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The map label for this gene is thiO/thiG [H]

Identifier: 78778433

GI number: 78778433

Start: 51234

End: 52343

Strand: Reverse

Name: thiO/thiG [H]

Synonym: PMT9312_0048

Alternate gene names: 78778433

Gene position: 52343-51234 (Counterclockwise)

Preceding gene: 78778435

Following gene: 78778431

Centisome position: 3.06

GC content: 32.25

Gene sequence:

>1110_bases
ATGGCACAAGAAACCAAAAATTCAATATTAATAATTGGCGGAGGACTTTTAGGTTTATCTATTGCTTATGAATTTTCTAG
AAATAACTTCAAAGTTTTAGTTTTAAGCAAAAACAGAAATGAATCAGCTGGATTTGTTGCTGCAGGAATGTTAGCTACTC
ATGCCGAAGGGCTCGAAGATGAATTACTAAAATTTGGCCAAGAAAGTCAAAATCTAATTCCAAAGTGGATAAAAAGTATT
GAACAAGATAGTAATATTAAATGCGGTTTAAAAAAATGTGGCATAGTAGTTCCTTTTAAAAACAAAGAAGATCTTGAAGA
GTTTCCAACTTATAAATATGGAAAATATTTAAATCAAAAAGATCTTCAAACAGAAATTAATGGAATGAATTCTCTTTGGA
AACATGGTTTACTTTTTGAACAAGATGGTCAAATAGACAACCGAAGAAGACTGATGCGTGCTCTTGAGAGAGCATGCTCC
TTGCATGGAGTCGAATTTCAAGAGGGATCAGAAGTAGAGGGTTTGACATTCGAAAAAAACAAAATTACAGGTGCAACAGT
TGTATGTGCCACTGGGGAAATAAAAAAAATTAACTGCGAAAAAGCAATTATTTGCAGCGGTGCTTGGAGTAAAAAAATTT
TTAATAAGATTCCAGTCTTTCCTGTAAAGGGACAAATGCTATCAATACAAGGTCCCACAAATTTTTTGAAAAGAGTTATT
TTTGGTCCAAAAACTTATCTAGTTCCCCGTGATGATGGACTTATTATAGTTGGAGCGACAGTTGAAAAAGATTCAAAATT
TAATCAGGGTAATACTCCTAATGGAATAAAACAACTGCAAGAAGGCATTCGCTCCTTATTGCCAGAAGCTATTAATTGGC
CACAAATGGAACATTGGTGGGGATTTAGACCCTGCACACCAGATCTAAAACCAATAATTGGAAAATCAAAAATTGAAAAT
CTTTTTATAGCTACAGGACATTACAGAAATGGAGTTTTATTTTCTGCAATAACAAGTGATCTTCTTTTGAAAATAGTTCA
AAATAAAAATCTCAAAGAAATAGAAAAAAGCTTTTTAGAAAAATTTAGTTTAGATAGATTTGCGATTTAA

Upstream 100 bases:

>100_bases
CCAAGATTCCAAATTGTTCATTATCAAAAGTCTCTTTATTAATATTATTGGATATAAAGGAAAAGAGGACCAAAACACAA
ATAAAAATATTAATTTTTAA

Downstream 100 bases:

>100_bases
ATTTTAATTTTCAGATCGCCATTTCGAATCAGAAGTGTACCACTCACATAATTCCTCTTCGTTAAACCATAGATCAATTT
CAAATTTTGCTGTATCTTCT

Product: putative thiamine biosynthesis oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MAQETKNSILIIGGGLLGLSIAYEFSRNNFKVLVLSKNRNESAGFVAAGMLATHAEGLEDELLKFGQESQNLIPKWIKSI
EQDSNIKCGLKKCGIVVPFKNKEDLEEFPTYKYGKYLNQKDLQTEINGMNSLWKHGLLFEQDGQIDNRRRLMRALERACS
LHGVEFQEGSEVEGLTFEKNKITGATVVCATGEIKKINCEKAIICSGAWSKKIFNKIPVFPVKGQMLSIQGPTNFLKRVI
FGPKTYLVPRDDGLIIVGATVEKDSKFNQGNTPNGIKQLQEGIRSLLPEAINWPQMEHWWGFRPCTPDLKPIIGKSKIEN
LFIATGHYRNGVLFSAITSDLLLKIVQNKNLKEIEKSFLEKFSLDRFAI

Sequences:

>Translated_369_residues
MAQETKNSILIIGGGLLGLSIAYEFSRNNFKVLVLSKNRNESAGFVAAGMLATHAEGLEDELLKFGQESQNLIPKWIKSI
EQDSNIKCGLKKCGIVVPFKNKEDLEEFPTYKYGKYLNQKDLQTEINGMNSLWKHGLLFEQDGQIDNRRRLMRALERACS
LHGVEFQEGSEVEGLTFEKNKITGATVVCATGEIKKINCEKAIICSGAWSKKIFNKIPVFPVKGQMLSIQGPTNFLKRVI
FGPKTYLVPRDDGLIIVGATVEKDSKFNQGNTPNGIKQLQEGIRSLLPEAINWPQMEHWWGFRPCTPDLKPIIGKSKIEN
LFIATGHYRNGVLFSAITSDLLLKIVQNKNLKEIEKSFLEKFSLDRFAI
>Mature_368_residues
AQETKNSILIIGGGLLGLSIAYEFSRNNFKVLVLSKNRNESAGFVAAGMLATHAEGLEDELLKFGQESQNLIPKWIKSIE
QDSNIKCGLKKCGIVVPFKNKEDLEEFPTYKYGKYLNQKDLQTEINGMNSLWKHGLLFEQDGQIDNRRRLMRALERACSL
HGVEFQEGSEVEGLTFEKNKITGATVVCATGEIKKINCEKAIICSGAWSKKIFNKIPVFPVKGQMLSIQGPTNFLKRVIF
GPKTYLVPRDDGLIIVGATVEKDSKFNQGNTPNGIKQLQEGIRSLLPEAINWPQMEHWWGFRPCTPDLKPIIGKSKIENL
FIATGHYRNGVLFSAITSDLLLKIVQNKNLKEIEKSFLEKFSLDRFAI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=202, Percent_Identity=26.7326732673267, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 41470; Mature: 41338

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQETKNSILIIGGGLLGLSIAYEFSRNNFKVLVLSKNRNESAGFVAAGMLATHAEGLED
CCCCCCCCEEEEECCCEEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHH
ELLKFGQESQNLIPKWIKSIEQDSNIKCGLKKCGIVVPFKNKEDLEEFPTYKYGKYLNQK
HHHHHCCCCCHHHHHHHHHHCCCCCCEECHHHCCEEEECCCHHHHHHCCCCHHHHHCCHH
DLQTEINGMNSLWKHGLLFEQDGQIDNRRRLMRALERACSLHGVEFQEGSEVEGLTFEKN
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
KITGATVVCATGEIKKINCEKAIICSGAWSKKIFNKIPVFPVKGQMLSIQGPTNFLKRVI
CCCCEEEEEECCCEEEECCCEEEEECCCHHHHHHHHCCCEEECCCEEEECCHHHHHHHHH
FGPKTYLVPRDDGLIIVGATVEKDSKFNQGNTPNGIKQLQEGIRSLLPEAINWPQMEHWW
CCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCC
GFRPCTPDLKPIIGKSKIENLFIATGHYRNGVLFSAITSDLLLKIVQNKNLKEIEKSFLE
CCCCCCCCHHHHCCHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KFSLDRFAI
HHCCHHHCC
>Mature Secondary Structure 
AQETKNSILIIGGGLLGLSIAYEFSRNNFKVLVLSKNRNESAGFVAAGMLATHAEGLED
CCCCCCCEEEEECCCEEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCHHH
ELLKFGQESQNLIPKWIKSIEQDSNIKCGLKKCGIVVPFKNKEDLEEFPTYKYGKYLNQK
HHHHHCCCCCHHHHHHHHHHCCCCCCEECHHHCCEEEECCCHHHHHHCCCCHHHHHCCHH
DLQTEINGMNSLWKHGLLFEQDGQIDNRRRLMRALERACSLHGVEFQEGSEVEGLTFEKN
HHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECC
KITGATVVCATGEIKKINCEKAIICSGAWSKKIFNKIPVFPVKGQMLSIQGPTNFLKRVI
CCCCEEEEEECCCEEEECCCEEEEECCCHHHHHHHHCCCEEECCCEEEECCHHHHHHHHH
FGPKTYLVPRDDGLIIVGATVEKDSKFNQGNTPNGIKQLQEGIRSLLPEAINWPQMEHWW
CCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCC
GFRPCTPDLKPIIGKSKIENLFIATGHYRNGVLFSAITSDLLLKIVQNKNLKEIEKSFLE
CCCCCCCCHHHHCCHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KFSLDRFAI
HHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]