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The map label for this gene is rsmE [H]

Identifier: 78779053

GI number: 78779053

Start: 630030

End: 630803

Strand: Direct

Name: rsmE [H]

Synonym: PMT9312_0668

Alternate gene names: 78779053

Gene position: 630030-630803 (Clockwise)

Preceding gene: 78779052

Following gene: 78779054

Centisome position: 36.86

GC content: 26.49

Gene sequence:

>774_bases
ATGGAAGATTTAACAAGATTAATTATTTCCCATGAAAGAATTGAAAATATTAAGAACAATAATTTAGAACTTTCTAAAGA
AGAGGCTCATTACATAAATAAAGTAATGAGGATAAAAAATGGTAAAGAAATATTTATATCTAACGGAAAGGGTTCATTAT
GGAAAGCTATAAAAGTTAAAAATGATTGTTTAGAAATAATTCAATTTAAGAAACCTTATTTATTTCAAGAACAAGAAATT
TACTTATTAGGCATAGCTGTTGTTATACCAAAAAGTGGTTTTGAGGATATTTTAAAAATGTGTACTGAAATAGGAATTGA
TTTTATACAGCCATTATTTTCAGAAAGACAGGTAAATAAAAATTTAAATTTTTCTAGAAAACTTTTAAGATGGAATTCAA
TTATCAATGAAGCAGTTGAGCAAAGTGAGAGATTATGGAAACCATCTATTTTAAATGGTATGGATATTATTGAATGGCTA
AAAAGTAGAGATAATCAAGAAAGAGTTTCAATTTCTATAACTAGAGAAGATACTACTTATGACTTAAATCAATGGATAAG
AAAACAACAAGAAGCTGTAAATAAAAAAGGGGGTATTTTTTGGAATGTAATTGGCCCTGAAGGAGGTTGGTCCTCTAAAG
AAATTGATTTTTTTAATAAAAATAATATTACCTTTGTTAAACTTTCCGACAATATCTTAAGAACTTCAACGGCTAGTATT
AACGCATCATCAATTCTCAATCAGTGGAGAATTGATTTGAAATTAAGGAATTAG

Upstream 100 bases:

>100_bases
TAATTATGTAAAAATTGAAGAGTTAATTATTGGCGGTGAAAATAATGATTGGTCAATTGAAGAACATGAAGATTTAGTTT
TCAAAGATTAAGTGTTTTAG

Downstream 100 bases:

>100_bases
ATAAATATGGATTTAATTAGAAATCAATTTTTTATAGGTTTTTGCATTAATTTTATTTTGATTTATATATTTTGCAAGAT
TCCTTTGATGACAAAAAGTG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MEDLTRLIISHERIENIKNNNLELSKEEAHYINKVMRIKNGKEIFISNGKGSLWKAIKVKNDCLEIIQFKKPYLFQEQEI
YLLGIAVVIPKSGFEDILKMCTEIGIDFIQPLFSERQVNKNLNFSRKLLRWNSIINEAVEQSERLWKPSILNGMDIIEWL
KSRDNQERVSISITREDTTYDLNQWIRKQQEAVNKKGGIFWNVIGPEGGWSSKEIDFFNKNNITFVKLSDNILRTSTASI
NASSILNQWRIDLKLRN

Sequences:

>Translated_257_residues
MEDLTRLIISHERIENIKNNNLELSKEEAHYINKVMRIKNGKEIFISNGKGSLWKAIKVKNDCLEIIQFKKPYLFQEQEI
YLLGIAVVIPKSGFEDILKMCTEIGIDFIQPLFSERQVNKNLNFSRKLLRWNSIINEAVEQSERLWKPSILNGMDIIEWL
KSRDNQERVSISITREDTTYDLNQWIRKQQEAVNKKGGIFWNVIGPEGGWSSKEIDFFNKNNITFVKLSDNILRTSTASI
NASSILNQWRIDLKLRN
>Mature_257_residues
MEDLTRLIISHERIENIKNNNLELSKEEAHYINKVMRIKNGKEIFISNGKGSLWKAIKVKNDCLEIIQFKKPYLFQEQEI
YLLGIAVVIPKSGFEDILKMCTEIGIDFIQPLFSERQVNKNLNFSRKLLRWNSIINEAVEQSERLWKPSILNGMDIIEWL
KSRDNQERVSISITREDTTYDLNQWIRKQQEAVNKKGGIFWNVIGPEGGWSSKEIDFFNKNNITFVKLSDNILRTSTASI
NASSILNQWRIDLKLRN

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=233, Percent_Identity=23.175965665236, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 30076; Mature: 30076

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLTRLIISHERIENIKNNNLELSKEEAHYINKVMRIKNGKEIFISNGKGSLWKAIKVK
CCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHH
NDCLEIIQFKKPYLFQEQEIYLLGIAVVIPKSGFEDILKMCTEIGIDFIQPLFSERQVNK
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHCC
NLNFSRKLLRWNSIINEAVEQSERLWKPSILNGMDIIEWLKSRDNQERVSISITREDTTY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCC
DLNQWIRKQQEAVNKKGGIFWNVIGPEGGWSSKEIDFFNKNNITFVKLSDNILRTSTASI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCEEECCCCCC
NASSILNQWRIDLKLRN
CHHHHHHHEEEEEEECC
>Mature Secondary Structure
MEDLTRLIISHERIENIKNNNLELSKEEAHYINKVMRIKNGKEIFISNGKGSLWKAIKVK
CCHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHH
NDCLEIIQFKKPYLFQEQEIYLLGIAVVIPKSGFEDILKMCTEIGIDFIQPLFSERQVNK
HHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHCC
NLNFSRKLLRWNSIINEAVEQSERLWKPSILNGMDIIEWLKSRDNQERVSISITREDTTY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCC
DLNQWIRKQQEAVNKKGGIFWNVIGPEGGWSSKEIDFFNKNNITFVKLSDNILRTSTASI
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCCCEEEEEECCCEEECCCCCC
NASSILNQWRIDLKLRN
CHHHHHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]