Click here to switch to the map view.

The map label for this gene is fis [C]

Identifier: 83721187

GI number: 83721187

Start: 1397481

End: 1397714

Strand: Reverse

Name: fis [C]

Synonym: BTH_I1251

Alternate gene names: 83721187

Gene position: 1397714-1397481 (Counterclockwise)

Preceding gene: 83720266

Following gene: 83720913

Centisome position: 36.69

GC content: 59.4

Gene sequence:

>234_bases
ATGAGCAAGCACAACATCGAACAATGTGTCCGCGACAGCCTGGACGGCTACTTCCGGGATCTCGACGGCTCCAATCCGCA
CGACGTCTATGAAATGGTGATGTCGTGCGTCGAAAAGCCTATGCTCGAAGTCGTGCTCGAACAGGCGGGCGGCAACCAGT
CGCTCGCCGCGGAGTACCTCGGCATCAATCGCAACACGCTGCGCAAGAAGCTGCAGCAGCACGGCCTGCTGTAG

Upstream 100 bases:

>100_bases
TCTTCGACGCGCAACAGGCGCTGTCCGACCGCCTCGTCTATGTGAACGACGAAGACGGCGGCGGCCGCGACGAGCCGGAC
GATTCTAACCAGTTAGCAGC

Downstream 100 bases:

>100_bases
GCGGCCGCCCCGTCCCCGTTTTCCTGGCTATTGGTGGTTCCATCATGATCAAGCAAGCGCTCATTTCCGTTTCCGACAAG
ACCGGCATCGTCGACTTCGC

Product: DNA-binding protein Fis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 77; Mature: 76

Protein sequence:

>77_residues
MSKHNIEQCVRDSLDGYFRDLDGSNPHDVYEMVMSCVEKPMLEVVLEQAGGNQSLAAEYLGINRNTLRKKLQQHGLL

Sequences:

>Translated_77_residues
MSKHNIEQCVRDSLDGYFRDLDGSNPHDVYEMVMSCVEKPMLEVVLEQAGGNQSLAAEYLGINRNTLRKKLQQHGLL
>Mature_76_residues
SKHNIEQCVRDSLDGYFRDLDGSNPHDVYEMVMSCVEKPMLEVVLEQAGGNQSLAAEYLGINRNTLRKKLQQHGLL

Specific function: Activates Ribosomal RNA Transcription. Plays A Direct Role In Upstream Activation Of rRNA Promoters. Binds To A Recombinational Enhancer Sequence That Is Required To Stimulate Hin-Mediated DNA Inversion. Prevents Initiation Of DNA Replication From Oric.

COG id: COG2901

COG function: function code KL; Factor for inversion stimulation Fis, transcriptional activator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transcriptional regulatory fis family [H]

Homologues:

Organism=Escherichia coli, GI1789661, Length=76, Percent_Identity=40.7894736842105, Blast_Score=72, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005412
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197 [H]

Pfam domain/function: PF02954 HTH_8 [H]

EC number: NA

Molecular weight: Translated: 8735; Mature: 8604

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHNIEQCVRDSLDGYFRDLDGSNPHDVYEMVMSCVEKPMLEVVLEQAGGNQSLAAEYL
CCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GINRNTLRKKLQQHGLL
CCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKHNIEQCVRDSLDGYFRDLDGSNPHDVYEMVMSCVEKPMLEVVLEQAGGNQSLAAEYL
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GINRNTLRKKLQQHGLL
CCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]