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The map label for this gene is yjfH [C]

Identifier: 78779779

GI number: 78779779

Start: 1309418

End: 1310131

Strand: Reverse

Name: yjfH [C]

Synonym: PMT9312_1395

Alternate gene names: 78779779

Gene position: 1310131-1309418 (Counterclockwise)

Preceding gene: 78779783

Following gene: 78779778

Centisome position: 76.65

GC content: 34.59

Gene sequence:

>714_bases
TTGTTGAAGTCTGGAAAAACTCCCTCCAAAATTTTAGTTACCGAAAAATGGCTAAGAAAGAATGAAAATCTAAGCAAAAA
GCTTCAACCGTCATTAATAAATATTGTCTCAGAGGAAGTCTTAGCATCTGCGATTTCAACGATTAATCCAGATGGGATAG
CAGCTTTGGTAGAGATTTCATCAATACCTAATTATCAATTTAATATTAAAGATGATTTTGTTCTTGTTCTCGAAAGGATT
CAAGATCCTGGGAATATGGGTAGTCTTTTCAGAACTGCTCTAGCTGCTGGCGTTGATGCAATTCTTTTAGCTGGAGGTGC
CCATCCATTAAGCCAAAAGGTATTAAGGGCATCTTCTGGCGCAGTTTTTCATCTGCCATTTTTAAGATTTGATGGAACTG
AAGAAGAAATATTAAATTCTTTATGCAAGTCTTTGTATGAATTATCAAATGTAGGATTTAAGATTTTTACTACTAGTAGC
CATAATAAAAGTTCAAAAAAATCTTCAAAACCCTACTGGGAAATTGATTGGACTAAGTCTACTGCCTTGATTTTGGGTAA
TGAGGGTCAAGGTATTCATAAAAAAATTCAAGAAGCTTTTAATGAAACAATTACAATTCCGCATAGTGAGCTTGTAGAAT
CATTAAATGTGGCTTGCGTTGCAGTTCCATTATTACTAGAACGAAAAAGAGTCGCATACACCTCTAATACGTAG

Upstream 100 bases:

>100_bases
AAACAATAATCTAGTTAAAAGATTTAGGTCCTTTAAAAGAGGGTTCTCTAGAAAAGAACAAGATTTTTTTTGCATAGAGG
GTACTCATCTCATTGAAGAA

Downstream 100 bases:

>100_bases
ATAAAAAGTGACTGATTCAAGTTTTGATTTTGATTTAATCGTAATAGGAGCAGGATATGGAGGTTTTGATGCCGCTAAAC
ATGCTGCTGGTAAGGGACTG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLKSGKTPSKILVTEKWLRKNENLSKKLQPSLINIVSEEVLASAISTINPDGIAALVEISSIPNYQFNIKDDFVLVLERI
QDPGNMGSLFRTALAAGVDAILLAGGAHPLSQKVLRASSGAVFHLPFLRFDGTEEEILNSLCKSLYELSNVGFKIFTTSS
HNKSSKKSSKPYWEIDWTKSTALILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNT

Sequences:

>Translated_237_residues
MLKSGKTPSKILVTEKWLRKNENLSKKLQPSLINIVSEEVLASAISTINPDGIAALVEISSIPNYQFNIKDDFVLVLERI
QDPGNMGSLFRTALAAGVDAILLAGGAHPLSQKVLRASSGAVFHLPFLRFDGTEEEILNSLCKSLYELSNVGFKIFTTSS
HNKSSKKSSKPYWEIDWTKSTALILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNT
>Mature_237_residues
MLKSGKTPSKILVTEKWLRKNENLSKKLQPSLINIVSEEVLASAISTINPDGIAALVEISSIPNYQFNIKDDFVLVLERI
QDPGNMGSLFRTALAAGVDAILLAGGAHPLSQKVLRASSGAVFHLPFLRFDGTEEEILNSLCKSLYELSNVGFKIFTTSS
HNKSSKKSSKPYWEIDWTKSTALILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNT

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=172, Percent_Identity=26.7441860465116, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26022; Mature: 26022

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSGKTPSKILVTEKWLRKNENLSKKLQPSLINIVSEEVLASAISTINPDGIAALVEIS
CCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHH
SIPNYQFNIKDDFVLVLERIQDPGNMGSLFRTALAAGVDAILLAGGAHPLSQKVLRASSG
CCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEECCCCHHHHHHHHHCCC
AVFHLPFLRFDGTEEEILNSLCKSLYELSNVGFKIFTTSSHNKSSKKSSKPYWEIDWTKS
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCC
TALILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNT
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MLKSGKTPSKILVTEKWLRKNENLSKKLQPSLINIVSEEVLASAISTINPDGIAALVEIS
CCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHH
SIPNYQFNIKDDFVLVLERIQDPGNMGSLFRTALAAGVDAILLAGGAHPLSQKVLRASSG
CCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHCHHEEEEECCCCHHHHHHHHHCCC
AVFHLPFLRFDGTEEEILNSLCKSLYELSNVGFKIFTTSSHNKSSKKSSKPYWEIDWTKS
CEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEEEEEECCC
TALILGNEGQGIHKKIQEAFNETITIPHSELVESLNVACVAVPLLLERKRVAYTSNT
EEEEECCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]