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The map label for this gene is def [H]

Identifier: 84622203

GI number: 84622203

Start: 596597

End: 597109

Strand: Direct

Name: def [H]

Synonym: XOO_0546

Alternate gene names: 84622203

Gene position: 596597-597109 (Clockwise)

Preceding gene: 84622193

Following gene: 84622204

Centisome position: 12.08

GC content: 61.6

Gene sequence:

>513_bases
ATGGTTTTGCTCCCAATTCTCGAGTTTCCCGACCCGCGGCTGCGCACCAAGGCCTTGCCGGTCGACTCTGCCGAGCTTGC
CAGCCAGGCGTTCCAGGCGCTGCTGGATGACATGTTCCAGACCATGTACGAGGCGCCGGGCATCGGCCTGGCTGCCAGTC
AGGTGGACGTGCACAAGCGTTTCATGGTCATCGACGTCAGCGACGAGAAGAATCTGCCGCAGGTGTTCGTCAACCCGGAA
ATCGTGAGCAAGCAGGGCGAGCAGCTGTGTCAGGAAGGCTGCCTGTCGGTACCCGGCATTTATGCCGATGTCAGCCGCGC
CGATGCGATCACGGTGCGCTATCTCGACCGCCAGGGCCAGGCGCAGGAACTGCATGCCGACGGGCTGCTGGCCGTGTGCA
TCCAGCACGAAATGGACCACCTGGACGGCAAGCTGTTCGTAGATTATCTCTCGCCGCTCAAGCGCGAAATGGTGCGCAAG
AAGCTGGCCAAACAGCGCAAGCATGTGGCTTGA

Upstream 100 bases:

>100_bases
CCGTGCACTATAGTCCAGAAACAGGGGCGCCACGCAAGCATTGCGCTAGAATGACACCGTCTTGCCGGCGCAGCGCCGGT
GTTTCCAGACGGGTAACCCC

Downstream 100 bases:

>100_bases
GGGCGGGGATTGGAGAGTCGGGATTTGGGGTTTGCAAAAGCGGGTTACTGCTGGTCTTTGAGTCTGCTGGTTTCAAATCG
TTGAGCGCTCATCTTGGTGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MVLLPILEFPDPRLRTKALPVDSAELASQAFQALLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDVSDEKNLPQVFVNPE
IVSKQGEQLCQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA

Sequences:

>Translated_170_residues
MVLLPILEFPDPRLRTKALPVDSAELASQAFQALLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDVSDEKNLPQVFVNPE
IVSKQGEQLCQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA
>Mature_170_residues
MVLLPILEFPDPRLRTKALPVDSAELASQAFQALLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDVSDEKNLPQVFVNPE
IVSKQGEQLCQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=32.0987654320988, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=53.6585365853659, Blast_Score=180, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=31.8471337579618, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19042; Mature: 19042

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLPILEFPDPRLRTKALPVDSAELASQAFQALLDDMFQTMYEAPGIGLAASQVDVHKR
CEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FMVIDVSDEKNLPQVFVNPEIVSKQGEQLCQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEECCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEEECCCCC
AQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
HHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVLLPILEFPDPRLRTKALPVDSAELASQAFQALLDDMFQTMYEAPGIGLAASQVDVHKR
CEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FMVIDVSDEKNLPQVFVNPEIVSKQGEQLCQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEECCCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCEEEEEEECCCCC
AQELHADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
HHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]