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The map label for this gene is trmFO

Identifier: 78779510

GI number: 78779510

Start: 1041471

End: 1042883

Strand: Reverse

Name: trmFO

Synonym: PMT9312_1127

Alternate gene names: 78779510

Gene position: 1042883-1041471 (Counterclockwise)

Preceding gene: 78779511

Following gene: 78779509

Centisome position: 61.02

GC content: 33.69

Gene sequence:

>1413_bases
TTGATAGATAAAGAGGTAATTGTAATAGGAGCAGGTCTTGCAGGTTCTGAGGCTGCTTGGCAGATAGCAAATGCTGGAGT
ACCAGTTAAACTAGTTGAAATGAGGCCTTACAAATCAACTCCAGCTCATCATACTGGTGAATTTGGAGAATTGGTTTGTA
GTAATAGTTTTGGTGCAATAAGTGCGGATAGAGCTGCAGGTTTATTGCAAAAAGAATTAAGAATTTTTAATTCATTAATA
GTTCAAACAGCAGATAAATTTGCGGTTCCAGCTGGAGGTGCCTTGGCTGTAGATAGATCTAAATTTAGTAATGCTTTGAC
TGAAACTTTATCTAATCATCCTTTAATTAATATAAAAAGAATTGAACAACTTGATCTCCCTAGCAAAGAAAATATAACCA
TCCTCGCTACTGGTCCATTAACTTCAGATGATTTGGCATATAAAATTCAAGATTTTACAGGTATTGATGACTGTCATTTT
TTTGATGCGGCTAGTCCAATTATTTATGGAGATTCTATTGATCACGAGATTGTCTTCAAAGCAAGTAGATATGATAAAGG
AGATCCGGCATATTTGAATTGCCCTATGGATAAATATGAGTACACCAATTTCAGAAACCAATTAATTGAAGGAGAGCAAG
CTAATTTAAAAGATTTTGAAAAAGAATCTGCTAATTTCTTTGAAGCTTGTTTGCCAATAGAAGAAATTGCCAGAAGAGGA
ATTGACACCATGAGATATGGACCTTTGAAATCTATTGGGTTGTGGAATCCAAAATGGGGAGATCTATTTGATAGGGATAA
TAGATTAAAAAAAAGACCCCATGCAATTGTTCAATTGAGGAAAGAAGATTTAGAGGGGAAATTACTTAATATGGTAGGCT
TTCAAACAAACCTTAAGTGGTCAGAGCAGAAAAGAATATTTAGGATTATTCCTGGTTTAGAAAAGGCTGAATTTGTTCGT
TTTGGAGTAATGCATAGAAATACCTTTTTAGAGTCTCCAAAATTGCTTTTGCCAACATTACAATTTATGAAAAGGGAAAG
TCTTTTCGCAGCTGGTCAAATAACGGGTACTGAGGGTTATGCAGCAGCAGCGGCAGGAGGACTGTTAGCAGGTATTAATG
CATCTTTAATTGCTAAGGGTGAAATAGCTGTAACTTTTCCTGATGAATCAATGATTGGCTCTCTTATGAACTTTATAAGT
AATAGAAATAAAATAATGTCTAGTCAGAAAAAAAACAAATTTCAACCTATCCCTGCTTCATTTGGCTTGGTTCCAGAATT
AACTAAACGAATAAAAGATAAAAGATCAAGGTATATTGCTTATCAACAGAGATCTGTTGACGTACTAAATATTTTTAAAA
GAAAATTAGATTATATTTTTAATAAAGATCACACACTTGTCAAAATTAATTAA

Upstream 100 bases:

>100_bases
CCCCAATAATTGCTGCAGTTGCATGGGTTGTTTTCAATATTCAAAAACCAGCAAGAGAGCAATGGGATAGACAATTCGGA
GAGAAATAAAAATTAATTAA

Downstream 100 bases:

>100_bases
AATAAGCTACTTGCATAATAGAAAATGAAATCAAATAATAGAAATTTCGATGCAATTATTATCGGCTCAGGAATTGGGGG
GTTAGTAACTGCATCACAAT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MIDKEVIVIGAGLAGSEAAWQIANAGVPVKLVEMRPYKSTPAHHTGEFGELVCSNSFGAISADRAAGLLQKELRIFNSLI
VQTADKFAVPAGGALAVDRSKFSNALTETLSNHPLINIKRIEQLDLPSKENITILATGPLTSDDLAYKIQDFTGIDDCHF
FDAASPIIYGDSIDHEIVFKASRYDKGDPAYLNCPMDKYEYTNFRNQLIEGEQANLKDFEKESANFFEACLPIEEIARRG
IDTMRYGPLKSIGLWNPKWGDLFDRDNRLKKRPHAIVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRIIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRESLFAAGQITGTEGYAAAAAGGLLAGINASLIAKGEIAVTFPDESMIGSLMNFIS
NRNKIMSSQKKNKFQPIPASFGLVPELTKRIKDKRSRYIAYQQRSVDVLNIFKRKLDYIFNKDHTLVKIN

Sequences:

>Translated_470_residues
MIDKEVIVIGAGLAGSEAAWQIANAGVPVKLVEMRPYKSTPAHHTGEFGELVCSNSFGAISADRAAGLLQKELRIFNSLI
VQTADKFAVPAGGALAVDRSKFSNALTETLSNHPLINIKRIEQLDLPSKENITILATGPLTSDDLAYKIQDFTGIDDCHF
FDAASPIIYGDSIDHEIVFKASRYDKGDPAYLNCPMDKYEYTNFRNQLIEGEQANLKDFEKESANFFEACLPIEEIARRG
IDTMRYGPLKSIGLWNPKWGDLFDRDNRLKKRPHAIVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRIIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRESLFAAGQITGTEGYAAAAAGGLLAGINASLIAKGEIAVTFPDESMIGSLMNFIS
NRNKIMSSQKKNKFQPIPASFGLVPELTKRIKDKRSRYIAYQQRSVDVLNIFKRKLDYIFNKDHTLVKIN
>Mature_470_residues
MIDKEVIVIGAGLAGSEAAWQIANAGVPVKLVEMRPYKSTPAHHTGEFGELVCSNSFGAISADRAAGLLQKELRIFNSLI
VQTADKFAVPAGGALAVDRSKFSNALTETLSNHPLINIKRIEQLDLPSKENITILATGPLTSDDLAYKIQDFTGIDDCHF
FDAASPIIYGDSIDHEIVFKASRYDKGDPAYLNCPMDKYEYTNFRNQLIEGEQANLKDFEKESANFFEACLPIEEIARRG
IDTMRYGPLKSIGLWNPKWGDLFDRDNRLKKRPHAIVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRIIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRESLFAAGQITGTEGYAAAAAGGLLAGINASLIAKGEIAVTFPDESMIGSLMNFIS
NRNKIMSSQKKNKFQPIPASFGLVPELTKRIKDKRSRYIAYQQRSVDVLNIFKRKLDYIFNKDHTLVKIN

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=420, Percent_Identity=23.3333333333333, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=433, Percent_Identity=25.8660508083141, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321202, Length=453, Percent_Identity=24.5033112582781, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PROM9 (Q31AA9)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397622.1
- ProteinModelPortal:   Q31AA9
- SMR:   Q31AA9
- STRING:   Q31AA9
- GeneID:   3765931
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1127
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   PMAR74546:PMT9312_1127-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 52630; Mature: 52630

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKEVIVIGAGLAGSEAAWQIANAGVPVKLVEMRPYKSTPAHHTGEFGELVCSNSFGAI
CCCCEEEEEECCCCCCCHHHEECCCCCCEEEEEECCCCCCCCCCCCCHHHEEECCCCCCC
SADRAAGLLQKELRIFNSLIVQTADKFAVPAGGALAVDRSKFSNALTETLSNHPLINIKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCEEEEEH
IEQLDLPSKENITILATGPLTSDDLAYKIQDFTGIDDCHFFDAASPIIYGDSIDHEIVFK
HHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCEECCCCCCEEECCCCCCEEEEE
ASRYDKGDPAYLNCPMDKYEYTNFRNQLIEGEQANLKDFEKESANFFEACLPIEEIARRG
ECCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
IDTMRYGPLKSIGLWNPKWGDLFDRDNRLKKRPHAIVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCH
SEQKRIFRIIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFMKRESLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECEEECCCCC
AAAAAGGLLAGINASLIAKGEIAVTFPDESMIGSLMNFISNRNKIMSSQKKNKFQPIPAS
HHHHHCCHHCCCCCEEEECCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCC
FGLVPELTKRIKDKRSRYIAYQQRSVDVLNIFKRKLDYIFNKDHTLVKIN
CCCHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MIDKEVIVIGAGLAGSEAAWQIANAGVPVKLVEMRPYKSTPAHHTGEFGELVCSNSFGAI
CCCCEEEEEECCCCCCCHHHEECCCCCCEEEEEECCCCCCCCCCCCCHHHEEECCCCCCC
SADRAAGLLQKELRIFNSLIVQTADKFAVPAGGALAVDRSKFSNALTETLSNHPLINIKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCCCEEEEEH
IEQLDLPSKENITILATGPLTSDDLAYKIQDFTGIDDCHFFDAASPIIYGDSIDHEIVFK
HHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCEECCCCCCEEECCCCCCEEEEE
ASRYDKGDPAYLNCPMDKYEYTNFRNQLIEGEQANLKDFEKESANFFEACLPIEEIARRG
ECCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
IDTMRYGPLKSIGLWNPKWGDLFDRDNRLKKRPHAIVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHCCCCCCCH
SEQKRIFRIIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFMKRESLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECEEECCCCC
AAAAAGGLLAGINASLIAKGEIAVTFPDESMIGSLMNFISNRNKIMSSQKKNKFQPIPAS
HHHHHCCHHCCCCCEEEECCEEEEECCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCC
FGLVPELTKRIKDKRSRYIAYQQRSVDVLNIFKRKLDYIFNKDHTLVKIN
CCCHHHHHHHHHHHHHHEEEHHHCCHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA