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The map label for this gene is prpB [H]

Identifier: 83593655

GI number: 83593655

Start: 2688282

End: 2689202

Strand: Direct

Name: prpB [H]

Synonym: Rru_A2320

Alternate gene names: 83593655

Gene position: 2688282-2689202 (Clockwise)

Preceding gene: 83593654

Following gene: 83593656

Centisome position: 61.76

GC content: 65.58

Gene sequence:

>921_bases
ATGCCCTATCTGATTGCCGACACCTTGCCAAAGGAAAGCGCGGGCACCCGCTTCCGGGCGCTTTTGAACCGCCCGGAGAT
CCTCAGACTTCCCGGCGCCCATAACGGACATGCCGCCTTGCAGGCCCGCGCGGCGGGCTTCGAGGGGGTGTATCTTTCGG
GCGCGGCGATGAGCGCCTCCATGGGGCTGCCCGATCTCGGCATCCTCACCATCGAAGACGTCGCCTTCTTCATCCGCCAG
ATCGTCCGCTCCGCCGGCTTGCCGCTGCTGGTCGATGGCGATACCGGCTATGGCGAAGCGCTCAACGTCATGCACATGGT
CCGCACCTTTGAAGAGGCCGGCGCCGGGGCGGTCCACCTGGAGGACCAACTCCTTCCCAAGAAATGCGGGCACCTCAACG
ACAAGAAACTGGCCAAAGCCGACGACATGGCGGCCAAAGTGGCGGCGGCCAGGAAAGCCGCCCGCGACATCGTGATCATC
GCCCGCACCGACGCGGCCGCCAGCGAGGGGCTGGAGGGCGCGATCGCCCGCGCCAGATTATACGTGGAAGCCGGCGCCGA
CGCGATTTTCCCCGAGGCGCTCAATACGCAAGAGATGTTCCGGGCCTTCGCCGAGCGGATGCCCGGCGTTCCCCTGTTGG
CCAATATGACCGAATTCGGCAAGACGCCCTTTTTCACCGCCACCGAATTCGCCGAGATGGGCTATGCCATGGTCATCTGG
CCGGTCTCTTCCCTGCGCGTCGCCAACAAGGCGCAGGCCGAGCTTTACGCCGCCATCGCCCGGGATGGGGGGGCCCACCG
CATGGTCGAGCGCATGCAGACCCGTGCGGAGCTTTACGCCACCATCGGCCTGCATGATTACGAGGCGCTCGACGCCTCAA
TCGTCCAGACGCTGATCCCGCAAGGCTCTCCCCACCGCTGA

Upstream 100 bases:

>100_bases
CAAGATCATCCGCCCCTCGGCCAATTACACCGGCCCGGAAAACCTCGCCTTCGTGCCCATGTCGGCCAGAAGCGACCATG
TTTCGATGTGAGGAACACAG

Downstream 100 bases:

>100_bases
TCCCGTCCATCCTCCGGTCCGGCCATCCCGTTCCCCAACAGGGGCGGGCATGGCCGGCCGTCCTCCCCGGCCCCTCCCCG
CCCCGGCATCGGCATCGGCG

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSASMGLPDLGILTIEDVAFFIRQ
IVRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHLEDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVII
ARTDAAASEGLEGAIARARLYVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIW
PVSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIPQGSPHR

Sequences:

>Translated_306_residues
MPYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSASMGLPDLGILTIEDVAFFIRQ
IVRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHLEDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVII
ARTDAAASEGLEGAIARARLYVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIW
PVSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIPQGSPHR
>Mature_305_residues
PYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSASMGLPDLGILTIEDVAFFIRQI
VRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHLEDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVIIA
RTDAAASEGLEGAIARARLYVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIWP
VSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIPQGSPHR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=280, Percent_Identity=44.6428571428571, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1790445, Length=316, Percent_Identity=29.1139240506329, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=199, Percent_Identity=31.6582914572864, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561814, Length=199, Percent_Identity=31.6582914572864, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32891; Mature: 32760

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSAS
CCEEECCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCEEEECCHHHHC
MGLPDLGILTIEDVAFFIRQIVRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHL
CCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEE
EDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVIIARTDAAASEGLEGAIARARL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHCCHHHHHHHHEE
YVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIW
EEECCCCEECCCCCCHHHHHHHHHHHCCCCCEECCHHHHCCCCCEEHHHHHHCCCEEEEE
PVSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIP
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
QGSPHR
CCCCCC
>Mature Secondary Structure 
PYLIADTLPKESAGTRFRALLNRPEILRLPGAHNGHAALQARAAGFEGVYLSGAAMSAS
CEEECCCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHHHHHCCCCCCEEEECCHHHHC
MGLPDLGILTIEDVAFFIRQIVRSAGLPLLVDGDTGYGEALNVMHMVRTFEEAGAGAVHL
CCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEE
EDQLLPKKCGHLNDKKLAKADDMAAKVAAARKAARDIVIIARTDAAASEGLEGAIARARL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHCCHHHHHHHHEE
YVEAGADAIFPEALNTQEMFRAFAERMPGVPLLANMTEFGKTPFFTATEFAEMGYAMVIW
EEECCCCEECCCCCCHHHHHHHHHHHCCCCCEECCHHHHCCCCCEEHHHHHHCCCEEEEE
PVSSLRVANKAQAELYAAIARDGGAHRMVERMQTRAELYATIGLHDYEALDASIVQTLIP
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
QGSPHR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]