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The map label for this gene is fixJ [H]

Identifier: 83719547

GI number: 83719547

Start: 2103116

End: 2103754

Strand: Reverse

Name: fixJ [H]

Synonym: BTH_I1862

Alternate gene names: 83719547

Gene position: 2103754-2103116 (Counterclockwise)

Preceding gene: 83720581

Following gene: 83720145

Centisome position: 55.23

GC content: 64.63

Gene sequence:

>639_bases
ATGAATAGCCCTGTCACCACCTCTCAGGAAACCGTCTTTGTCGTCGACGACGACGAGGCCGTGCGCGACTCGCTGCGCTG
GCTGCTGGAGGCAAACGGCTATCGCGTGCAGTGCTTCTCGAGCGCCGAGCAGTTCCTCGAGGCGTATCAGCCCGCGCAGC
AGGCGGGACAGATCGCGTGCCTGATCCTCGACGTGCGGATGTCCGGGATGAGCGGCCTCGAGTTGCAGGAGCGTCTGATC
GCCGAGAACGCGGCGCTCCCGATCATCTTCGTGACGGGCCACGGCGACGTGCCGATGGCCGTGTCGACGATGAAGAAGGG
CGCGATGGACTTCATCGAGAAACCGTTCGACGAGGCCGAGCTGCGCAAGCTCGTCGAGCGCATGCTCGACAAGGCGCGCA
GCGAGAGCAAGAGCGTCCAGGAGCAGCGTGCGGCGAGCGAGCGCCTGTCGAAGCTGACGGCGCGCGAGCAGCAGGTGCTC
GAGCGGATCATTGCGGGCCGTCTGAACAAGCAGATCGCCGACGATCTCGGGATCAGCATCAAGACGGTCGAGGCGCACCG
CGCGAACATCATGGAAAAGCTCAACGTCAACACGGTGGCCGACCTGCTGCGGCTCGCGCTGTCGAAGAAGCAGGCGTAA

Upstream 100 bases:

>100_bases
TATCGGGCGCGACGTTCCACTGCAGCCTGCCCATTGGGGAACCGGAGGATCTCGGTCACGGATCCGAGACATCGCCATCA
CAAACCGTAACGGGAGAGAT

Downstream 100 bases:

>100_bases
CGCGCGGCGCCCGGCCCTCCTCCTCCGCCCGGCGCGCGCCGGGCGAGCGCCGGTTTTGCAGCGCGGCATCGCGCGCGATG
CTGCCGGTTCCCGTCCCGCT

Product: LuxR family DNA-binding response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLEAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA

Sequences:

>Translated_212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLEAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA
>Mature_212_residues
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLEAYQPAQQAGQIACLILDVRMSGMSGLELQERLI
AENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAELRKLVERMLDKARSESKSVQEQRAASERLSKLTAREQQVL
ERIIAGRLNKQIADDLGISIKTVEAHRANIMEKLNVNTVADLLRLALSKKQA

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=161, Percent_Identity=34.1614906832298, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=25.8373205741627, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=33.6734693877551, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=124, Percent_Identity=35.4838709677419, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786747, Length=202, Percent_Identity=24.2574257425743, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=29.6875, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=26.8292682926829, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23618; Mature: 23618

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLEAYQPAQQAGQIAC
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHCCCEEE
LILDVRMSGMSGLELQERLIAENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAE
EEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRKLVERMLDKARSESKSVQEQRAASERLSKLTAREQQVLERIIAGRLNKQIADDLGISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSKKQA
EEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSPVTTSQETVFVVDDDEAVRDSLRWLLEANGYRVQCFSSAEQFLEAYQPAQQAGQIAC
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCCHHHCCCEEE
LILDVRMSGMSGLELQERLIAENAALPIIFVTGHGDVPMAVSTMKKGAMDFIEKPFDEAE
EEEEEHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
LRKLVERMLDKARSESKSVQEQRAASERLSKLTAREQQVLERIIAGRLNKQIADDLGISI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
KTVEAHRANIMEKLNVNTVADLLRLALSKKQA
EEHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]