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The map label for this gene is mfnA

Identifier: 84489150

GI number: 84489150

Start: 389662

End: 390831

Strand: Direct

Name: mfnA

Synonym: Msp_0329

Alternate gene names: 84489150

Gene position: 389662-390831 (Clockwise)

Preceding gene: 84489149

Following gene: 84489153

Centisome position: 22.05

GC content: 29.66

Gene sequence:

>1170_bases
ATGTTTGATAAAGGAAGAAGTAAGGAAGATGTATTTAGGGATTTAAATGTTTTTCATAATATGGATATGAAGTATAGTTC
AGGTAGAATATTAGGTTCTATGTGTACAAAACCAGATCCTGTGGGATTAGAAGCATATAAAATGTTTATTGAAACTAATC
TTGGGGATCCTGGTTTGTTTAAAGGTACTGCTTTAATGGAACAAGAGGTTATTAATTCTCTTGGTAACTTATTACATCTA
AAGAATCCTTGTGGTCATATTGTAACTGGTGGTACTGAGGCTAATATTATGGCAATGTGTGTTGCAAAGTATCTATATGA
AGAAGAAAATGAGGGTACTCCTGAGTTAATTCTACCAAAAAGTGCTCATTTTTCATTTAAAAAGGTACTGTCTATGTTGT
CTGTTAAACCAGTTTATGTTCCATTGAATAATGAATATAAAATAGATGTTACAAAATTACCTGATTTAATAACTGATAAT
ACCATGGCAATGGTTGGTATTGCTGGTACTACTGAGTTAGGATTGGTTGATGATATTCCCGAAATATCAAAAATTGCAAA
ATCATATGGAGTATATCTACATGTGGATGCTGCACTTGGTGGATTTATAATACCCTTTTTAAATTATAAAAATAACAATC
AATTAAACTTTGACTTTAAGTGTAAAGGTGTATCTTCCATAACTATAGATCCACATAAAATGGGACTTGCACCAGTTCCA
TCAGGTGGAATTATATTTAGGAAGAAAAAATATCTTGAAAAATTATCAATTAAAACACCATACCTAACAAAGGACAAACA
AACAACAATAGTAGGTACAAGAACAGGAGCATCAACAGCAGCAACATGGACATTATTAAACTACCATGGAATGGAAGGAT
ATAAAAAAATAGTTGAAAAAGTAATAAATCTAACAACATACACATACAACAAATTAAATAAAAATAAACACGTTACAATA
ATACATAAACCAGAATTAAATATAATATCATTTAAGGTAGACAATATTGATGTTGACACACTACAAAAACAACTACAGGC
ATATGGATGGATAGTATCATTGGCTGAATATCCTCATGTAATTAGATTAGTTTTAATGCCACATATAAAAAAAGAACATA
TTGATGAATTTCTAGTGGATCTTGATATAATAATACAAAAAAATAGATAG

Upstream 100 bases:

>100_bases
TCTATTTTTTTGTATCATGTTGTTGTCTAAGATATATCTATTAAAAACTATATATTTTATAATGATTATATTAACTAAAA
TTTTTTTTATAGGTTAAAGA

Downstream 100 bases:

>100_bases
TGGTTTTAGTAGTGAATAAGATTTATCCACCATAGATTACTTTAATGATTTCTAATTCATCACCATTTTTTACTTTTTCA
TCTTCAGTTACAGTATCACC

Product: MfnA

Products: NA

Alternate protein names: TDC

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MFDKGRSKEDVFRDLNVFHNMDMKYSSGRILGSMCTKPDPVGLEAYKMFIETNLGDPGLFKGTALMEQEVINSLGNLLHL
KNPCGHIVTGGTEANIMAMCVAKYLYEEENEGTPELILPKSAHFSFKKVLSMLSVKPVYVPLNNEYKIDVTKLPDLITDN
TMAMVGIAGTTELGLVDDIPEISKIAKSYGVYLHVDAALGGFIIPFLNYKNNNQLNFDFKCKGVSSITIDPHKMGLAPVP
SGGIIFRKKKYLEKLSIKTPYLTKDKQTTIVGTRTGASTAATWTLLNYHGMEGYKKIVEKVINLTTYTYNKLNKNKHVTI
IHKPELNIISFKVDNIDVDTLQKQLQAYGWIVSLAEYPHVIRLVLMPHIKKEHIDEFLVDLDIIIQKNR

Sequences:

>Translated_389_residues
MFDKGRSKEDVFRDLNVFHNMDMKYSSGRILGSMCTKPDPVGLEAYKMFIETNLGDPGLFKGTALMEQEVINSLGNLLHL
KNPCGHIVTGGTEANIMAMCVAKYLYEEENEGTPELILPKSAHFSFKKVLSMLSVKPVYVPLNNEYKIDVTKLPDLITDN
TMAMVGIAGTTELGLVDDIPEISKIAKSYGVYLHVDAALGGFIIPFLNYKNNNQLNFDFKCKGVSSITIDPHKMGLAPVP
SGGIIFRKKKYLEKLSIKTPYLTKDKQTTIVGTRTGASTAATWTLLNYHGMEGYKKIVEKVINLTTYTYNKLNKNKHVTI
IHKPELNIISFKVDNIDVDTLQKQLQAYGWIVSLAEYPHVIRLVLMPHIKKEHIDEFLVDLDIIIQKNR
>Mature_389_residues
MFDKGRSKEDVFRDLNVFHNMDMKYSSGRILGSMCTKPDPVGLEAYKMFIETNLGDPGLFKGTALMEQEVINSLGNLLHL
KNPCGHIVTGGTEANIMAMCVAKYLYEEENEGTPELILPKSAHFSFKKVLSMLSVKPVYVPLNNEYKIDVTKLPDLITDN
TMAMVGIAGTTELGLVDDIPEISKIAKSYGVYLHVDAALGGFIIPFLNYKNNNQLNFDFKCKGVSSITIDPHKMGLAPVP
SGGIIFRKKKYLEKLSIKTPYLTKDKQTTIVGTRTGASTAATWTLLNYHGMEGYKKIVEKVINLTTYTYNKLNKNKHVTI
IHKPELNIISFKVDNIDVDTLQKQLQAYGWIVSLAEYPHVIRLVLMPHIKKEHIDEFLVDLDIIIQKNR

Specific function: Specifically catalyzes the decarboxylation of L-tyrosine to produce tyramine

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Archaeal L-tyrosine decarboxylase subfamily

Homologues:

Organism=Homo sapiens, GI31982936, Length=388, Percent_Identity=30.6701030927835, Blast_Score=188, Evalue=6e-48,
Organism=Homo sapiens, GI197383062, Length=294, Percent_Identity=25.5102040816327, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI58331246, Length=338, Percent_Identity=24.2603550295858, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI197276620, Length=281, Percent_Identity=23.8434163701068, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4503875, Length=281, Percent_Identity=23.8434163701068, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543922, Length=384, Percent_Identity=28.90625, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17557272, Length=395, Percent_Identity=27.3417721518987, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25148342, Length=351, Percent_Identity=25.6410256410256, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320500, Length=389, Percent_Identity=27.2493573264781, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323905, Length=408, Percent_Identity=21.3235294117647, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21355963, Length=389, Percent_Identity=29.8200514138817, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24654344, Length=389, Percent_Identity=29.8200514138817, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17136594, Length=297, Percent_Identity=24.5791245791246, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356415, Length=342, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): MFNA_METST (Q2NHY7)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447382.1
- ProteinModelPortal:   Q2NHY7
- STRING:   Q2NHY7
- GeneID:   3854957
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0329
- NMPDR:   fig|339860.6.peg.320
- eggNOG:   arNOG04425
- HOGENOM:   HBG497871
- OMA:   ILGSMCT
- PhylomeDB:   Q2NHY7
- BioCyc:   MSTA339860:MSP_0329-MONOMER
- HAMAP:   MF_01610
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR020931
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11999
- TIGRFAMs:   TIGR03812

Pfam domain/function: PF00282 Pyridoxal_deC; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.1.25

Molecular weight: Translated: 43720; Mature: 43720

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00392 DDC_GAD_HDC_YDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKGRSKEDVFRDLNVFHNMDMKYSSGRILGSMCTKPDPVGLEAYKMFIETNLGDPGLF
CCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEHHCCCCCCCCHHHEEEEEECCCCCCCCC
KGTALMEQEVINSLGNLLHLKNPCGHIVTGGTEANIMAMCVAKYLYEEENEGTPELILPK
CCHHHHHHHHHHHHHHHEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEECC
SAHFSFKKVLSMLSVKPVYVPLNNEYKIDVTKLPDLITDNTMAMVGIAGTTELGLVDDIP
CCCCCHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHCCCCEEEEEECCCCCCCCCCCCH
EISKIAKSYGVYLHVDAALGGFIIPFLNYKNNNQLNFDFKCKGVSSITIDPHKMGLAPVP
HHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCEEEEECHHHCCCCCCC
SGGIIFRKKKYLEKLSIKTPYLTKDKQTTIVGTRTGASTAATWTLLNYHGMEGYKKIVEK
CCCEEEEHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHH
VINLTTYTYNKLNKNKHVTIIHKPELNIISFKVDNIDVDTLQKQLQAYGWIVSLAEYPHV
HHHHHHEEHHHCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHH
IRLVLMPHIKKEHIDEFLVDLDIIIQKNR
HHHHHHCCCCHHHHHHHHHEEEEEEECCC
>Mature Secondary Structure
MFDKGRSKEDVFRDLNVFHNMDMKYSSGRILGSMCTKPDPVGLEAYKMFIETNLGDPGLF
CCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEHHCCCCCCCCHHHEEEEEECCCCCCCCC
KGTALMEQEVINSLGNLLHLKNPCGHIVTGGTEANIMAMCVAKYLYEEENEGTPELILPK
CCHHHHHHHHHHHHHHHEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEECC
SAHFSFKKVLSMLSVKPVYVPLNNEYKIDVTKLPDLITDNTMAMVGIAGTTELGLVDDIP
CCCCCHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHCCCCEEEEEECCCCCCCCCCCCH
EISKIAKSYGVYLHVDAALGGFIIPFLNYKNNNQLNFDFKCKGVSSITIDPHKMGLAPVP
HHHHHHHHCCEEEEEECCCCCEEEEEEECCCCCEEEEEEEECCCEEEEECHHHCCCCCCC
SGGIIFRKKKYLEKLSIKTPYLTKDKQTTIVGTRTGASTAATWTLLNYHGMEGYKKIVEK
CCCEEEEHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHH
VINLTTYTYNKLNKNKHVTIIHKPELNIISFKVDNIDVDTLQKQLQAYGWIVSLAEYPHV
HHHHHHEEHHHCCCCCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCHHH
IRLVLMPHIKKEHIDEFLVDLDIIIQKNR
HHHHHHCCCCHHHHHHHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA