Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 83720145

GI number: 83720145

Start: 2101943

End: 2102800

Strand: Reverse

Name: folD [H]

Synonym: BTH_I1861

Alternate gene names: 83720145

Gene position: 2102800-2101943 (Counterclockwise)

Preceding gene: 83719547

Following gene: 83718681

Centisome position: 55.2

GC content: 68.41

Gene sequence:

>858_bases
ATGACAGCCACTCTGATCGACGGCAACGCCCTTTCGAAGACCCTGCGCGCGCAAGCGGCCAAGCGCGCCGCCGCGCTCGC
CGCGCGCGGTCACCGGCCGGGCCTCGCGGTGATCCTCGTCGGCGACAACCCGGCGAGCGAAGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCTGAAGGACCGCTATCCGGCGACGCTCTCCGAGCCCGAGCTGCTCGCG
CGCATCGGCGAACTGAACCGCGATCCGAAGATCCACGGGATTCTCGTGCAGTTGCCGCTGCCCGCGCACATCGACAGCCA
CAAGGTGATCGAGGCGATCGCGCCGGAGAAGGACGTCGACGGCTTTCACGTCGCCAACGCGGGCGCGCTGCTGACGGGCA
AGCCGCTCTTCCGCCCGTGCACGCCGTACGGCGTGATGAAGATGTTCGAAGCGTACAAGATCCCGCTGCAGGGCGCGAAC
GCGGTCGTGATCGGCCGCTCGAACATCGTCGGCAAGCCGATGGCGCTGCTGCTGCTCGAAGCGGGCGCGACCGTCACGAT
CTGCCATAGCAAGACGCGCGACCTCGCCGCGCATACGCGCGAGGCCGACATCGTCGTCGCGGCGGTCGGCAAGCGCAACG
TGCTGACCGCCGACATGGTGAAGCCGGGCGCGACCGTGATCGACGTCGGGATGAACCGCAACGACGAAGGCAAGCTGTGC
GGCGATGTCGACTTCGCGGGCGTCTCGCAGGTCGCGGGCCACATCACGCCCGTGCCGGGCGGCGTCGGCCCGATGACGAT
CACGATGCTGCTCGTCAATACGATCGAAGCCGCCGAGCGCGCCGCGGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
GTCGGCTGACGACAGGCGGGCGGTATAATTCGATGCTTCGCTGCAGCGCAACCCAGCGCCGCACGCCCGCATATCCAACC
TGCCCAAGCGAATTCCCACC

Downstream 100 bases:

>100_bases
CCGGCGGATTCGCCAACGCTCGCGGCGGCGGCGAGCCGGCCGGCTTCGGCGACAATAGCCGCGGCCGGCAAGCCCGCCGC
GCGTGCCGTCGATCTTGCAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTATLIDGNALSKTLRAQAAKRAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLA
RIGELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGAN
AVVIGRSNIVGKPMALLLLEAGATVTICHSKTRDLAAHTREADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAAAA

Sequences:

>Translated_285_residues
MTATLIDGNALSKTLRAQAAKRAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLA
RIGELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGAN
AVVIGRSNIVGKPMALLLLEAGATVTICHSKTRDLAAHTREADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAAAA
>Mature_284_residues
TATLIDGNALSKTLRAQAAKRAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDNGFFSLKDRYPATLSEPELLAR
IGELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANA
VVIGRSNIVGKPMALLLLEAGATVTICHSKTRDLAAHTREADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLCG
DVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAAAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=48.2993197278912, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.9183673469388, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.0689655172414, Blast_Score=218, Evalue=5e-57,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=29.1457286432161, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=52.3297491039427, Blast_Score=280, Evalue=9e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=41.0169491525424, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=46.8531468531469, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=42, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=293, Percent_Identity=26.6211604095563, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=50, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=50, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.0207612456747, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29911; Mature: 29780

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLIDGNALSKTLRAQAAKRAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEECCC
GFFSLKDRYPATLSEPELLARIGELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANAVVIGRSNIVGKPMALLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
AGATVTICHSKTRDLAAHTREADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
CCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCCCEE
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAAAA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATLIDGNALSKTLRAQAAKRAAALAARGHRPGLAVILVGDNPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCEEECCC
GFFSLKDRYPATLSEPELLARIGELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALLTGKPLFRPCTPYGVMKMFEAYKIPLQGANAVVIGRSNIVGKPMALLLLE
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEE
AGATVTICHSKTRDLAAHTREADIVVAAVGKRNVLTADMVKPGATVIDVGMNRNDEGKLC
CCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCCCEE
GDVDFAGVSQVAGHITPVPGGVGPMTITMLLVNTIEAAERAAAAA
CCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA