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The map label for this gene is exoA [H]

Identifier: 84622132

GI number: 84622132

Start: 513102

End: 513902

Strand: Direct

Name: exoA [H]

Synonym: XOO_0475

Alternate gene names: 84622132

Gene position: 513102-513902 (Clockwise)

Preceding gene: 84622122

Following gene: 84622133

Centisome position: 10.39

GC content: 64.17

Gene sequence:

>801_bases
ATGCGCATCATCAGTTTTAACGCCAACGGCCTGCGCTCGGCCGCCAGCAAGGGTTTCTTCGCGTGGTTCGCCGCGCAGGA
CGCCGATGTGCTGTGCGTGCAGGAGACCAAGGCCCAGGAGCACCAACTGGCCGGGCCGGACTTCCTGCCCACCGGCTACA
AGGCTTGGTTCCGCGATGCCAGCACTAAAAAGGGCTACAGCGGCGTGGCAATCTACAGCCGGCACGAGCCCGACGAGGTG
CGTACCGCGCTGGGCTGGCCGGAATTCGACGAAGAAGGACGCTATATCGAAGCGCGCTTCGGCAACCGCAGCGTGGTGTC
CTTCTACATCCCCTCCGGTTCCTCGGGCGAGTTGCGCCAGGGCTACAAGTTCCAGGTGATGCAATGGCTGCGGCCGATCC
TGGACGAATGGCTGGCCAGCGGCCGCCAGTACGTGCTGTGTGGCGACTGGAACATCGTGCGCACGGCGCTGGACATCAAG
AACTGGAAATCCAACCAGAAGAACTCCGGCTGCCTGCCACCCGAGCGCGACTGGCTCAACGGTCTGTGCGCCGACGTGCC
AGAAGACGCCAACGCCGCCGAAGGCCGCGGCTGGGTCGACAGCTACCGCGTGCTGCATCCACAAGGCCAGGACTACACCT
GGTGGAGCAACCGCGGCGCCGCGCGCACCAACAATGTCGGTTGGCGTATCGACTACCAACTGGTCACCCCCGGCCTGCGC
GACACCTTGAGGGCTTGCTCGATCTACCGCGAAGAGCGCTTCTCCGACCACGCACCGTACATAGTGGATTACGCGCAGTG
A

Upstream 100 bases:

>100_bases
GCGATTGGGAAGCATAGCCGGTCGCGCCTTGCGGCATCCGCTGATGCGGTACGCTTTCACGAATCTCCAATCCCCATTCC
CGAATCCCGGCTCTTCATCT

Downstream 100 bases:

>100_bases
GGCAGGCCAAGGCAGGTGTTTCCGTCATAGCGCGCCAGCATGTTGCCGCACGGGGCCAAGACTTCGCCGGCAACATTGCG
ATCTGGCGGCACCTTGGCCA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGVAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNRSVVSFYIPSGSSGELRQGYKFQVMQWLRPILDEWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADVPEDANAAEGRGWVDSYRVLHPQGQDYTWWSNRGAARTNNVGWRIDYQLVTPGLR
DTLRACSIYREERFSDHAPYIVDYAQ

Sequences:

>Translated_266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGVAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNRSVVSFYIPSGSSGELRQGYKFQVMQWLRPILDEWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADVPEDANAAEGRGWVDSYRVLHPQGQDYTWWSNRGAARTNNVGWRIDYQLVTPGLR
DTLRACSIYREERFSDHAPYIVDYAQ
>Mature_266_residues
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDASTKKGYSGVAIYSRHEPDEV
RTALGWPEFDEEGRYIEARFGNRSVVSFYIPSGSSGELRQGYKFQVMQWLRPILDEWLASGRQYVLCGDWNIVRTALDIK
NWKSNQKNSGCLPPERDWLNGLCADVPEDANAAEGRGWVDSYRVLHPQGQDYTWWSNRGAARTNNVGWRIDYQLVTPGLR
DTLRACSIYREERFSDHAPYIVDYAQ

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=35.9848484848485, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=35.9848484848485, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=35.9848484848485, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI18375507, Length=310, Percent_Identity=26.1290322580645, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788046, Length=278, Percent_Identity=30.5755395683453, Blast_Score=101, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71989536, Length=262, Percent_Identity=26.3358778625954, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI221330655, Length=277, Percent_Identity=31.0469314079422, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17136678, Length=277, Percent_Identity=31.0469314079422, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30396; Mature: 30396

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDA
CEEEEECCCCHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHCCC
STKKGYSGVAIYSRHEPDEVRTALGWPEFDEEGRYIEARFGNRSVVSFYIPSGSSGELRQ
CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCC
GYKFQVMQWLRPILDEWLASGRQYVLCGDWNIVRTALDIKNWKSNQKNSGCLPPERDWLN
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHH
GLCADVPEDANAAEGRGWVDSYRVLHPQGQDYTWWSNRGAARTNNVGWRIDYQLVTPGLR
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCHH
DTLRACSIYREERFSDHAPYIVDYAQ
HHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MRIISFNANGLRSAASKGFFAWFAAQDADVLCVQETKAQEHQLAGPDFLPTGYKAWFRDA
CEEEEECCCCHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHCCCCCCCCCCHHHHHCCC
STKKGYSGVAIYSRHEPDEVRTALGWPEFDEEGRYIEARFGNRSVVSFYIPSGSSGELRQ
CCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCC
GYKFQVMQWLRPILDEWLASGRQYVLCGDWNIVRTALDIKNWKSNQKNSGCLPPERDWLN
CHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCHHHHH
GLCADVPEDANAAEGRGWVDSYRVLHPQGQDYTWWSNRGAARTNNVGWRIDYQLVTPGLR
HHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCCCCCCCCEEEEEEEECCCHH
DTLRACSIYREERFSDHAPYIVDYAQ
HHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]