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The map label for this gene is lipB

Identifier: 82703109

GI number: 82703109

Start: 2285638

End: 2286285

Strand: Reverse

Name: lipB

Synonym: Nmul_A1988

Alternate gene names: 82703109

Gene position: 2286285-2285638 (Counterclockwise)

Preceding gene: 82703110

Following gene: 82703108

Centisome position: 71.8

GC content: 54.48

Gene sequence:

>648_bases
GTGATACAGCCCGTTAGCCTGGAGTTTGTGCCCGGTCCTTCAGGACTTGAAGTCAAATATGCTGCAGTGATGGATTATCT
TTCCATCTGGCAGGCGATGAAGGCTTTCACGGCGAGTCGCACGCAAAATACCCCTGATGAAATCTGGTTGCTGCAGCATT
GGCCGGTCTATACCCAGGGTGTTGCCGGCAAACCCGAGCATTTGCTTTGCAATCCAGGCATTCCCGTAGTACGCACGGAT
CGGGGTGGTCAAATCACCTATCACGGCCCGGGACAGATTATTGCGTACCTGTTGCTGGATATGCGGCGCCTCAAGTTAGG
CGTGCGCGACCTGGTCAGAAAAATGGAGGGCGCCGTGGTAGACTTGCTGGATGAGTATCGCGTCAACGCCTGCGGCGATG
AGGATGCGCCCGGTGTATACGTTGGCGGAGCAAAAATCGCGGCACTGGGGCTGAAGATAAAAAATGGATGCTGCTACCAT
GGCCTGGCTCTGAATGTCAGCATGGACCTTGCCCCGTTTATGGCTATCAACCCTTGCGGCTATACGGGACTAAGGGTTAC
GCAAACCAGCGATCTCGGTATAACCGACGAGCTGGAAACGCTGCAAGGAAAGCTTGCCGAAAAACTGAAGGCCAGGCTGA
AGCAATGA

Upstream 100 bases:

>100_bases
GGATGCGTTATATCAGGAACTGTGCGATCATCCCACAGTGGTAATGGTACTTTAGCGAATTTCTGATTCCTTCCGCGATT
CATATTCCGGAAGCTTCTCC

Downstream 100 bases:

>100_bases
TTTCAAGAATATTTAAAGATGGATATCAATCGATCCGGGTATTCCGGATTGATATGGATCAATATCAAGAAGACGTTTCT
TCCTTATCATGACAATTGAA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIQPVSLEFVPGPSGLEVKYAAVMDYLSIWQAMKAFTASRTQNTPDEIWLLQHWPVYTQGVAGKPEHLLCNPGIPVVRTD
RGGQITYHGPGQIIAYLLLDMRRLKLGVRDLVRKMEGAVVDLLDEYRVNACGDEDAPGVYVGGAKIAALGLKIKNGCCYH
GLALNVSMDLAPFMAINPCGYTGLRVTQTSDLGITDELETLQGKLAEKLKARLKQ

Sequences:

>Translated_215_residues
MIQPVSLEFVPGPSGLEVKYAAVMDYLSIWQAMKAFTASRTQNTPDEIWLLQHWPVYTQGVAGKPEHLLCNPGIPVVRTD
RGGQITYHGPGQIIAYLLLDMRRLKLGVRDLVRKMEGAVVDLLDEYRVNACGDEDAPGVYVGGAKIAALGLKIKNGCCYH
GLALNVSMDLAPFMAINPCGYTGLRVTQTSDLGITDELETLQGKLAEKLKARLKQ
>Mature_215_residues
MIQPVSLEFVPGPSGLEVKYAAVMDYLSIWQAMKAFTASRTQNTPDEIWLLQHWPVYTQGVAGKPEHLLCNPGIPVVRTD
RGGQITYHGPGQIIAYLLLDMRRLKLGVRDLVRKMEGAVVDLLDEYRVNACGDEDAPGVYVGGAKIAALGLKIKNGCCYH
GLALNVSMDLAPFMAINPCGYTGLRVTQTSDLGITDELETLQGKLAEKLKARLKQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=142, Percent_Identity=40.1408450704225, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87081767, Length=198, Percent_Identity=50, Blast_Score=204, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6323268, Length=151, Percent_Identity=31.7880794701987, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355483, Length=176, Percent_Identity=32.9545454545455, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_NITMU (Q2Y7I8)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412675.1
- ProteinModelPortal:   Q2Y7I8
- SMR:   Q2Y7I8
- STRING:   Q2Y7I8
- GeneID:   3785012
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1988
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   RDELISC
- PhylomeDB:   Q2Y7I8
- ProtClustDB:   PRK14342
- BioCyc:   NMUL323848:NMUL_A1988-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23528; Mature: 23528

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPVSLEFVPGPSGLEVKYAAVMDYLSIWQAMKAFTASRTQNTPDEIWLLQHWPVYTQG
CCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEECC
VAGKPEHLLCNPGIPVVRTDRGGQITYHGPGQIIAYLLLDMRRLKLGVRDLVRKMEGAVV
CCCCCCEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
DLLDEYRVNACGDEDAPGVYVGGAKIAALGLKIKNGCCYHGLALNVSMDLAPFMAINPCG
HHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEEEEEECCCCCEEECCCC
YTGLRVTQTSDLGITDELETLQGKLAEKLKARLKQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIQPVSLEFVPGPSGLEVKYAAVMDYLSIWQAMKAFTASRTQNTPDEIWLLQHWPVYTQG
CCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCEECC
VAGKPEHLLCNPGIPVVRTDRGGQITYHGPGQIIAYLLLDMRRLKLGVRDLVRKMEGAVV
CCCCCCEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
DLLDEYRVNACGDEDAPGVYVGGAKIAALGLKIKNGCCYHGLALNVSMDLAPFMAINPCG
HHHHHHCCCCCCCCCCCEEEECCCEEEEEEEEECCCCEEEEEEEEEEECCCCCEEECCCC
YTGLRVTQTSDLGITDELETLQGKLAEKLKARLKQ
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA