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The map label for this gene is lpdA [H]

Identifier: 78779778

GI number: 78779778

Start: 1307971

End: 1309410

Strand: Reverse

Name: lpdA [H]

Synonym: PMT9312_1394

Alternate gene names: 78779778

Gene position: 1309410-1307971 (Counterclockwise)

Preceding gene: 78779779

Following gene: 78779777

Centisome position: 76.61

GC content: 35.69

Gene sequence:

>1440_bases
GTGACTGATTCAAGTTTTGATTTTGATTTAATCGTAATAGGAGCAGGATATGGAGGTTTTGATGCCGCTAAACATGCTGC
TGGTAAGGGACTGAAAGTCGCAATAGTAGAATCTTCTGACATGGGAGGCACTTGTGTTAACAAGGGGTGTGTCCCCTCTA
AAGCTCTTTTGGCTGCAAGTGGAAAAGTCAGAGAAATTGCTGATTATGAACATTTAGCAAAATTTGGTATTCATGCTTCC
CCAGTAAGGTTTGAAAGATCAAAAATTGCAGATCATGCAAATAATTTAGTTTTAAATGTTAGAGAAAATTTAACAAAAAC
TCTCAAAAGGAGTGGAGTTGAAATTATTTTGGGCATTGGCAGAATTGAAGGAAATCAAAAAGTAGGTGTAAGAGATAAAA
ACGGAATTGATAAAATCTTCACATGTAAGAATATTGTTATAGCAACTGGTTCTTCTCCTTTTGTTCCTCGTGGAATAACT
TTGGATAATAGGACTGTATTTACTAGTGATGATGCTGTTAAACTTGAGTGGCTTCCAAGATGGATAGCTATTATTGGAAG
CGGATATATAGGATTGGAATTTGCTGATGTTTATACTGCGCTAGGTTGTGAAGTTACCATGATTGAGGCTTTGGAAAATA
TTATGCCAACATTTGATCCAGACATTACAAAAATTGCTAAGAAGAATCTTATTCAAGCTAGAGATATAGACACAAAATCA
AATGTCTTTGCTACAAAAATAACACCTGGATGTCCTGTGAAAATAGAACTGACAGATGCAAAATCTAAAGAAGTTGTAGA
AACTTTAGAAGTTGATGCCGTATTAGTTGCAACTGGCAGAAGTCCTAATAGTAATAACTTAAATCTTGAGTCGGCTGGGA
TAGAAACAGTAAAAGGTTTCATTCCTATAGATGATCAAATGAGGGTTAAGAATGGTGATGAAATAATACCTAACATTTGG
GCTGTTGGAGATGTAACAGGCAAACTAATGCTTGCCCATACAGCTGCAGCGCAAGGTACTATTGCTGTTGATAATATTTG
CGGTGGTAACGTCGAAATTAACTATAAAAGTATCCCTGCAGCAACCTTTACTCATCCTGAAATAAGTTCAGTTGGTCTCT
CTGAAGCAGAAGCTAAAGAGATATCTGCAAAAGAAAATTTTACTTTGGGAGTTGTCAAAAGTTTCTTTAAGGCTAATTCA
AAAGCATTGGCTGAATTGGAGAGTGATGGATTGCTAAAGTTGATTTTCAATAAAGATAATGGGAAAGTATTAGGTGCTCA
TATTTTTGGGTTACATGCAGCTGATTTAATTCAAGAAATTTCGAACGCTATTTCAAGGAACCAAGATGTAATTGAATTAT
CTACAGAAGTTCATACTCATCCCACTCTTAGTGAAGTGGTTGAGGTCGCATATAAACAAGCGGCTTCTCAAATAAAATAA

Upstream 100 bases:

>100_bases
CATAGTGAGCTTGTAGAATCATTAAATGTGGCTTGCGTTGCAGTTCCATTATTACTAGAACGAAAAAGAGTCGCATACAC
CTCTAATACGTAGATAAAAA

Downstream 100 bases:

>100_bases
TCCCTATAATTAATGGAGATAAGACGCAGGCCACCAAATCCAACAGTAAGGGTAGAAAATTTAGAATATGCCGTGCCTCA
TAGAGAAGCACAAGCAAAAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHAS
PVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGIT
LDNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
NVFATKITPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESAGIETVKGFIPIDDQMRVKNGDEIIPNIW
AVGDVTGKLMLAHTAAAQGTIAVDNICGGNVEINYKSIPAATFTHPEISSVGLSEAEAKEISAKENFTLGVVKSFFKANS
KALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIELSTEVHTHPTLSEVVEVAYKQAASQIK

Sequences:

>Translated_479_residues
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHAS
PVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGIT
LDNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
NVFATKITPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESAGIETVKGFIPIDDQMRVKNGDEIIPNIW
AVGDVTGKLMLAHTAAAQGTIAVDNICGGNVEINYKSIPAATFTHPEISSVGLSEAEAKEISAKENFTLGVVKSFFKANS
KALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIELSTEVHTHPTLSEVVEVAYKQAASQIK
>Mature_478_residues
TDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAASGKVREIADYEHLAKFGIHASP
VRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIGRIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITL
DNRTVFTSDDAVKLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKSN
VFATKITPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESAGIETVKGFIPIDDQMRVKNGDEIIPNIWA
VGDVTGKLMLAHTAAAQGTIAVDNICGGNVEINYKSIPAATFTHPEISSVGLSEAEAKEISAKENFTLGVVKSFFKANSK
ALAELESDGLLKLIFNKDNGKVLGAHIFGLHAADLIQEISNAISRNQDVIELSTEVHTHPTLSEVVEVAYKQAASQIK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=478, Percent_Identity=35.9832635983264, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=30.4904051172708, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI291045266, Length=485, Percent_Identity=29.0721649484536, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=483, Percent_Identity=26.7080745341615, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519428, Length=483, Percent_Identity=26.7080745341615, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI33519426, Length=483, Percent_Identity=26.7080745341615, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI148277065, Length=483, Percent_Identity=26.7080745341615, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI148277071, Length=482, Percent_Identity=26.5560165975104, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=27.5210084033613, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI291045268, Length=483, Percent_Identity=26.7080745341615, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1786307, Length=463, Percent_Identity=33.2613390928726, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=28.6315789473684, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789915, Length=452, Percent_Identity=28.7610619469027, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=28.6324786324786, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=485, Percent_Identity=32.5773195876289, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=30.6209850107066, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=30.6209850107066, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=28.8617886178862, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=28.1059063136456, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=479, Percent_Identity=34.0292275574113, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6325166, Length=486, Percent_Identity=28.1893004115226, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=490, Percent_Identity=27.7551020408163, Blast_Score=150, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21358499, Length=481, Percent_Identity=35.5509355509356, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24640553, Length=497, Percent_Identity=28.9738430583501, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24640549, Length=495, Percent_Identity=29.0909090909091, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=28.952772073922, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737741, Length=502, Percent_Identity=26.0956175298805, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51341; Mature: 51210

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAAS
CCCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCC
GKVREIADYEHLAKFGIHASPVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIG
CCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEEEEEE
RIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITLDNRTVFTSDDAVKLEWLPR
EECCCCEECCCCCCCCCCEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCEEEEHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
HHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
NVFATKITPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESAGIETVKGF
CEEEEECCCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCEECE
IPIDDQMRVKNGDEIIPNIWAVGDVTGKLMLAHTAAAQGTIAVDNICGGNVEINYKSIPA
ECCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEEECCC
ATFTHPEISSVGLSEAEAKEISAKENFTLGVVKSFFKANSKALAELESDGLLKLIFNKDN
EEECCCCCHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GKVLGAHIFGLHAADLIQEISNAISRNQDVIELSTEVHTHPTLSEVVEVAYKQAASQIK
CEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDSSFDFDLIVIGAGYGGFDAAKHAAGKGLKVAIVESSDMGGTCVNKGCVPSKALLAAS
CCCCCCEEEEEEECCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHCCC
GKVREIADYEHLAKFGIHASPVRFERSKIADHANNLVLNVRENLTKTLKRSGVEIILGIG
CCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEEEEEE
RIEGNQKVGVRDKNGIDKIFTCKNIVIATGSSPFVPRGITLDNRTVFTSDDAVKLEWLPR
EECCCCEECCCCCCCCCCEEEECEEEEECCCCCCCCCCEEECCCEEEECCCCEEEEHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALENIMPTFDPDITKIAKKNLIQARDIDTKS
HHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
NVFATKITPGCPVKIELTDAKSKEVVETLEVDAVLVATGRSPNSNNLNLESAGIETVKGF
CEEEEECCCCCCEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCEEECCCCCCEECE
IPIDDQMRVKNGDEIIPNIWAVGDVTGKLMLAHTAAAQGTIAVDNICGGNVEINYKSIPA
ECCCCCEEECCCCCCCCCEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEEECCC
ATFTHPEISSVGLSEAEAKEISAKENFTLGVVKSFFKANSKALAELESDGLLKLIFNKDN
EEECCCCCHHCCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEECCC
GKVLGAHIFGLHAADLIQEISNAISRNQDVIELSTEVHTHPTLSEVVEVAYKQAASQIK
CEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]