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The map label for this gene is pilC [H]

Identifier: 78779570

GI number: 78779570

Start: 1106092

End: 1107432

Strand: Reverse

Name: pilC [H]

Synonym: PMT9312_1187

Alternate gene names: 78779570

Gene position: 1107432-1106092 (Counterclockwise)

Preceding gene: 78779571

Following gene: 78779568

Centisome position: 64.79

GC content: 30.05

Gene sequence:

>1341_bases
ATGAAATACTATTACATAACTGACTTAAAATTAAGAAATGTAATGCTTTTAGCTAATAAAAAGAGGAAATTTTCTGGAAA
TTCGATAAAAATATCTAAAAGCAAAAATAAGATTAAAAAAATTCCACAAATTTCTCCAGAAAAAAGAAAAAAATATCAAA
GTAAAGTTTATGCACTTCAGACATATATGGAGGAAAGCAAGCAAAGATCAAAAATAAGAGCTGAGGGTAAGATGGGTGCA
TTAAGAGGAGATGGCCAAAAAATAAAAAAACCAAAAAAAATGAACAGTAAAAAACTAAAATCCCCTCCAACAAAAGATCT
TGCCGTTACAACTAAACAATTATCATCAATGATCAAGACTGGTTTACCTCTATTAGATTCATTAAATATAATTTCTGAAA
CATCTGAGAATGTAACTATCAAATATGTATTTAAAGAGACATCCTTGGGAATAAGTAAAGGATTATCTATGTCTGAGAAT
TTAGAGAAATATCCAGAGGTTTTTAATGAAATGTATCTTGCTCTTGTATCTGCTGGAGAAACGGCAGGATTATTGCCAGA
TACTCTAGACAGAGAGGCCAAATTGCTTGAGAGTTTAGCAAAAATTAAGGGTCAAATTAAAAGTGCAATGGCCTATCCAA
TCGCTATATTTACATTGACTATTGTTGTTGTTATAGCAATGTTGATTTTTGTTATACCAATATTTGTTGATATCTATGAA
CAATCTAATGCCAAATTGCCGACCATAACTCAATTATTAGTAGATGCATCGAATCAATTAATTAGTTTAGATTTTTATAT
AAAAGCTATTCCAACAGTTTTTATTTTGTTCCAAGCATATAAATATATTTCGAGAAGACCTTCAGTTTTGTGGTGGTTTG
ATAGATCTATGTTATACCTTCCTGTTGTTAAAGATCTTGTAACTAAGTCATGCTTAGCAAATTTTTCAAGAACTCTTTCT
GCATTGAATTCAGCAGGAGTTCCAATTTTAGAAGCTCTTTCTATTTCTAGGAAGACAATATCAAATAGAATTTTTAAAAA
AATCATAGATAACATGTATAAAGATATTCAATCTGGCAATCCAATTTATAAGGTTTTGGAAAAAGAGATGGTAATTCCAG
TAATGTTTACATCAATGTTTCGTATTGGAGAAGAAACTGGGGAATTAAGCCAAATGATCAATAAATTAGCTGATTTTTAC
GAGGAAGAGGTTACGGCAAGTGTTAAGACTTTGACTTCAATTATGGAACCTCTATTAATCGTTTTTGTAGCAGTTATTGT
AGCGGTTATATTAATAGCTATGTATCTACCTATGTTTAGTATGATGGATACAGTTGGCTAA

Upstream 100 bases:

>100_bases
TAATCACAAATGAAGAGGCAATTTATAAGTGCAATAGACCAACAGTTTTAAAAGGACTTTTAGAGGAATTAAACCCTACG
TCTAAAGAAGAATAAATTAG

Downstream 100 bases:

>100_bases
AATCGAAAAAAATAGTATTAAATATTTTTTATTGAAAAATAATTTTAAGAAAAATATCCTCACCAAATAACCAAATTAAA
AATCCACTAATAGATAAAAA

Product: Type II secretory pathway component PulF-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKYYYITDLKLRNVMLLANKKRKFSGNSIKISKSKNKIKKIPQISPEKRKKYQSKVYALQTYMEESKQRSKIRAEGKMGA
LRGDGQKIKKPKKMNSKKLKSPPTKDLAVTTKQLSSMIKTGLPLLDSLNIISETSENVTIKYVFKETSLGISKGLSMSEN
LEKYPEVFNEMYLALVSAGETAGLLPDTLDREAKLLESLAKIKGQIKSAMAYPIAIFTLTIVVVIAMLIFVIPIFVDIYE
QSNAKLPTITQLLVDASNQLISLDFYIKAIPTVFILFQAYKYISRRPSVLWWFDRSMLYLPVVKDLVTKSCLANFSRTLS
ALNSAGVPILEALSISRKTISNRIFKKIIDNMYKDIQSGNPIYKVLEKEMVIPVMFTSMFRIGEETGELSQMINKLADFY
EEEVTASVKTLTSIMEPLLIVFVAVIVAVILIAMYLPMFSMMDTVG

Sequences:

>Translated_446_residues
MKYYYITDLKLRNVMLLANKKRKFSGNSIKISKSKNKIKKIPQISPEKRKKYQSKVYALQTYMEESKQRSKIRAEGKMGA
LRGDGQKIKKPKKMNSKKLKSPPTKDLAVTTKQLSSMIKTGLPLLDSLNIISETSENVTIKYVFKETSLGISKGLSMSEN
LEKYPEVFNEMYLALVSAGETAGLLPDTLDREAKLLESLAKIKGQIKSAMAYPIAIFTLTIVVVIAMLIFVIPIFVDIYE
QSNAKLPTITQLLVDASNQLISLDFYIKAIPTVFILFQAYKYISRRPSVLWWFDRSMLYLPVVKDLVTKSCLANFSRTLS
ALNSAGVPILEALSISRKTISNRIFKKIIDNMYKDIQSGNPIYKVLEKEMVIPVMFTSMFRIGEETGELSQMINKLADFY
EEEVTASVKTLTSIMEPLLIVFVAVIVAVILIAMYLPMFSMMDTVG
>Mature_446_residues
MKYYYITDLKLRNVMLLANKKRKFSGNSIKISKSKNKIKKIPQISPEKRKKYQSKVYALQTYMEESKQRSKIRAEGKMGA
LRGDGQKIKKPKKMNSKKLKSPPTKDLAVTTKQLSSMIKTGLPLLDSLNIISETSENVTIKYVFKETSLGISKGLSMSEN
LEKYPEVFNEMYLALVSAGETAGLLPDTLDREAKLLESLAKIKGQIKSAMAYPIAIFTLTIVVVIAMLIFVIPIFVDIYE
QSNAKLPTITQLLVDASNQLISLDFYIKAIPTVFILFQAYKYISRRPSVLWWFDRSMLYLPVVKDLVTKSCLANFSRTLS
ALNSAGVPILEALSISRKTISNRIFKKIIDNMYKDIQSGNPIYKVLEKEMVIPVMFTSMFRIGEETGELSQMINKLADFY
EEEVTASVKTLTSIMEPLLIVFVAVIVAVILIAMYLPMFSMMDTVG

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=382, Percent_Identity=28.0104712041885, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786295, Length=334, Percent_Identity=27.8443113772455, Blast_Score=144, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 50393; Mature: 50393

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYYITDLKLRNVMLLANKKRKFSGNSIKISKSKNKIKKIPQISPEKRKKYQSKVYALQ
CCEEEEECHHHHHHHEEECCCCCCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHH
TYMEESKQRSKIRAEGKMGALRGDGQKIKKPKKMNSKKLKSPPTKDLAVTTKQLSSMIKT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHCCCHHCCCCCCCHHHHHHHHHHHHHHH
GLPLLDSLNIISETSENVTIKYVFKETSLGISKGLSMSENLEKYPEVFNEMYLALVSAGE
CCCHHHHHHHHHCCCCCEEEEEEEECHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
TAGLLPDTLDREAKLLESLAKIKGQIKSAMAYPIAIFTLTIVVVIAMLIFVIPIFVDIYE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSNAKLPTITQLLVDASNQLISLDFYIKAIPTVFILFQAYKYISRRPSVLWWFDRSMLYL
CCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
PVVKDLVTKSCLANFSRTLSALNSAGVPILEALSISRKTISNRIFKKIIDNMYKDIQSGN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIYKVLEKEMVIPVMFTSMFRIGEETGELSQMINKLADFYEEEVTASVKTLTSIMEPLLI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVAVIVAVILIAMYLPMFSMMDTVG
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYYYITDLKLRNVMLLANKKRKFSGNSIKISKSKNKIKKIPQISPEKRKKYQSKVYALQ
CCEEEEECHHHHHHHEEECCCCCCCCCEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHH
TYMEESKQRSKIRAEGKMGALRGDGQKIKKPKKMNSKKLKSPPTKDLAVTTKQLSSMIKT
HHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHCCCHHCCCCCCCHHHHHHHHHHHHHHH
GLPLLDSLNIISETSENVTIKYVFKETSLGISKGLSMSENLEKYPEVFNEMYLALVSAGE
CCCHHHHHHHHHCCCCCEEEEEEEECHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
TAGLLPDTLDREAKLLESLAKIKGQIKSAMAYPIAIFTLTIVVVIAMLIFVIPIFVDIYE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSNAKLPTITQLLVDASNQLISLDFYIKAIPTVFILFQAYKYISRRPSVLWWFDRSMLYL
CCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
PVVKDLVTKSCLANFSRTLSALNSAGVPILEALSISRKTISNRIFKKIIDNMYKDIQSGN
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PIYKVLEKEMVIPVMFTSMFRIGEETGELSQMINKLADFYEEEVTASVKTLTSIMEPLLI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVAVIVAVILIAMYLPMFSMMDTVG
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]