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The map label for this gene is ligT [C]

Identifier: 78224713

GI number: 78224713

Start: 3963535

End: 3964089

Strand: Direct

Name: ligT [C]

Synonym: Gmet_3530

Alternate gene names: 78224713

Gene position: 3963535-3964089 (Clockwise)

Preceding gene: 78224712

Following gene: 78224719

Centisome position: 99.15

GC content: 66.67

Gene sequence:

>555_bases
ATGCGCCTGTTCGTGGCAATCGATCTTCCCGACGAGGTCAAACGGTCAGTGGCTGATATCTGCCGGGGAGTCCCCGGAGT
GCGGTGGCTTCCACCGGACCAACTCCATCTGACCCTCCGGTTCATCGGCGAGGAGGACGATGCCGTCGCTGCGGCGATCC
GTCGCGGCCTGGCAGAGATCACCTCTCCCCCCTTCCCCCTTTCCCTCCAGGGGGTGGGCTGCTTCCCTTCCCCCCGGCGT
CCGCGGGTTCTCTGGGTGGGGCTCAGCGGCGGAGCCCCCCTCCAGCAGCTCCAGCAGAAGGTTGAGGCGGCGATGGTAGC
AGCCGGCATCCTTGCCGAAGAGCGCCCCTTCTCACCCCACATCACCCTGGCCCGCCTGAGGGAACACCGGGAGGGTGACG
TCGCCCCCTTCCTGGCGCGGAATGCCTCCTTCCACTGCGAGCCGTTCACGGTCGACGCATTCCACCTCTATTCGAGCATC
CTTGCCGCAAAGGGGGCCATCCACCGGCACGAGGCGAGCTATCCTCTTGCAGGGCGAATGGAGACGGCTGGCTGA

Upstream 100 bases:

>100_bases
GGCACGGTGATTTCGGGTTCTGCGGGGCGCTTCAGGGCAAGAACATCCGGAGCCGACCAGCGCATCGCCACCCGCTGACG
GGTTGGAAAGGAGGCGCGTC

Downstream 100 bases:

>100_bases
CGTTCGGGAGGGGCGAAACAGGGTTAAAGCACGGCCGCCATCCTGAGGGGATGGCGGCCGTGGTCATTGGAGGGATCAGC
TAAAGAGGTCCATGACCTTG

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRLFVAIDLPDEVKRSVADICRGVPGVRWLPPDQLHLTLRFIGEEDDAVAAAIRRGLAEITSPPFPLSLQGVGCFPSPRR
PRVLWVGLSGGAPLQQLQQKVEAAMVAAGILAEERPFSPHITLARLREHREGDVAPFLARNASFHCEPFTVDAFHLYSSI
LAAKGAIHRHEASYPLAGRMETAG

Sequences:

>Translated_184_residues
MRLFVAIDLPDEVKRSVADICRGVPGVRWLPPDQLHLTLRFIGEEDDAVAAAIRRGLAEITSPPFPLSLQGVGCFPSPRR
PRVLWVGLSGGAPLQQLQQKVEAAMVAAGILAEERPFSPHITLARLREHREGDVAPFLARNASFHCEPFTVDAFHLYSSI
LAAKGAIHRHEASYPLAGRMETAG
>Mature_184_residues
MRLFVAIDLPDEVKRSVADICRGVPGVRWLPPDQLHLTLRFIGEEDDAVAAAIRRGLAEITSPPFPLSLQGVGCFPSPRR
PRVLWVGLSGGAPLQQLQQKVEAAMVAAGILAEERPFSPHITLARLREHREGDVAPFLARNASFHCEPFTVDAFHLYSSI
LAAKGAIHRHEASYPLAGRMETAG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=165, Percent_Identity=32.7272727272727, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 20102; Mature: 20102

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFVAIDLPDEVKRSVADICRGVPGVRWLPPDQLHLTLRFIGEEDDAVAAAIRRGLAEI
CEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
TSPPFPLSLQGVGCFPSPRRPRVLWVGLSGGAPLQQLQQKVEAAMVAAGILAEERPFSPH
CCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ITLARLREHREGDVAPFLARNASFHCEPFTVDAFHLYSSILAAKGAIHRHEASYPLAGRM
CHHHHHHHCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC
ETAG
CCCC
>Mature Secondary Structure
MRLFVAIDLPDEVKRSVADICRGVPGVRWLPPDQLHLTLRFIGEEDDAVAAAIRRGLAEI
CEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHH
TSPPFPLSLQGVGCFPSPRRPRVLWVGLSGGAPLQQLQQKVEAAMVAAGILAEERPFSPH
CCCCCCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
ITLARLREHREGDVAPFLARNASFHCEPFTVDAFHLYSSILAAKGAIHRHEASYPLAGRM
CHHHHHHHCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCHHHHCCCCCCCCCCC
ETAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]