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The map label for this gene is pilT [H]

Identifier: 78779571

GI number: 78779571

Start: 1107438

End: 1108514

Strand: Reverse

Name: pilT [H]

Synonym: PMT9312_1188

Alternate gene names: 78779571

Gene position: 1108514-1107438 (Counterclockwise)

Preceding gene: 78779572

Following gene: 78779570

Centisome position: 64.86

GC content: 34.73

Gene sequence:

>1077_bases
ATGAAATTAACACAATTAATGTCTGATTTAGTTAAAAGAAATGGGTCAGATTTACATCTGACTGGCAATTCTGTTCCCTT
TTTTAGGGTGCAAGGTCAAATAGTGCCTGCTGATTCAGAAATTTTCTCTTCTGAAGAATTAAAGGATCAATTAACTAAAA
TTCTCGGAGGTGAGAAAATGGTTAACTTTGAGAAAGAAAAAGAATTGGATTGTAGTTATGGTCTTAAAGGAATTGCGCGA
TTCAGAATAAATATCTTTTTTGATAGGGGTAATATTTCATGTGTGATGAGGGCTTTAAGTACTTCCATTCCAAGTTTTTC
TAAAATTGGATTGCCTGATAGTGTTCAGCAACTATTAAATAGACCAAGAGGCCTAATGCTGGTAACCGGACCCACCGGTT
CTGGTAAGACTACTACATTAGCAAGTTCAATTGATTGGATAAATAGTAATAAAGCCCATCATATTCTTACCATTGAAGAT
CCCATAGAGTTTATTTATGAAAACAAAAATTGTCTTATTAGGCAAAGAGAAGTAGGTGAAGACACATACTCTTTTTCAAA
AGCTTTGAGATCCGCTCTTCGAGAAGATCCGGATATTATTCTCGTTGGCGAGATGAGAGATTTGGAAACAATAAGTCTTG
CTATAACAGCAGCTGAAACTGGACATTTAGTAATGGGAACCCTTCATACTGCCTCAGCATCGCAGACCATTGATAGGATT
ATTGATGTTTTCCCCACCTCTCAACAAACACAAGTTAGAGTTCAACTTTCTTCTTCTTTGATAGGAGTAATTTCTCAAAC
ACTATGTAAAACTATTGACAATAAAAGATCTTTAGCGGCTGAAATTCTTGTGAATAATAATGCAATTGCTAATTTAATCA
GGGAAGCAAAGTCGTCTCAAGTATATTCTCAACTTCAAATTGGAACAAAATTTGGGATGCAAACCTTAGAGCAACATTTA
TCTAACTTAGTAAAGAGGGGAGTAATCACAAATGAAGAGGCAATTTATAAGTGCAATAGACCAACAGTTTTAAAAGGACT
TTTAGAGGAATTAAACCCTACGTCTAAAGAAGAATAA

Upstream 100 bases:

>100_bases
GTTTATTAGATTATGGAATGGAACTTGTAAAAAAAGAATTAACTACTATTGAAGAAGTAGAAAGAGTATGCCTGCTTGAG
GAGCCATTACCTGAGGAATC

Downstream 100 bases:

>100_bases
ATTAGATGAAATACTATTACATAACTGACTTAAAATTAAGAAATGTAATGCTTTTAGCTAATAAAAAGAGGAAATTTTCT
GGAAATTCGATAAAAATATC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKLTQLMSDLVKRNGSDLHLTGNSVPFFRVQGQIVPADSEIFSSEELKDQLTKILGGEKMVNFEKEKELDCSYGLKGIAR
FRINIFFDRGNISCVMRALSTSIPSFSKIGLPDSVQQLLNRPRGLMLVTGPTGSGKTTTLASSIDWINSNKAHHILTIED
PIEFIYENKNCLIRQREVGEDTYSFSKALRSALREDPDIILVGEMRDLETISLAITAAETGHLVMGTLHTASASQTIDRI
IDVFPTSQQTQVRVQLSSSLIGVISQTLCKTIDNKRSLAAEILVNNNAIANLIREAKSSQVYSQLQIGTKFGMQTLEQHL
SNLVKRGVITNEEAIYKCNRPTVLKGLLEELNPTSKEE

Sequences:

>Translated_358_residues
MKLTQLMSDLVKRNGSDLHLTGNSVPFFRVQGQIVPADSEIFSSEELKDQLTKILGGEKMVNFEKEKELDCSYGLKGIAR
FRINIFFDRGNISCVMRALSTSIPSFSKIGLPDSVQQLLNRPRGLMLVTGPTGSGKTTTLASSIDWINSNKAHHILTIED
PIEFIYENKNCLIRQREVGEDTYSFSKALRSALREDPDIILVGEMRDLETISLAITAAETGHLVMGTLHTASASQTIDRI
IDVFPTSQQTQVRVQLSSSLIGVISQTLCKTIDNKRSLAAEILVNNNAIANLIREAKSSQVYSQLQIGTKFGMQTLEQHL
SNLVKRGVITNEEAIYKCNRPTVLKGLLEELNPTSKEE
>Mature_358_residues
MKLTQLMSDLVKRNGSDLHLTGNSVPFFRVQGQIVPADSEIFSSEELKDQLTKILGGEKMVNFEKEKELDCSYGLKGIAR
FRINIFFDRGNISCVMRALSTSIPSFSKIGLPDSVQQLLNRPRGLMLVTGPTGSGKTTTLASSIDWINSNKAHHILTIED
PIEFIYENKNCLIRQREVGEDTYSFSKALRSALREDPDIILVGEMRDLETISLAITAAETGHLVMGTLHTASASQTIDRI
IDVFPTSQQTQVRVQLSSSLIGVISQTLCKTIDNKRSLAAEILVNNNAIANLIREAKSSQVYSQLQIGTKFGMQTLEQHL
SNLVKRGVITNEEAIYKCNRPTVLKGLLEELNPTSKEE

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=40.3669724770642, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI1789723, Length=159, Percent_Identity=44.0251572327044, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=33.6879432624113, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39700; Mature: 39700

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTQLMSDLVKRNGSDLHLTGNSVPFFRVQGQIVPADSEIFSSEELKDQLTKILGGEKM
CCHHHHHHHHHHCCCCEEEEECCCCCEEEEECEEECCCHHHHCHHHHHHHHHHHHCCCCE
VNFEKEKELDCSYGLKGIARFRINIFFDRGNISCVMRALSTSIPSFSKIGLPDSVQQLLN
ECCCHHCCCCHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RPRGLMLVTGPTGSGKTTTLASSIDWINSNKAHHILTIEDPIEFIYENKNCLIRQREVGE
CCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEEEHHCCC
DTYSFSKALRSALREDPDIILVGEMRDLETISLAITAAETGHLVMGTLHTASASQTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPTSQQTQVRVQLSSSLIGVISQTLCKTIDNKRSLAAEILVNNNAIANLIREAKSSQ
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHHHHHHH
VYSQLQIGTKFGMQTLEQHLSNLVKRGVITNEEAIYKCNRPTVLKGLLEELNPTSKEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKLTQLMSDLVKRNGSDLHLTGNSVPFFRVQGQIVPADSEIFSSEELKDQLTKILGGEKM
CCHHHHHHHHHHCCCCEEEEECCCCCEEEEECEEECCCHHHHCHHHHHHHHHHHHCCCCE
VNFEKEKELDCSYGLKGIARFRINIFFDRGNISCVMRALSTSIPSFSKIGLPDSVQQLLN
ECCCHHCCCCHHHCCCEEEEEEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RPRGLMLVTGPTGSGKTTTLASSIDWINSNKAHHILTIEDPIEFIYENKNCLIRQREVGE
CCCCEEEEECCCCCCCCEEEHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEEEHHCCC
DTYSFSKALRSALREDPDIILVGEMRDLETISLAITAAETGHLVMGTLHTASASQTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCHHHHHHHHH
IDVFPTSQQTQVRVQLSSSLIGVISQTLCKTIDNKRSLAAEILVNNNAIANLIREAKSSQ
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHEECCHHHHHHHHHHHHHH
VYSQLQIGTKFGMQTLEQHLSNLVKRGVITNEEAIYKCNRPTVLKGLLEELNPTSKEE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]