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The map label for this gene is prpD1 [H]

Identifier: 83593653

GI number: 83593653

Start: 2685597

End: 2687096

Strand: Direct

Name: prpD1 [H]

Synonym: Rru_A2318

Alternate gene names: 83593653

Gene position: 2685597-2687096 (Clockwise)

Preceding gene: 83593652

Following gene: 83593654

Centisome position: 61.7

GC content: 67.2

Gene sequence:

>1500_bases
ATGAAGCTTCACGACGTTCGCACCTATAAATCCGCCGAACCGCTACCGCGCGAAAATCAATTGGCGTGGAAACTCGCCGC
CGTCGCCACGGATCGGGTGCCCGTTCCCCCCGACGTCACCGAGATGATCGGCAACCGCATCCTCGACAACGCCGCGGTCG
CCGCCGCCTCTCTCGCCCGCGGCCCGGTCCGTTCGGCCCGCGCCCAGGCCCTGGGTCATCGGTCGGAGCGGGGGGCGCCG
ATCTTCGGCTTGGCCGAAGGCGGAAAGGTCAGCCCGGAATGGGCCGCCTGGGCCAACGGCGTGGCGGTGCGCGAGCTGGA
TTTTCACGACACCTTCCTGGCCGCCGAATACAGCCATCCCGGCGACAACATCCCGCCGATCCTTGCCGTGGCGCAATCCC
TGGGATGCGACGGCGCGGCGCTGGTGCGCGGTCTGGCCGCCGGCTACGAGATCCAGGTCGATCTGGTCAAAGGCATCTGC
CTGCACGAGCACAAGATCGATCATATCGCCCATCTCGGGCCAAGCGCCGCCGGTGGCATCGGCGCCCTGCTGGGGCTGTC
CACCGAAGTCACCTATCAGGCCATCGGGCAGGCCCTTCACACCACGACGACGACCCGCCAATCCCGCAAGGGCGAGATTT
CGAGCTGGAAGGCCTATGCCCCCGCCTTCGCCGGGAAAATGGCGATCGAGGCGGTCGACCGCGCCATGCGGGGCGAAGGC
GCGCCCGCGCCGGCTTACGAGGGCGAGGACGGCTTCATCGCTTGGCTGCTGTCGGGCCCCGGCCATGTCTACCAGGTGCC
GCTGCCCGAGCCCGGCGAGGCCAAGCGGGCGATCCTCGACACCTACACCAAGGAATATTCGGCAGAATATCAAAGCCAGG
CCCTGATCGATCTGGCCAAGCGCATGGGGCCGAAAGTCGGCGACCTCTCGCAGGTCGAGCGGATCGTCATCCAGACCAGC
CATCACACCCATTACGTGATCGGCACCGGCGCCAATGATCCCCAGAAGCTCGATCCCCGCGCCAGCCGGGAAACCCTTGA
CCACTCGATCATGTACATCTTCGCCGTCGCCCTGCAGGACGGCGGCTGGCATCACGTCAAAAGCTATGCCCCCGAGCGGG
CGGGACGGCCCGACACCATCGACCTCTGGCACAAGATCACGACCGAGGAAGACCCCGCCTGGACCGCCCGCTACCACGCC
AAGGGCGCCGACAAGGCGTTTGGCGGCAAGGTGATCATCACCTTGAAGGGGGGGCGCCAGATCGTCGACGAACTGGCCGT
GGCCGATGCCCACCCCGCCGGAACCCGACCCTTCGCCCGCCCCGACTACATTGGAAAATTCCGCACCCTGGCCGAGGGCG
TCATCGCCCCCGCCGAGCAGGACCGTTTTATCGCCCTGGTCGAGAGACTGCCCGAGCTGACCGCGGACGAGGTCGCCCAC
CTGAGCTTCGTCGTCTCGCCTGACACCCTGGGGCCGGCCACGCCGCCCGGCATTTTCTAA

Upstream 100 bases:

>100_bases
CGGTTTCAGCCCTGTCCCACGCATCGCCCCTTGCCGATGATGCGACACTGCCTTCGCGGCCCTTTGGTTGCCCACTTCAA
AAAAAACGGAAGTCCAGGAC

Downstream 100 bases:

>100_bases
AAAGGGGACCGTCCGATGACCGAAACCACATTCAAGCCCAAGAAGTCCGTGGCGCTTTCGGGTATCGCCGCCGGCAATAC
GGCGCTGTGCACCGTGGGCC

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLARGPVRSARAQALGHRSERGAP
IFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHPGDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGIC
LHEHKIDHIAHLGPSAAGGIGALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAKRMGPKVGDLSQVERIVIQTS
HHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQDGGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHA
KGADKAFGGKVIITLKGGRQIVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
LSFVVSPDTLGPATPPGIF

Sequences:

>Translated_499_residues
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLARGPVRSARAQALGHRSERGAP
IFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHPGDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGIC
LHEHKIDHIAHLGPSAAGGIGALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAKRMGPKVGDLSQVERIVIQTS
HHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQDGGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHA
KGADKAFGGKVIITLKGGRQIVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
LSFVVSPDTLGPATPPGIF
>Mature_499_residues
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLARGPVRSARAQALGHRSERGAP
IFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHPGDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGIC
LHEHKIDHIAHLGPSAAGGIGALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAKRMGPKVGDLSQVERIVIQTS
HHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQDGGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHA
KGADKAFGGKVIITLKGGRQIVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
LSFVVSPDTLGPATPPGIF

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=415, Percent_Identity=23.3734939759036, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325258, Length=497, Percent_Identity=25.9557344064386, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53608; Mature: 53608

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLAR
CCCCCCCCCCCCCCCCCCCCHHEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GPVRSARAQALGHRSERGAPIFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHP
CCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCEEEEEECCCCCEEEEECCCC
GDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGICLHEHKIDHIAHLGPSAAGGI
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHHHHHHCCCCCCCHH
GALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAK
CCCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGPKVGDLSQVERIVIQTSHHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQD
HHCCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
GGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHAKGADKAFGGKVIITLKGGRQ
CCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHH
IVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHEE
LSFVVSPDTLGPATPPGIF
EEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKLHDVRTYKSAEPLPRENQLAWKLAAVATDRVPVPPDVTEMIGNRILDNAAVAAASLAR
CCCCCCCCCCCCCCCCCCCCHHEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GPVRSARAQALGHRSERGAPIFGLAEGGKVSPEWAAWANGVAVRELDFHDTFLAAEYSHP
CCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHHCCEEEEEECCCCCEEEEECCCC
GDNIPPILAVAQSLGCDGAALVRGLAAGYEIQVDLVKGICLHEHKIDHIAHLGPSAAGGI
CCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHHHHHHCCCCCCCHH
GALLGLSTEVTYQAIGQALHTTTTTRQSRKGEISSWKAYAPAFAGKMAIEAVDRAMRGEG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
APAPAYEGEDGFIAWLLSGPGHVYQVPLPEPGEAKRAILDTYTKEYSAEYQSQALIDLAK
CCCCCCCCCCCEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGPKVGDLSQVERIVIQTSHHTHYVIGTGANDPQKLDPRASRETLDHSIMYIFAVALQD
HHCCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
GGWHHVKSYAPERAGRPDTIDLWHKITTEEDPAWTARYHAKGADKAFGGKVIITLKGGRQ
CCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCHH
IVDELAVADAHPAGTRPFARPDYIGKFRTLAEGVIAPAEQDRFIALVERLPELTADEVAH
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHEE
LSFVVSPDTLGPATPPGIF
EEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11976302; 12948626 [H]