| Definition | Methanosphaera stadtmanae DSM 3091 chromosome, complete genome. |
|---|---|
| Accession | NC_007681 |
| Length | 1,767,403 |
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The map label for this gene is ppsA
Identifier: 84489149
GI number: 84489149
Start: 387076
End: 389373
Strand: Direct
Name: ppsA
Synonym: Msp_0328
Alternate gene names: 84489149
Gene position: 387076-389373 (Clockwise)
Preceding gene: 84489148
Following gene: 84489150
Centisome position: 21.9
GC content: 31.85
Gene sequence:
>2298_bases ATGAATTATGTTAAAGTTTTTGAAAAATTAGGTAAGGAAGACATACCTATAGCAGGAGGTAAAGGTGCAAATCTTGGTGA ATTAACAAATGCAGGAATTCCTGTACCACCAGGTTTTGTAATAACCTCTGAAACATACAATAAATTCATAACTAAAACTG GAATAATCAATCAAATTAATGATATGTTAAATGGTTTAGATATTAATGATACATTAGAATTACAAAAAGTAGCTGATGAT ATTAAAAATCTAATTATAACAACAGACATACCAGATGATATTCAACGTGTAATTATTGAAGCTTATAATGCGTTATGTTT AAAAGTAGATTTAGATGAAGTAGTAGTAGCAATAAGATCATCTGCAACAGCAGAAGATTTACCTGATGCTTCATTTGCAG GACAACAAGAAACATATTTAAATATTTCTGGAATAGATGATGTTTTAGAAAATGTAAGAAAATGTTGGGCTTCTTTATTT GAAGCAAGAGCAATATTTTACAGAGAAGAAAATGAATTTGATCATTCAAAAGTATTAATTGCAGTTGTAGTTCAACAAAT GGTTGACTCTGAAAAAGCAGGTGTAATGTTCACTGTAGATCCATCTACAGGAGCAGAAGAAATGCTAATTGAAGGAGCAT GGGGACTTGGAGAAGGTGTAGTTTCTGGTATAGTAACTCCAGATACTTGTCGTTATGATAAAATAAATGATAAAGTAAAA TCATACCTTGTAAATACTAAAAAAACCATGTTTACTAAAGATTCTAAAACAGGTTCAACAATTCAAATAGATGTTCCAGA TGATCTAAAAGATAAAAAAGTTTTAGAAGATAGTGATATTGAACAATTAGTAACACTTGGTAGAAGAATACAAAAACATT ATGGTGCTCCAATGGACACTGAATGGGGTGTTGAAAATGGTAAAGTTTACATGTTACAAGCAAGACCTATTACAACACTT GATGTTATTCCAGAAGATACTCAATCTCTTGATGATGAAGAAAGAGTAATTATTACACGTGGTTTAGGTGCAAGTCCTGG ATTAGTATCAGGTACAGTTAAAGTTATTAAAGAATTAGATGAACTTGATAAAATTCTAGATGGAGATATACTTGTAACTA CCATGACAACACCTGATATGGTACCTGCTATGAAAAGAGCAAATGGTATTGTAACAGATGAAGGTGGAGTAACATGTCAT GCAGCAATTATTTCAAGAGAACTTGGAATTCCATGTGTATCTGGAACAGGTGAAGCTACTTCAGTTCTTAAAGAAAATAC TAAAGTTACAATCGATGGTAAAAAGGGTATAGTGTATGAAGGAGACTTTGGTGGAGATAAAGATTCTGAAGAATCAACAA CCACTCAAACAAATGTATCTGCAGCTCCATTAATAACAGTTACTGATGTAAAAGTAAATGTAAGTATGGCAGAAGCAGCT AAAAAAGCATATGCTACTGGTGCTGATGGAGTAGGATTACTTAGAACTGAACATATGATGTTAGCAACAGGAACAGTACC ATATAAATTCATAGATGAAGGAAGAGAAGATGAACTTGTAAAAGTATTAGTGGATAATATTTTAAAAGTAGTTGATGTTT TCTATCCAAAAACAGTATGGTATAGAACACTTGATGCACCAACTGATGAATTCAAAACATTAGAAGGTGGAGAAAATGAA CCTGATGAAGCAAATCCTATGCTTGGTTGGAGAGGAATAAGAAGGGAAATTGATCAACCTGATATTCTTAAAGCAGAATT TAAGGCAATTAAAAAATTACATGAACAAGGTTATACTAATGTGGGTGTAATGTTACCTTTATTACATAAACCTGAAGAAT TACGTCAAGCAAAAGAAATTGCTAGAAGTGTAGGTTTAGAACCTCATAAAGATGTTGACTTTGGTATGATGGTTGAAACA CCAGCAGCTGCGATAATTATTGAAGATTTCATAGCAGAAGGTCTTGACTTTATAAGCTTTGGAACAAATGATTTAACACA ATATACCTTAGCTTTAGATAGGAATAATGAATTAGTAGCAAAACATTATTCTGAAGCACATCCAGCAATAATTAAATTAC TTAAAAGTGTAATTAAAAAATGTAAAGCAGCTGGAGTAACAACAAGTATCTGTGGTCAAGCAGGTAGTAAACCTGAAATT GTTGAAATATTAGTAGAAGCAGGTATTGATAGTATTTCTGCAAATACTGATGCAGTTCCAACAATTAGAAAAATTGTTGC TAAAGTAGAGAAAAAAATCATGCTCGAAGCAGCTAAAAAAGCATTAATGGATGAATAA
Upstream 100 bases:
>100_bases TATAACCACACTATGGTTAATTTTAATCCTTTATATGGTTATAAATTAAATTTCGAAGAAAAGTAATGGAATGAAATTAA ATTAAAGAAGAGTGTTATAG
Downstream 100 bases:
>100_bases TTACTTTAAAAGAATAAACACTCTTCACCACCCATTATTTTTTTAGAAAAATAATTACATATTTTTTTAAACATATCATT TTTTTTATAAATTATTTGGG
Product: phosphoenolpyruvate synthase
Products: NA
Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]
Number of amino acids: Translated: 765; Mature: 765
Protein sequence:
>765_residues MNYVKVFEKLGKEDIPIAGGKGANLGELTNAGIPVPPGFVITSETYNKFITKTGIINQINDMLNGLDINDTLELQKVADD IKNLIITTDIPDDIQRVIIEAYNALCLKVDLDEVVVAIRSSATAEDLPDASFAGQQETYLNISGIDDVLENVRKCWASLF EARAIFYREENEFDHSKVLIAVVVQQMVDSEKAGVMFTVDPSTGAEEMLIEGAWGLGEGVVSGIVTPDTCRYDKINDKVK SYLVNTKKTMFTKDSKTGSTIQIDVPDDLKDKKVLEDSDIEQLVTLGRRIQKHYGAPMDTEWGVENGKVYMLQARPITTL DVIPEDTQSLDDEERVIITRGLGASPGLVSGTVKVIKELDELDKILDGDILVTTMTTPDMVPAMKRANGIVTDEGGVTCH AAIISRELGIPCVSGTGEATSVLKENTKVTIDGKKGIVYEGDFGGDKDSEESTTTQTNVSAAPLITVTDVKVNVSMAEAA KKAYATGADGVGLLRTEHMMLATGTVPYKFIDEGREDELVKVLVDNILKVVDVFYPKTVWYRTLDAPTDEFKTLEGGENE PDEANPMLGWRGIRREIDQPDILKAEFKAIKKLHEQGYTNVGVMLPLLHKPEELRQAKEIARSVGLEPHKDVDFGMMVET PAAAIIIEDFIAEGLDFISFGTNDLTQYTLALDRNNELVAKHYSEAHPAIIKLLKSVIKKCKAAGVTTSICGQAGSKPEI VEILVEAGIDSISANTDAVPTIRKIVAKVEKKIMLEAAKKALMDE
Sequences:
>Translated_765_residues MNYVKVFEKLGKEDIPIAGGKGANLGELTNAGIPVPPGFVITSETYNKFITKTGIINQINDMLNGLDINDTLELQKVADD IKNLIITTDIPDDIQRVIIEAYNALCLKVDLDEVVVAIRSSATAEDLPDASFAGQQETYLNISGIDDVLENVRKCWASLF EARAIFYREENEFDHSKVLIAVVVQQMVDSEKAGVMFTVDPSTGAEEMLIEGAWGLGEGVVSGIVTPDTCRYDKINDKVK SYLVNTKKTMFTKDSKTGSTIQIDVPDDLKDKKVLEDSDIEQLVTLGRRIQKHYGAPMDTEWGVENGKVYMLQARPITTL DVIPEDTQSLDDEERVIITRGLGASPGLVSGTVKVIKELDELDKILDGDILVTTMTTPDMVPAMKRANGIVTDEGGVTCH AAIISRELGIPCVSGTGEATSVLKENTKVTIDGKKGIVYEGDFGGDKDSEESTTTQTNVSAAPLITVTDVKVNVSMAEAA KKAYATGADGVGLLRTEHMMLATGTVPYKFIDEGREDELVKVLVDNILKVVDVFYPKTVWYRTLDAPTDEFKTLEGGENE PDEANPMLGWRGIRREIDQPDILKAEFKAIKKLHEQGYTNVGVMLPLLHKPEELRQAKEIARSVGLEPHKDVDFGMMVET PAAAIIIEDFIAEGLDFISFGTNDLTQYTLALDRNNELVAKHYSEAHPAIIKLLKSVIKKCKAAGVTTSICGQAGSKPEI VEILVEAGIDSISANTDAVPTIRKIVAKVEKKIMLEAAKKALMDE >Mature_765_residues MNYVKVFEKLGKEDIPIAGGKGANLGELTNAGIPVPPGFVITSETYNKFITKTGIINQINDMLNGLDINDTLELQKVADD IKNLIITTDIPDDIQRVIIEAYNALCLKVDLDEVVVAIRSSATAEDLPDASFAGQQETYLNISGIDDVLENVRKCWASLF EARAIFYREENEFDHSKVLIAVVVQQMVDSEKAGVMFTVDPSTGAEEMLIEGAWGLGEGVVSGIVTPDTCRYDKINDKVK SYLVNTKKTMFTKDSKTGSTIQIDVPDDLKDKKVLEDSDIEQLVTLGRRIQKHYGAPMDTEWGVENGKVYMLQARPITTL DVIPEDTQSLDDEERVIITRGLGASPGLVSGTVKVIKELDELDKILDGDILVTTMTTPDMVPAMKRANGIVTDEGGVTCH AAIISRELGIPCVSGTGEATSVLKENTKVTIDGKKGIVYEGDFGGDKDSEESTTTQTNVSAAPLITVTDVKVNVSMAEAA KKAYATGADGVGLLRTEHMMLATGTVPYKFIDEGREDELVKVLVDNILKVVDVFYPKTVWYRTLDAPTDEFKTLEGGENE PDEANPMLGWRGIRREIDQPDILKAEFKAIKKLHEQGYTNVGVMLPLLHKPEELRQAKEIARSVGLEPHKDVDFGMMVET PAAAIIIEDFIAEGLDFISFGTNDLTQYTLALDRNNELVAKHYSEAHPAIIKLLKSVIKKCKAAGVTTSICGQAGSKPEI VEILVEAGIDSISANTDAVPTIRKIVAKVEKKIMLEAAKKALMDE
Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]
COG id: COG0574
COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the PEP-utilizing enzyme family [H]
Homologues:
Organism=Escherichia coli, GI1787994, Length=792, Percent_Identity=36.1111111111111, Blast_Score=426, Evalue=1e-120, Organism=Escherichia coli, GI1788756, Length=444, Percent_Identity=31.3063063063063, Blast_Score=180, Evalue=3e-46, Organism=Escherichia coli, GI1789193, Length=385, Percent_Identity=29.8701298701299, Blast_Score=155, Evalue=1e-38, Organism=Escherichia coli, GI48994992, Length=388, Percent_Identity=26.5463917525773, Blast_Score=141, Evalue=1e-34, Organism=Escherichia coli, GI1788726, Length=414, Percent_Identity=25.6038647342995, Blast_Score=140, Evalue=3e-34, Organism=Caenorhabditis elegans, GI17564524, Length=327, Percent_Identity=31.4984709480122, Blast_Score=127, Evalue=2e-29,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013815 - InterPro: IPR013816 - InterPro: IPR008279 - InterPro: IPR006319 - InterPro: IPR018274 - InterPro: IPR000121 - InterPro: IPR002192 - InterPro: IPR015813 [H]
Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]
EC number: =2.7.9.2 [H]
Molecular weight: Translated: 83516; Mature: 83516
Theoretical pI: Translated: 4.46; Mature: 4.46
Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNYVKVFEKLGKEDIPIAGGKGANLGELTNAGIPVPPGFVITSETYNKFITKTGIINQIN CCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH DMLNGLDINDTLELQKVADDIKNLIITTDIPDDIQRVIIEAYNALCLKVDLDEVVVAIRS HHHCCCCCCCHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHC SATAEDLPDASFAGQQETYLNISGIDDVLENVRKCWASLFEARAIFYREENEFDHSKVLI CCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHH AVVVQQMVDSEKAGVMFTVDPSTGAEEMLIEGAWGLGEGVVSGIVTPDTCRYDKINDKVK HHHHHHHHCCCCCCEEEEECCCCCHHHHHEECCCCCCHHHHHCCCCCCCCCCCHHHHHHH SYLVNTKKTMFTKDSKTGSTIQIDVPDDLKDKKVLEDSDIEQLVTLGRRIQKHYGAPMDT HHHHHCHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC EWGVENGKVYMLQARPITTLDVIPEDTQSLDDEERVIITRGLGASPGLVSGTVKVIKELD CCCCCCCEEEEEECCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH ELDKILDGDILVTTMTTPDMVPAMKRANGIVTDEGGVTCHAAIISRELGIPCVSGTGEAT HHHHHHCCCEEEEEECCCHHHHHHHHCCCCEECCCCCEEEHHHHHHHCCCEEECCCCHHH SVLKENTKVTIDGKKGIVYEGDFGGDKDSEESTTTQTNVSAAPLITVTDVKVNVSMAEAA HHHHCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEHHHHHH KKAYATGADGVGLLRTEHMMLATGTVPYKFIDEGREDELVKVLVDNILKVVDVFYPKTVW HHHHCCCCCCCEEEEECCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHE YRTLDAPTDEFKTLEGGENEPDEANPMLGWRGIRREIDQPDILKAEFKAIKKLHEQGYTN EEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC VGVMLPLLHKPEELRQAKEIARSVGLEPHKDVDFGMMVETPAAAIIIEDFIAEGLDFISF CHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEC GTNDLTQYTLALDRNNELVAKHYSEAHPAIIKLLKSVIKKCKAAGVTTSICGQAGSKPEI CCCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHH VEILVEAGIDSISANTDAVPTIRKIVAKVEKKIMLEAAKKALMDE HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MNYVKVFEKLGKEDIPIAGGKGANLGELTNAGIPVPPGFVITSETYNKFITKTGIINQIN CCHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH DMLNGLDINDTLELQKVADDIKNLIITTDIPDDIQRVIIEAYNALCLKVDLDEVVVAIRS HHHCCCCCCCHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHCCEEEEECHHHHHHHHHC SATAEDLPDASFAGQQETYLNISGIDDVLENVRKCWASLFEARAIFYREENEFDHSKVLI CCCHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHH AVVVQQMVDSEKAGVMFTVDPSTGAEEMLIEGAWGLGEGVVSGIVTPDTCRYDKINDKVK HHHHHHHHCCCCCCEEEEECCCCCHHHHHEECCCCCCHHHHHCCCCCCCCCCCHHHHHHH SYLVNTKKTMFTKDSKTGSTIQIDVPDDLKDKKVLEDSDIEQLVTLGRRIQKHYGAPMDT HHHHHCHHHEEECCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC EWGVENGKVYMLQARPITTLDVIPEDTQSLDDEERVIITRGLGASPGLVSGTVKVIKELD CCCCCCCEEEEEECCCCEEEECCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH ELDKILDGDILVTTMTTPDMVPAMKRANGIVTDEGGVTCHAAIISRELGIPCVSGTGEAT HHHHHHCCCEEEEEECCCHHHHHHHHCCCCEECCCCCEEEHHHHHHHCCCEEECCCCHHH SVLKENTKVTIDGKKGIVYEGDFGGDKDSEESTTTQTNVSAAPLITVTDVKVNVSMAEAA HHHHCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEHHHHHH KKAYATGADGVGLLRTEHMMLATGTVPYKFIDEGREDELVKVLVDNILKVVDVFYPKTVW HHHHCCCCCCCEEEEECCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHE YRTLDAPTDEFKTLEGGENEPDEANPMLGWRGIRREIDQPDILKAEFKAIKKLHEQGYTN EEECCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC VGVMLPLLHKPEELRQAKEIARSVGLEPHKDVDFGMMVETPAAAIIIEDFIAEGLDFISF CHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCEEEC GTNDLTQYTLALDRNNELVAKHYSEAHPAIIKLLKSVIKKCKAAGVTTSICGQAGSKPEI CCCCCEEEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHH VEILVEAGIDSISANTDAVPTIRKIVAKVEKKIMLEAAKKALMDE HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9371463 [H]