC
GCGAAGGGTTTCCGGCGGTATCGGATGATGCCGGCTGGCCGTCGTGGCTGCGCAAGCAGGTGCGCGCCGATTGGGGCGAG
CGTGCAGAAGGCATTTTTATCGCGAGTGCGCAGATGGCGCCGATGTGGCTGCGTGTGCAGCATTCGCGGATCGACCCGGC
GGCCTACGTCGCACGGCTGGCCGAACTGGGCATTAGCGCGCAGACAGACGTGGTGTTGCCGGATGCGGTGCGCCTGCACA
GCGCCGTGCCGGTGAGCCAGTTGCCGGGCTTTGCCGACGGCGATGTTTCGGTGCAGGACGGCTCTGCGCAGCAGGTGGCC
GATGCGCTGACGCTGGCGCCGTCGGCACGCGTGCTAGATGCCTGCGCCGCGCCGGGCGGCAAGGCGGCGCATCTGCTGGA
ACGCCATCCCGGGTTGCAGCTCGCCGCGCTTGATGTGGATGCGCGTCGCCTGGAGCGCGTGCAGCAGACCTTGCAGCGCA
GCGTGCCGAGCGCACAGGTCGCATTGCAGGCAGCCGACGCCGCCGACACGGATGCATGGTGGGACGGCCAACCGTTCGAT
GCGGTGTTGCTGGATGCGCCCTGTTCGGCCACCGGCGTAGTGCGGCGTCAACCGGATGTGCTGCTGCACCGGCGTGCCGA
CGATATCGATGCATTGTGCGCGCTGCAGGCGCGGCTGCTGGATGCGACCTGGCGCACGCTGCGCCCGGGTGGGCAGTTGC
TGTACACCACCTGCTCGCTGTTGGCGCGCGAGAACCAGACCCAGGTCGACGCATTCCTGCAACGCACCCCTGACGCACAG
GCGCAGCCCCTGGGTGCGCAGTTCGGGCATGCAGCCGGGCCCGGGCGTCAGCGCTTTCCAGGCGAGCAGCACTGCGACGG
ATTTTTCTACGCGCTTTTGCTAAAAGCCTCCTGA

Upstream 100 bases:

>100_bases
AGGGTGCGCTGCGGGTGCGGGTGCTCCAGCGCGAGGGGGGCAAGGCGATCACCGCCGCCGATTATCTCAACGCGCGGCGC
GACCTGCTGGCGCTGCGCTG

Downstream 100 bases:

>100_bases
CCGCGCGCGGCGTCCAGCGCTCCACTCTCCGCCTCCGACTGCCGCCCGACATGCTCAAGAACCGCGCTTCCAACGATGTC
TGCTTGCTGGCCATGACCGC

Product: Sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSAETSTAAGIASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERPLPPR
DAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVSDDAGWPSWLRKQVRADWGE
RAEGIFIASAQMAPMWLRVQHSRIDPAAYVARLAELGISAQTDVVLPDAVRLHSAVPVSQLPGFADGDVSVQDGSAQQVA
DALTLAPSARVLDACAAPGGKAAHLLERHPGLQLAALDVDARRLERVQQTLQRSVPSAQVALQAADAADTDAWWDGQPFD
AVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQTQVDAFLQRTPDAQ
AQPLGAQFGHAAGPGRQRFPGEQHCDGFFYALLLKAS

Sequences:

>Translated_437_residues
MSAETSTAAGIASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERPLPPR
DAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVSDDAGWPSWLRKQVRADWGE
RAEGIFIASAQMAPMWLRVQHSRIDPAAYVARLAELGISAQTDVVLPDAVRLHSAVPVSQLPGFADGDVSVQDGSAQQVA
DALTLAPSARVLDACAAPGGKAAHLLERHPGLQLAALDVDARRLERVQQTLQRSVPSAQVALQAADAADTDAWWDGQPFD
AVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQTQVDAFLQRTPDAQ
AQPLGAQFGHAAGPGRQRFPGEQHCDGFFYALLLKAS
>Mature_436_residues
SAETSTAAGIASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERPLPPRD
AELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVSDDAGWPSWLRKQVRADWGER
AEGIFIASAQMAPMWLRVQHSRIDPAAYVARLAELGISAQTDVVLPDAVRLHSAVPVSQLPGFADGDVSVQDGSAQQVAD
ALTLAPSARVLDACAAPGGKAAHLLERHPGLQLAALDVDARRLERVQQTLQRSVPSAQVALQAADAADTDAWWDGQPFDA
VLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQTQVDAFLQRTPDAQA
QPLGAQFGHAAGPGRQRFPGEQHCDGFFYALLLKAS

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=265, Percent_Identity=32.8301886792453, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI76150623, Length=265, Percent_Identity=32.8301886792453, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI32698918, Length=182, Percent_Identity=32.4175824175824, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI11545785, Length=164, Percent_Identity=35.3658536585366, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI40316918, Length=162, Percent_Identity=32.0987654320988, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI23199998, Length=257, Percent_Identity=29.9610894941634, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8922322, Length=257, Percent_Identity=29.9610894941634, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288818, Length=257, Percent_Identity=29.9610894941634, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288816, Length=257, Percent_Identity=29.9610894941634, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI39995082, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI301336155, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI2367212, Length=428, Percent_Identity=40.6542056074766, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI87081985, Length=237, Percent_Identity=33.7552742616034, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=243, Percent_Identity=30.4526748971193, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=202, Percent_Identity=31.1881188118812, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=241, Percent_Identity=27.3858921161826, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319447, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI22024126, Length=250, Percent_Identity=31.2, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=31.0344827586207, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355201, Length=230, Percent_Identity=31.304347826087, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24668781, Length=235, Percent_Identity=28.5106382978723, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21356513, Length=199, Percent_Identity=29.6482412060301, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46947; Mature: 46816

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETSTAAGIASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIR
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRPAYDVALRQWLERPLPPRDAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
QAGMVNAILRRAQREGFPAVSDDAGWPSWLRKQVRADWGERAEGIFIASAQMAPMWLRVQ
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEE
HSRIDPAAYVARLAELGISAQTDVVLPDAVRLHSAVPVSQLPGFADGDVSVQDGSAQQVA
CCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCCHHHCCCCCCCCCEECCCCHHHHH
DALTLAPSARVLDACAAPGGKAAHLLERHPGLQLAALDVDARRLERVQQTLQRSVPSAQV
HHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
ALQAADAADTDAWWDGQPFDAVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLL
HEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
DATWRTLRPGGQLLYTTCSLLARENQTQVDAFLQRTPDAQAQPLGAQFGHAAGPGRQRFP
HHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCC
GEQHCDGFFYALLLKAS
CCHHCCHHHHHHHEECC
>Mature Secondary Structure 
SAETSTAAGIASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
RRPAYDVALRQWLERPLPPRDAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPR
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
QAGMVNAILRRAQREGFPAVSDDAGWPSWLRKQVRADWGERAEGIFIASAQMAPMWLRVQ
CCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEE
HSRIDPAAYVARLAELGISAQTDVVLPDAVRLHSAVPVSQLPGFADGDVSVQDGSAQQVA
CCCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCCHHHCCCCCCCCCEECCCCHHHHH
DALTLAPSARVLDACAAPGGKAAHLLERHPGLQLAALDVDARRLERVQQTLQRSVPSAQV
HHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHH
ALQAADAADTDAWWDGQPFDAVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLL
HEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHH
DATWRTLRPGGQLLYTTCSLLARENQTQVDAFLQRTPDAQAQPLGAQFGHAAGPGRQRFP
HHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCCC
GEQHCDGFFYALLLKAS
CCHHCCHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]