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The map label for this gene is epsE [H]

Identifier: 78779572

GI number: 78779572

Start: 1108511

End: 1110295

Strand: Reverse

Name: epsE [H]

Synonym: PMT9312_1189

Alternate gene names: 78779572

Gene position: 1110295-1108511 (Counterclockwise)

Preceding gene: 78779578

Following gene: 78779571

Centisome position: 64.96

GC content: 31.88

Gene sequence:

>1785_bases
ATGGTTAATAAAGAAATTATAGTCAGTGATATTCAAAAGAGTTTGAATAATAGAGTTTGTGAAGATGCTGGAATAATTCC
TGTGAGTATTGAGTTTGGGGTAATTGAAATAGGAGCAATGAATCCAGATTTTATTAGAGTTAAAGAAGTAATAACTGATA
TTAAGAGACGTTTTAATGTTGAAGTTGTATTAAAACAAATAACTGCATCTGAATGGGAAGAATGGTTTGAAAATAATTCA
TCAATATCATTGACTGATGAAAATCAATTAAAAAATGGAATAGAAGATACTAGTGATGAAATAAATATCCAATCAAAATC
AACTGAAGATCTTAAGAGTGATAATCTAAATGATGAAATAGATAATTTTGATATTGTAGAAAATGAGGAAAACAATAATT
CAATAGATATTAATTATTCAAATGAAACTAATAACTTTCATTATGAATCCAAAGAAATTAAAGAAAAGCCATTTGAAGAA
GATGTTGAAAACGATAATGCTTTAATTAAACTCGCACAAACTAATTTAGGTGAAGATTTTGAAAATGATGTAGATAGTTT
TTTTGGAGATGAAATTACAAAATCTAAGGATCCAGTGATATCTGGTGTTGCATCATTGTTAAGTAAGTGTTTCTCTCTTA
ATGCTTCAGATGTACACATAGAACCCCTTGAAGACAGATTAAGAATACGCTATAGAATTGATGGAATGCTCGAAGAGGTA
TTCGCTTTCCCAAGATCTCATATCAGTCCAATTGTAAGCAGACTTAAGATAATGAGTAATTTAGACATAGCTGAAAAAAG
AATACCGCAAGATGGAAGAATAAGATGTTTGCTAAGAGGAAGAAAGTCTGATTTTAGAGTTAGCACACTACCAGGTAAAT
GGGGTGAAAAAGTTGTTCTCAGAGTTTTAGAAAGCGATAGTTCAGTACTTAATCTTTCAAAGTTAATAACTGAAAAAAGT
GAATTAGATTTAATAAGAATAATGTCTAAAACTCCCTATGGAATTGTCGTTGTAGTAGGTCCAACTGGAAGTGGAAAATC
CACTACTTTATACTCTATGTTGAGTGAATTAAACTCTCCTGGAGTCAATATTAGTACTGTTGAGGATCCAGTTGAATACA
CTTTGGATGGTATTCATCAAGTGCAAGTAATTAGAGAGAAAGGTCTTGATTTCTCAAGAGCTTTGAGATCGTTGATGAGG
CAAGATCCAGATATAATTTTAGTAGGTGAGACTAGAGATAAAGAAACTGCCCAAGCCGCCATGGAAGCAGCATTAACTGG
ACATATGGTTTTTACTACTCTTCATGCAAATGATACTGCGACCGCAATTACCCGTCTCTCTGAAATGAATATTCCTCCAT
ATTTGATTGGAGCATCAATCATAGGAGTTGTTGCTCAAAGATTAGTTAGAAAAGTTTGTTTGTCATGTAGTTCTGTTAAA
TCTTTAAGTAAAGGGAAAGATGACAGAGCTATAAAATATGGATTAAATAAAGCAAGAATAATAAATGAAACTAGCAATGA
CTCAAAGAGTAGATCATGTCCAGTTTGTGGTGGGAGCGGATATAAAGGTAGAGTTGGGATTTATGAAGTTATGAAAATAA
ATGAAAATTTGCGAGAATTAATTATGAAAGAAAGTACCGCAGATGTAATAAGGTCAAAAGCTTTTTTAGTAGAAGGAAGG
AGTTTATTAGATTATGGAATGGAACTTGTAAAAAAAGAATTAACTACTATTGAAGAAGTAGAAAGAGTATGCCTGCTTGA
GGAGCCATTACCTGAGGAATCATGA

Upstream 100 bases:

>100_bases
ATGATTATTATAGCTGTATAAATTCTATAAGCCAGAAAATATTTTTTTAATATAATTTATAGTATGGCTTGAAAATTTTT
AATGATACTTTTCACTAATT

Downstream 100 bases:

>100_bases
AATTAACACAATTAATGTCTGATTTAGTTAAAAGAAATGGGTCAGATTTACATCTGACTGGCAATTCTGTTCCCTTTTTT
AGGGTGCAAGGTCAAATAGT

Product: Type II secretory pathway ATPase PulE/Tfp pilus assembly pathway ATPase PilB-like

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MVNKEIIVSDIQKSLNNRVCEDAGIIPVSIEFGVIEIGAMNPDFIRVKEVITDIKRRFNVEVVLKQITASEWEEWFENNS
SISLTDENQLKNGIEDTSDEINIQSKSTEDLKSDNLNDEIDNFDIVENEENNNSIDINYSNETNNFHYESKEIKEKPFEE
DVENDNALIKLAQTNLGEDFENDVDSFFGDEITKSKDPVISGVASLLSKCFSLNASDVHIEPLEDRLRIRYRIDGMLEEV
FAFPRSHISPIVSRLKIMSNLDIAEKRIPQDGRIRCLLRGRKSDFRVSTLPGKWGEKVVLRVLESDSSVLNLSKLITEKS
ELDLIRIMSKTPYGIVVVVGPTGSGKSTTLYSMLSELNSPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMR
QDPDIILVGETRDKETAQAAMEAALTGHMVFTTLHANDTATAITRLSEMNIPPYLIGASIIGVVAQRLVRKVCLSCSSVK
SLSKGKDDRAIKYGLNKARIINETSNDSKSRSCPVCGGSGYKGRVGIYEVMKINENLRELIMKESTADVIRSKAFLVEGR
SLLDYGMELVKKELTTIEEVERVCLLEEPLPEES

Sequences:

>Translated_594_residues
MVNKEIIVSDIQKSLNNRVCEDAGIIPVSIEFGVIEIGAMNPDFIRVKEVITDIKRRFNVEVVLKQITASEWEEWFENNS
SISLTDENQLKNGIEDTSDEINIQSKSTEDLKSDNLNDEIDNFDIVENEENNNSIDINYSNETNNFHYESKEIKEKPFEE
DVENDNALIKLAQTNLGEDFENDVDSFFGDEITKSKDPVISGVASLLSKCFSLNASDVHIEPLEDRLRIRYRIDGMLEEV
FAFPRSHISPIVSRLKIMSNLDIAEKRIPQDGRIRCLLRGRKSDFRVSTLPGKWGEKVVLRVLESDSSVLNLSKLITEKS
ELDLIRIMSKTPYGIVVVVGPTGSGKSTTLYSMLSELNSPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMR
QDPDIILVGETRDKETAQAAMEAALTGHMVFTTLHANDTATAITRLSEMNIPPYLIGASIIGVVAQRLVRKVCLSCSSVK
SLSKGKDDRAIKYGLNKARIINETSNDSKSRSCPVCGGSGYKGRVGIYEVMKINENLRELIMKESTADVIRSKAFLVEGR
SLLDYGMELVKKELTTIEEVERVCLLEEPLPEES
>Mature_594_residues
MVNKEIIVSDIQKSLNNRVCEDAGIIPVSIEFGVIEIGAMNPDFIRVKEVITDIKRRFNVEVVLKQITASEWEEWFENNS
SISLTDENQLKNGIEDTSDEINIQSKSTEDLKSDNLNDEIDNFDIVENEENNNSIDINYSNETNNFHYESKEIKEKPFEE
DVENDNALIKLAQTNLGEDFENDVDSFFGDEITKSKDPVISGVASLLSKCFSLNASDVHIEPLEDRLRIRYRIDGMLEEV
FAFPRSHISPIVSRLKIMSNLDIAEKRIPQDGRIRCLLRGRKSDFRVSTLPGKWGEKVVLRVLESDSSVLNLSKLITEKS
ELDLIRIMSKTPYGIVVVVGPTGSGKSTTLYSMLSELNSPGVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMR
QDPDIILVGETRDKETAQAAMEAALTGHMVFTTLHANDTATAITRLSEMNIPPYLIGASIIGVVAQRLVRKVCLSCSSVK
SLSKGKDDRAIKYGLNKARIINETSNDSKSRSCPVCGGSGYKGRVGIYEVMKINENLRELIMKESTADVIRSKAFLVEGR
SLLDYGMELVKKELTTIEEVERVCLLEEPLPEES

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=441, Percent_Identity=40.3628117913832, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=39.0052356020942, Blast_Score=273, Evalue=3e-74,
Organism=Escherichia coli, GI87082188, Length=166, Percent_Identity=40.9638554216867, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 66570; Mature: 66570

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKEIIVSDIQKSLNNRVCEDAGIIPVSIEFGVIEIGAMNPDFIRVKEVITDIKRRFNV
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEECCCCCCHHHHHHHHHHHHHHCCH
EVVLKQITASEWEEWFENNSSISLTDENQLKNGIEDTSDEINIQSKSTEDLKSDNLNDEI
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEEECCCCCHHHHCCCCCCCC
DNFDIVENEENNNSIDINYSNETNNFHYESKEIKEKPFEEDVENDNALIKLAQTNLGEDF
CCCCEEECCCCCCEEEEEECCCCCCCEECCHHHHCCCCHHHCCCCCEEEEEECCCCCCHH
ENDVDSFFGDEITKSKDPVISGVASLLSKCFSLNASDVHIEPLEDRLRIRYRIDGMLEEV
HHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEHHHHHHHHHH
FAFPRSHISPIVSRLKIMSNLDIAEKRIPQDGRIRCLLRGRKSDFRVSTLPGKWGEKVVL
HHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHH
RVLESDSSVLNLSKLITEKSELDLIRIMSKTPYGIVVVVGPTGSGKSTTLYSMLSELNSP
HHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC
GVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAA
CCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MEAALTGHMVFTTLHANDTATAITRLSEMNIPPYLIGASIIGVVAQRLVRKVCLSCSSVK
HHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSKGKDDRAIKYGLNKARIINETSNDSKSRSCPVCGGSGYKGRVGIYEVMKINENLREL
HHHCCCCCCHHHHCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IMKESTADVIRSKAFLVEGRSLLDYGMELVKKELTTIEEVERVCLLEEPLPEES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVNKEIIVSDIQKSLNNRVCEDAGIIPVSIEFGVIEIGAMNPDFIRVKEVITDIKRRFNV
CCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECEEEECCCCCCHHHHHHHHHHHHHHCCH
EVVLKQITASEWEEWFENNSSISLTDENQLKNGIEDTSDEINIQSKSTEDLKSDNLNDEI
HHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCCCCCEEECCCCCHHHHCCCCCCCC
DNFDIVENEENNNSIDINYSNETNNFHYESKEIKEKPFEEDVENDNALIKLAQTNLGEDF
CCCCEEECCCCCCEEEEEECCCCCCCEECCHHHHCCCCHHHCCCCCEEEEEECCCCCCHH
ENDVDSFFGDEITKSKDPVISGVASLLSKCFSLNASDVHIEPLEDRLRIRYRIDGMLEEV
HHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEHHHHHHHHHH
FAFPRSHISPIVSRLKIMSNLDIAEKRIPQDGRIRCLLRGRKSDFRVSTLPGKWGEKVVL
HHCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCHHHHHH
RVLESDSSVLNLSKLITEKSELDLIRIMSKTPYGIVVVVGPTGSGKSTTLYSMLSELNSP
HHHHCCCHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCC
GVNISTVEDPVEYTLDGIHQVQVIREKGLDFSRALRSLMRQDPDIILVGETRDKETAQAA
CCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
MEAALTGHMVFTTLHANDTATAITRLSEMNIPPYLIGASIIGVVAQRLVRKVCLSCSSVK
HHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLSKGKDDRAIKYGLNKARIINETSNDSKSRSCPVCGGSGYKGRVGIYEVMKINENLREL
HHHCCCCCCHHHHCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IMKESTADVIRSKAFLVEGRSLLDYGMELVKKELTTIEEVERVCLLEEPLPEES
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]