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The map label for this gene is trpA

Identifier: 78358517

GI number: 78358517

Start: 3438923

End: 3439735

Strand: Reverse

Name: trpA

Synonym: Dde_3478

Alternate gene names: 78358517

Gene position: 3439735-3438923 (Counterclockwise)

Preceding gene: 78358518

Following gene: 78358515

Centisome position: 92.21

GC content: 62.85

Gene sequence:

>813_bases
ATGCATACAGAACATATCACAAGCCCCGCAGACAGGCTGACCATGCGTATCCGCGAGGCCAACCGTCAGGGCCGCAAGGC
TCTTATCCCGTTTCTTCCGGCAGGGTTTCCCGATTCCGGGGATTTCTGGCGGCAGCTGGACGAGCTGGACGCGGGCGGTG
CCGATGTACTTGAAATAGGCATTCCTTTTTCCGACCCGTGCGCCGACGGACCGGTGGTGGAGCAGGCTTCGCTGGAAAGT
CTGGCCCGCGGAACCACCCTGCGCGGCGTGCTTGAAGAGCTTGAACGGCGCAAGGGACGCTATCGTGCAGGTATTGTGCT
GATGGGGTACCTTAATCCGTTTCTGCAGTACGGGCTGGATGCGCTGGCGCGCGATGCCGCCCGTGCCGGGGTTGAAGGGT
TCATCATTCCCGACCTGCCGCTAGAAGAGGATGCCCTGTGCCGCGGGGCGCTTGCGGCTGAGGGGCTGGCGCTGATTCCG
CTGGTGGCATCCAATACCGATGAAGACCGTATGCGTGCCTATGCCGGAGTGGCGCAGGGCTATGTCTATGTGGTTTCGGT
TATGGGTACCACCGGTGCCCGTACCGACCTGCCTGCGGGGGTTACCGCCACGCTGGAACGCGCCCGCCGGTGCTTTGACC
TGCCGCTGGCGCTGGGGTTCGGCGTCCGTGAACCGGAACAGCTGGCAGCCTGCGGCGACAGACTGGATGCTGTGATTTTC
GGCAGTGCGCTTATCAGCCACATCCGCACACAGGGCAGTGCTGCTTCATTTATGGAACGCTGGCGCCGGCCTGTGGCAGA
ACTGGTGCTCTGA

Upstream 100 bases:

>100_bases
ACGGGATTCCCCGCGGGGCCGATGTGGTCGTCAACCTGTCCGGTCGTGGCGACAAGGACATGGGCATCATCAACAACCTG
CTGTAAAAGGGGAACACACC

Downstream 100 bases:

>100_bases
CGGCGGGCGCGGGGCTGTCAACGGCTTCTGCGCAAAAGAAACAGGGTTTTTTTGACAAAAAAAGTAAAAAGCAGTAAACG
CGAGTTGTCAGTCGCCCTTT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHTEHITSPADRLTMRIREANRQGRKALIPFLPAGFPDSGDFWRQLDELDAGGADVLEIGIPFSDPCADGPVVEQASLES
LARGTTLRGVLEELERRKGRYRAGIVLMGYLNPFLQYGLDALARDAARAGVEGFIIPDLPLEEDALCRGALAAEGLALIP
LVASNTDEDRMRAYAGVAQGYVYVVSVMGTTGARTDLPAGVTATLERARRCFDLPLALGFGVREPEQLAACGDRLDAVIF
GSALISHIRTQGSAASFMERWRRPVAELVL

Sequences:

>Translated_270_residues
MHTEHITSPADRLTMRIREANRQGRKALIPFLPAGFPDSGDFWRQLDELDAGGADVLEIGIPFSDPCADGPVVEQASLES
LARGTTLRGVLEELERRKGRYRAGIVLMGYLNPFLQYGLDALARDAARAGVEGFIIPDLPLEEDALCRGALAAEGLALIP
LVASNTDEDRMRAYAGVAQGYVYVVSVMGTTGARTDLPAGVTATLERARRCFDLPLALGFGVREPEQLAACGDRLDAVIF
GSALISHIRTQGSAASFMERWRRPVAELVL
>Mature_270_residues
MHTEHITSPADRLTMRIREANRQGRKALIPFLPAGFPDSGDFWRQLDELDAGGADVLEIGIPFSDPCADGPVVEQASLES
LARGTTLRGVLEELERRKGRYRAGIVLMGYLNPFLQYGLDALARDAARAGVEGFIIPDLPLEEDALCRGALAAEGLALIP
LVASNTDEDRMRAYAGVAQGYVYVVSVMGTTGARTDLPAGVTATLERARRCFDLPLALGFGVREPEQLAACGDRLDAVIF
GSALISHIRTQGSAASFMERWRRPVAELVL

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=203, Percent_Identity=33.0049261083744, Blast_Score=110, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=39.3305439330544, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29195; Mature: 29195

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTEHITSPADRLTMRIREANRQGRKALIPFLPAGFPDSGDFWRQLDELDAGGADVLEIG
CCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEC
IPFSDPCADGPVVEQASLESLARGTTLRGVLEELERRKGRYRAGIVLMGYLNPFLQYGLD
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCEEHHHHHHHHHHHHHH
ALARDAARAGVEGFIIPDLPLEEDALCRGALAAEGLALIPLVASNTDEDRMRAYAGVAQG
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
YVYVVSVMGTTGARTDLPAGVTATLERARRCFDLPLALGFGVREPEQLAACGDRLDAVIF
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHH
GSALISHIRTQGSAASFMERWRRPVAELVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTEHITSPADRLTMRIREANRQGRKALIPFLPAGFPDSGDFWRQLDELDAGGADVLEIG
CCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEC
IPFSDPCADGPVVEQASLESLARGTTLRGVLEELERRKGRYRAGIVLMGYLNPFLQYGLD
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCEEHHHHHHHHHHHHHH
ALARDAARAGVEGFIIPDLPLEEDALCRGALAAEGLALIPLVASNTDEDRMRAYAGVAQG
HHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
YVYVVSVMGTTGARTDLPAGVTATLERARRCFDLPLALGFGVREPEQLAACGDRLDAVIF
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHH
GSALISHIRTQGSAASFMERWRRPVAELVL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA