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The map label for this gene is dusB [H]

Identifier: 83720266

GI number: 83720266

Start: 1397711

End: 1398778

Strand: Reverse

Name: dusB [H]

Synonym: BTH_I1252

Alternate gene names: 83720266

Gene position: 1398778-1397711 (Counterclockwise)

Preceding gene: 83718864

Following gene: 83721187

Centisome position: 36.72

GC content: 67.32

Gene sequence:

>1068_bases
ATGCCCGTCCTAGGCTCTCACGTCCTGCGCAACAACCTGTTCGTCGCCCCGATGGCGGGCGTCACGGACCGGCCGTTCCG
CCAGCTCTGCAAGCGGCTCGGCGCGGGCTATGCGGTGTCGGAGATGGTCGCGTCGAACGCGCAGCTGTGGAAGAGCGAGA
AGACGATGCGCCGCGCGAACCACGCGGGCGAGGTCGAGCCGATCGCGGTGCAGATCGCGGGCGCCGATCCGGCGATGATG
GCCGAGGCCGCGCGTCACAACGTCGCGAACGGCGCGCAGATCATCGACATCAACATGGGCTGCCCGGCGAAGAAGGTTTG
CAACGTCGCCGCCGGCTCGGCGCTGCTGCAGAACGAGCCGCTCGTGCAGCGAATCGTCGAGGCGGTCGTCGACGCGGTGG
GCACGGGCCCTGACGCCGTGCCCGTCACGCTGAAGATCCGCACCGGCTGGGACCGCGAGCACAAGAACGCCGTCAAGATC
GCGAAGCTCGCCGAGGCGGCCGGCATCTCGATGCTGACCGTGCACGGCCGCACCCGCGCGGACCTGTACCGCGGCGACGC
GGAATACGACACGATCGCGGCCGTGAAGGCCGCCGTGCGCATTCCTGTCGTCGCGAACGGCGACATCACGTCGCCGCAAA
AAGCGAAGGCCGTGCTCGACGCGACGGGCGCCGACGCGCTGATGATCGGCCGCGCCGCGCAAGGAAGGCCCTGGCTCTTT
CGCGAAATCGATCATTTCCTGCAAGGCGGCGAGCTCCTGCCGCCGCCGCGCATCGACGAAATCCGCCAGGTGATGAACGA
GCACCTCGAGGATCACTACGCGTTCTACGGCGAATTCACGGGAGTCCGTACTGCGCGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGCGCAAATGGGTTCCGGCACCGGATGAACACGCTCGAAACCACCCGCGAGCAGCTTGCCGCCGTCAAC
GAATTCTTCGACGCGCAACAGGCGCTGTCCGACCGCCTCGTCTATGTGAACGACGAAGACGGCGGCGGCCGCGACGAGCC
GGACGATTCTAACCAGTTAGCAGCATGA

Upstream 100 bases:

>100_bases
ACAGTTGCCTAATTCGGCGGCACCGGCGGTTACGGTAAAATGCGCGTTTTCCCTTTCGCCGACTCCCCGCCCGCGCCCCG
CGCGGGCGGCTCCCATTGCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGACAGCCTGGACGGCTACTTCCGGGATCTCGACGGCTCCAATCCGCACGAC
GTCTATGAAATGGTGATGTC

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVDAVGTGPDAVPVTLKIRTGWDREHKNAVKI
AKLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPQKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQGGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVN
EFFDAQQALSDRLVYVNDEDGGGRDEPDDSNQLAA

Sequences:

>Translated_355_residues
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMM
AEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVDAVGTGPDAVPVTLKIRTGWDREHKNAVKI
AKLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPQKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQGGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVN
EFFDAQQALSDRLVYVNDEDGGGRDEPDDSNQLAA
>Mature_354_residues
PVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPIAVQIAGADPAMMA
EAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVDAVGTGPDAVPVTLKIRTGWDREHKNAVKIA
KLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPQKAKAVLDATGADALMIGRAAQGRPWLFR
EIDHFLQGGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLETTREQLAAVNE
FFDAQQALSDRLVYVNDEDGGGRDEPDDSNQLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=285, Percent_Identity=31.2280701754386, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI239788462, Length=285, Percent_Identity=31.2280701754386, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=34.2105263157895, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.4763948497854, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=57.3208722741433, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=32.3651452282158, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693211, Length=331, Percent_Identity=25.9818731117825, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=283, Percent_Identity=28.2685512367491, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=33.0357142857143, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=32.7659574468085, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=256, Percent_Identity=28.90625, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=320, Percent_Identity=27.1875, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=246, Percent_Identity=29.6747967479675, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=28.1938325991189, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24585320, Length=288, Percent_Identity=30.2083333333333, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=32.4675324675325, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=32.4675324675325, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=28.695652173913, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=28.2352941176471, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38504; Mature: 38373

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHCCCH
LVQRIVEAVVDAVGTGPDAVPVTLKIRTGWDREHKNAVKIAKLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPQKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHH
REIDHFLQGGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLETTREQLAAVNEFFDAQQALSDRLVYVNDEDGGGRDEPDDSNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVLGSHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSEKTMRRAN
CCCHHHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
HAGEVEPIAVQIAGADPAMMAEAARHNVANGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCHHHHCCCH
LVQRIVEAVVDAVGTGPDAVPVTLKIRTGWDREHKNAVKIAKLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPQKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCHHH
REIDHFLQGGELLPPPRIDEIRQVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLETTREQLAAVNEFFDAQQALSDRLVYVNDEDGGGRDEPDDSNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]