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The map label for this gene is lipA

Identifier: 82703108

GI number: 82703108

Start: 2284599

End: 2285549

Strand: Reverse

Name: lipA

Synonym: Nmul_A1987

Alternate gene names: 82703108

Gene position: 2285549-2284599 (Counterclockwise)

Preceding gene: 82703109

Following gene: 82703107

Centisome position: 71.78

GC content: 56.05

Gene sequence:

>951_bases
ATGACAATTGAAAGTCGCCAGAAAGGCGTTGCAAAAACCGCGCGCAATCCCGTCAAAATCGCGCCGCAATCTACCGATCA
GTTGCTGCGTAAACCTTCCTGGATCAGGGTGCGATCCTCCAATAGCCAGGAGTTTTATGAGGTCAAGCGGATTTTGCGGG
AGCAGAAGCTGCACACGGTATGTGAGGAAGCTTCCTGTCCCAACATCGGCGAATGCTTCGGCAAAGGCACTGCAACTTTC
ATGATACTGGGCGATCTCTGTACCCGGCGCTGTCCCTTCTGCGACGTGGCGCATGGCCGGCCGCGTCCTCCGGACCCCGA
AGAACCTCTGCACCTCGCGCAATCCATTGCCGCCATGAAGTTGAAATATGTGGTTATCACGAGCGTTGACCGCGATGATC
TCAGGGATGGAGGAGCTCAGCATTTCGTGGATTGCATCCGTGAGGTTCGAGCCCATTCGCCGCAGACAAAGATAGAGATC
CTGGTGCCGGATTTTCGCGGGAGACTGGATATTGCGCTGGAAAAATTATTCGCCTGCCCGCCCGATGTGATGAACCACAA
TCTCGAAACGGTACCCCGCCTCTACCGGCAATGCCGTCCAGGCGCGGACTACACGCATTCGCTCCGGTTATTGAAGGAGT
TCAAGGCACGTTTTCCGGGCATCCCCACCAAATCCGGATTGATGCTGGGACTGGGAGAAACTGACGAGGAGATCCTCGAC
GTGATGCGCGACTTGCGCAAGCATGATGTCGAGATGCTTACCATTGGCCAGTATCTGCAGCCAAGCATCGGACATCTTCC
TGTCATGCGTTATGTCACCCCGGGGGCATTTAAGGAATTTGAACGCGCCGCGATCGAAATGGGTTTCAGCAACGCCGCGT
GCGGGCCCATGGTCCGGTCCAGCTACCATGCGGATCAGCAGGCGCATGAGGCAGGCATCATACAGAGGTAG

Upstream 100 bases:

>100_bases
CTGAAGCAATGATTTCAAGAATATTTAAAGATGGATATCAATCGATCCGGGTATTCCGGATTGATATGGATCAATATCAA
GAAGACGTTTCTTCCTTATC

Downstream 100 bases:

>100_bases
CGGTTTTAGCGAAGAGAATCAGCGAGGAGAGGAATTTCAAGGCCGGTTATGCAAACCCGACAGGAGTTACTCCAATGCGC
ATCATTCCTGCTATTGTTGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTIESRQKGVAKTARNPVKIAPQSTDQLLRKPSWIRVRSSNSQEFYEVKRILREQKLHTVCEEASCPNIGECFGKGTATF
MILGDLCTRRCPFCDVAHGRPRPPDPEEPLHLAQSIAAMKLKYVVITSVDRDDLRDGGAQHFVDCIREVRAHSPQTKIEI
LVPDFRGRLDIALEKLFACPPDVMNHNLETVPRLYRQCRPGADYTHSLRLLKEFKARFPGIPTKSGLMLGLGETDEEILD
VMRDLRKHDVEMLTIGQYLQPSIGHLPVMRYVTPGAFKEFERAAIEMGFSNAACGPMVRSSYHADQQAHEAGIIQR

Sequences:

>Translated_316_residues
MTIESRQKGVAKTARNPVKIAPQSTDQLLRKPSWIRVRSSNSQEFYEVKRILREQKLHTVCEEASCPNIGECFGKGTATF
MILGDLCTRRCPFCDVAHGRPRPPDPEEPLHLAQSIAAMKLKYVVITSVDRDDLRDGGAQHFVDCIREVRAHSPQTKIEI
LVPDFRGRLDIALEKLFACPPDVMNHNLETVPRLYRQCRPGADYTHSLRLLKEFKARFPGIPTKSGLMLGLGETDEEILD
VMRDLRKHDVEMLTIGQYLQPSIGHLPVMRYVTPGAFKEFERAAIEMGFSNAACGPMVRSSYHADQQAHEAGIIQR
>Mature_315_residues
TIESRQKGVAKTARNPVKIAPQSTDQLLRKPSWIRVRSSNSQEFYEVKRILREQKLHTVCEEASCPNIGECFGKGTATFM
ILGDLCTRRCPFCDVAHGRPRPPDPEEPLHLAQSIAAMKLKYVVITSVDRDDLRDGGAQHFVDCIREVRAHSPQTKIEIL
VPDFRGRLDIALEKLFACPPDVMNHNLETVPRLYRQCRPGADYTHSLRLLKEFKARFPGIPTKSGLMLGLGETDEEILDV
MRDLRKHDVEMLTIGQYLQPSIGHLPVMRYVTPGAFKEFERAAIEMGFSNAACGPMVRSSYHADQQAHEAGIIQR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=47.3684210526316, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=47.3684210526316, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1786846, Length=298, Percent_Identity=66.1073825503356, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=298, Percent_Identity=42.2818791946309, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=313, Percent_Identity=43.4504792332268, Blast_Score=258, Evalue=6e-70,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=47.2222222222222, Blast_Score=264, Evalue=7e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_NITMU (Q2Y7I9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412674.1
- STRING:   Q2Y7I9
- GeneID:   3785011
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1987
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q2Y7I9
- ProtClustDB:   PRK05481
- BioCyc:   NMUL323848:NMUL_A1987-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35691; Mature: 35559

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIESRQKGVAKTARNPVKIAPQSTDQLLRKPSWIRVRSSNSQEFYEVKRILREQKLHTV
CCCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPRPPDPEEPLHLAQSIAAMK
HHHCCCCCHHHHHCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LKYVVITSVDRDDLRDGGAQHFVDCIREVRAHSPQTKIEILVPDFRGRLDIALEKLFACP
HEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PDVMNHNLETVPRLYRQCRPGADYTHSLRLLKEFKARFPGIPTKSGLMLGLGETDEEILD
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHH
VMRDLRKHDVEMLTIGQYLQPSIGHLPVMRYVTPGAFKEFERAAIEMGFSNAACGPMVRS
HHHHHHHCCCHHEEHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
SYHADQQAHEAGIIQR
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TIESRQKGVAKTARNPVKIAPQSTDQLLRKPSWIRVRSSNSQEFYEVKRILREQKLHTV
CCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CEEASCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPRPPDPEEPLHLAQSIAAMK
HHHCCCCCHHHHHCCCCHHEEEHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LKYVVITSVDRDDLRDGGAQHFVDCIREVRAHSPQTKIEILVPDFRGRLDIALEKLFACP
HEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PDVMNHNLETVPRLYRQCRPGADYTHSLRLLKEFKARFPGIPTKSGLMLGLGETDEEILD
HHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHH
VMRDLRKHDVEMLTIGQYLQPSIGHLPVMRYVTPGAFKEFERAAIEMGFSNAACGPMVRS
HHHHHHHCCCHHEEHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHH
SYHADQQAHEAGIIQR
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA